Accès ouvert
2026
article
OpenAlex
Ahmed Mahas, Raphaël Ferreira, Lisa Maria Riedmayr, George M. Church
Abstract Programmable technologies that sense specific nucleic acid signatures in living cells and trigger cellular functions hold significant potential for biotechnology and medicine. Here, we developed SONAR ( S ensing O f N ucleic acids using A SOs and R everse-transcriptases), a …
us, dk
(code pays fourni par la source)
Accès ouvert
2026
preprint
OpenAlex
Ahmed Mahas, Ramiro M. Perrotta, Roman Teo Oliynyk, Raphaël Ferreira et autres
Abstract Programmable intracellular production of short functional RNAs underlies diverse applications ranging from gene regulation to genome engineering, but is often constrained by cloning workflows, RNA synthesis, or viral-vector delivery. Here, we introduce a single-stranded DNA (ssDNA)-driven intracellular transcription strategy in which …
us, dk
(code pays fourni par la source)
Accès ouvert
2025
article
OpenAlex
Ramiro M. Perrotta, Svenja Vinke, Raphaël Ferreira, Michaël Moret et autres
Base editors enable precise genome modification but are constrained by bystander edits that limit their applicability. Existing strategies to enhance precision often compromise efficiency and remain highly sequence dependent. Here we present a parallel engineering approach that optimizes both guide RNAs and …
us, dk
(code pays fourni par la source)
Accès ouvert
2025
article
OpenAlex
Roman Teo Oliynyk, Ahmed Mahas, Emil Karpinski
DNA plasmids are widely used for delivering proteins and RNA in genome editing. However, their bacterial components can lead to inactivation, cell toxicity, and reduced efficiency compared to minicircle DNA (mcDNA), which lacks such bacterial sequences. Existing commercial kits that recombine plasmids …
us, nz
(code pays fourni par la source)
Accès ouvert
2024
preprint
OpenAlex
Roman Teo Oliynyk, Ahmed Mahas, Emil Karpinski
Abstract DNA plasmids are widely used for delivering proteins and RNA in genome editing. However, their bacterial components can lead to inactivation, cell toxicity, and reduced efficiency compared to minicircle DNA (mcDNA), which lacks such bacterial sequences. Existing commercial kits that recombine …
us
(code pays fourni par la source)
Accès ouvert
2024
preprint
OpenAlex
Ramiro M. Perrotta, Svenja Vinke, Raphaël Ferreira, Michaël Moret et autres
Abstract As we enter the era of CRISPR medicines, base editors (BEs) emerged as one of the most promising tools to treat genetic associated diseases. However, unintended bystander editing beyond the target nucleotide poses a challenge to their translation into effective therapies. …
us
(code pays fourni par la source)
Accès ouvert
2024
article
OpenAlex
Rashid Aman, Muntjeeb M. Syed, Ahmed A. Saleh, Firdaws Melliti et autres
Gene-editing technologies have revolutionized biotechnology, but current gene editors suffer from several limitations. Here, we harnessed the power of gamma-modified peptide nucleic acids (γPNAs) to facilitate targeted, specific DNA invasion and used T7 endonuclease I (T7EI) to recognize and cleave the γPNA-invaded …
sa, us
(code pays fourni par la source)
Accès ouvert
2023
article
OpenAlex
Tin Maršić, Qiaochu Wang, Rashid Aman, Ahmed Mahas et autres
Programmable site-specific nucleases promise to unlock myriad applications in basic biology research, biotechnology and gene therapy. Gene-editing systems have revolutionized our ability to engineer genomes across diverse eukaryotic species. However, key challenges, including delivery, specificity and targeting organellar genomes, pose barriers to …
sa
(code pays fourni par la source)
Accès ouvert
2022
article
OpenAlex
Ahmed Ghouneimy, Ahmed Mahas, Tin Maršić, Rashid Aman et autres
The COVID-19 pandemic has challenged the conventional diagnostic field and revealed the need for decentralized Point of Care (POC) solutions. Although nucleic acid testing is considered to be the most sensitive and specific disease detection method, conventional testing platforms are expensive, confined …
sa
(code pays fourni par la source)
Accès ouvert
2022
article
OpenAlex
Ahmed Mahas, Tin Maršić, Mauricio Lopez-Portillo Masson, Qiaochu Wang et autres
Type VI CRISPR-Cas systems have been repurposed for various applications such as gene knockdown, viral interference, and diagnostics. However, the identification and characterization of thermophilic orthologs will expand and unlock the potential of diverse biotechnological applications. Herein, we identified and characterized a …
sa
(code pays fourni par la source)
Accès ouvert
2022
article
OpenAlex
Ahmed Mahas, Qiaochu Wang, Tin Maršić, Magdy M. Mahfouz
Cell-free biosensors can detect various molecules, thus promising to transform the landscape of diagnostics. Here, we developed a simple, rapid, sensitive, and field-deployable small-molecule detection platform based on allosteric transcription factor (aTF)-regulated expression of a clustered regularly interspaced short palindromic repeats (CRISPR) …
sa
(code pays fourni par la source)
Accès ouvert
2022
article
OpenAlex
Rashid Aman, Tin Maršić, Gundra Sivakrishna Rao, Ahmed Mahas et autres
Rapid, specific, and sensitive detection platforms are prerequisites for early pathogen detection to efficiently contain and control the spread of contagious diseases. Robust and portable point-of-care (POC) methods are indispensable for mass screening of SARS-CoV-2. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated …
sa
(code pays fourni par la source)