Johan E. de Vries

Two novel haemoglobin variants that affect haemoglobin A1c measurement by ion-exchange chromatography

Michael L Bots, An K. Stroobants, Barend Delzenne, Maarten R. Soeters et autres

BACKGROUND: Haemoglobin (Hb) variants are well-known factors interfering with accurate HbA1c testing. This report describes two novel Hb variants leading to inappropriate quantification of HbA1c by ion-exchange chromatography. METHODS: Glycated forms of novel Hb variants were recognised in the blood of two …

nl (code pays fourni par la source)

Tacrolimus pharmacokinetics and pharmacogenetics: influence of adenosine triphosphate‐binding cassette B1 (ABCB1) and cytochrome (CYP) 3A polymorphisms

Robert A.M. Op den Buijsch, Maarten H. L. Christiaans, Leo M. Stolk, Johan E. de Vries et autres

Tacrolimus, an immunosuppressant used after organ transplantation, has a narrow therapeutic range and its pharmacokinetic variability complicates its daily dose assessment. P-glycoprotein (P-gp), encoded by the adenosine triphosphate-binding cassette B1 (ABCB1) and the cytochrome (CYP) 3A4 and 3A5 enzymes appears to play …

nl, de (code pays fourni par la source)

Rapid Genotyping of the OATP1B1 Polymorphisms A388G and T521C with Real-Time PCR FRET Assays

Robert A.M. Op den Buijsch, Otto Bekers, Petal A H M Wijnen, Marja P. van Dieijen‐Visser et autres

The polymorphisms (OATP)1B1 A388G and T521C of the solute carrier organic anion-transporter family member 1B1 gene (SLCO1B1), previously known as OATP-C, have potential impacts on drug metabolism. In order to establish a fast and consistent assay for these polymorphisms, rapid speed polymerase …

nl (code pays fourni par la source)

Capillary electrophoretic detection in apolipoprotein E genotyping

Otto Bekers, Robert A.M. Op den Buijsch, Johan E. de Vries, Petal A H M Wijnen et autres

We describe the application of capillary electrophoresis to detect DNA fragments, obtained after amplifying a part of the apolipoprotein E (apoE) gene with polymerase chain reaction (PCR). Compared to conventional agarose slab gel electrophoresis (AGE), CE appears the method of choice with …

nl (code pays fourni par la source)

PCR on Cell Lysates Obtained from Whole Blood Circumvents DNA Isolation

Johan E. de Vries, Petal A H M Wijnen, Karly Hamulyák, Marja P. van Dieijen‐Visser et autres

Current methods for PCR use purified genomic DNA, isolated mostly from leukocytes. To avoid time-consuming, and therefore expensive, procedures to purify genomic DNA, methods have been developed that use whole blood or buffy coats for PCR (1)(2)(3)(4)(5). However, extensive washing procedures become …

nl (code pays fourni par la source)

PCR on Cell Lysates Obtained from Whole Blood Cir

Johan E. de Vries, Marja P. van Dieijen‐Visser, Otto Bekers

Long-chain fatty acid-induced changes in gene expression in neonatal cardiac myocytes

Karin A. J. M. van der Lee, Michaël M. Vork, Johan E. de Vries, P. H. M. Willemsen et autres

Long-chain fatty acids are the most important substrates for the heart. In addition, they have been shown to affect signalling pathways and gene expression. To explore the effects of long-chain fatty acids on cardiac gene expression, neonatal rat ventricular myocytes were cultured …

nl (code pays fourni par la source)

Transfected c-Ha-ras oncogene enhances karyotypic instability and integrates predominantly in aberrant chromosomes

Johan E. de Vries, Frank H.A.C. Kornips, Patrick Marx, Fred T. Bosman et autres

nl (code pays fourni par la source)

Human Caco-2 cells transfected with c-Ha-Ras as a model for endocrine differentiation in the large intestine

Adriaan P. de Bruı̈ne, Johan E. de Vries, Winand N.M. Dinjens, Peter T.M. Moerkerk et autres

nl (code pays fourni par la source)