Akihiro Suto

Nikon Advanced Microscopy - Confocal Reflection Label-Free Imaging v1

Akihiro Suto

Confocal reflection microscopy captures laser light reflected from samples, and allows structures to be visualized without staining or autofluorescence. It can be used to study, for example, biomaterials, cell-growth surfaces, beads within cells, or structures such as blood vessels. Confocal reflection microscopy …

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Nikon Automated Microscopy: Calcium Imaging of cultured cells with a single wavelength calcium indicator (e.g. Fluo-4) v1

Akihiro Suto

This JOB was created in NIS-Elements version 6.10.01, and is designed to image live monolayer-cultured cells (e.g. Cardiomyocytes) stained with a calcium indicator dye such as Fluo-4 using a wide-field microscopy. The JOB will take multiple timelapse recordings at high framerate from …

A macro-transection model of brain trauma for neuromaterial testing with functional electrophysiological readouts

Jessica Patricia Wiseman, Raja Haseeb Basit, Akihiro Suto, Sagnik Middya et autres

JOURNAL/nrgr/04.03/01300535-202512000-00024/figure1/v/2025-01-31T122243Z/r/image-tiff Functional recovery in penetrating neurological injury is hampered by a lack of clinical regenerative therapies. Biomaterial therapies show promise as medical materials for neural repair through immunomodulation, structural support, and delivery of therapeutic biomolecules. However, a lack of facile and pathology-mimetic …

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Nikon Automated Microscopy: Label-free timelapse imaging in well plates with Volume Contrast v1

Akihiro Suto

Brightfield imaging is a label‑free and gentle method for observing cellular growth. However, robust segmentation of cell boundaries in brightfield images remains challenging, particularly when combining brightfield with high‑resolution fluorescence microscopy techniques such as confocal imaging. While methods like differential interference contrast …