Nikon Automated Microscopy: Label-free timelapse imaging in well plates with Volume Contrast v1

Le résumé fourni par la source

Brightfield imaging is a label‑free and gentle method for observing cellular growth. However, robust segmentation of cell boundaries in brightfield images remains challenging, particularly when combining brightfield with high‑resolution fluorescence microscopy techniques such as confocal imaging. While methods like differential interference contrast (DIC) and phase contrast can improve visibility of cell boundaries, they introduce optical components and contrast mechanisms that can degrade fluorescence image quality, making them less suitable when high‑quality fluorescence imaging is required. Nikon Volume Contrast allows the conversion of a small z-stack in brightfield into a fluorescence-like image which highlights the cell cytoplasm, without the addition of any brightfield contrast techniques. This allows for segmentation of the cytoplasmic compartment of a cell, and may be used to replace cytoplasmic or membrane dyes, thus freeing up an extra fluorescence channel. This protocol incorporates a workflow for timelapse imaging in wellplates with volume contrast for live imaging within monolayer cultured cells.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.