Paola G. Andreis

Primary Tissue Culture of Normal Adult Human Decapsulated Adrenal Cortex: Radioautographic Studies on the Metabolic Effects of ACTH 1-2 4

Ubaldo Armato, Paola G. Andreis, Enrica Draghi, Virgilio Meneghelli

ACTH-deprived primary normal adult human adrenocortical cells on the 23rd day in vitro were found to lack any DNA synthetic and proliferative activity, but incorporated uridine-3H and 1-leucine-3H actively. In cortical cells of the same age treated for 2 and 7 days …

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Evidence for a paracrine role of endogenous adrenomedullary galanin in the regulation of glucocorticoid secretion in the rat adrenal gland

Paola G. Andreis, Cinzia Tortorella, Agnieska Ziolkowska, Raffaella Spinazzi et autres

Previous investigations have shown that rat adrenocortical cells are provided with galanin receptors, and galanin stimulates glucocorticoid secretion from dispersed cells. The present study aimed to clarify the possible role of galanin in the physiological regulation of rat adrenal secretory activity. Reverse …

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Galanin enhances corticosterone secretion from dispersed rat adrenocortical cells through the activation of GAL-R1 and GAL-R2 receptors coupled to the adenylate cyclase-dependent signaling cascade

Paola G. Andreis, Ludwik K. Malendowicz, Piera Rebuffat, Raffaella Spinazzi et autres

Galanin is a regulatory peptide, which acts via three subtypes of receptors, named GAL-R1, GAL-R2 and GAL-R3. Reverse transcription-polymerase chain reaction demonstrated the expression of GAL-R1 and GAL-R2, but not GAL-R3 mRNAs in dispersed rat adrenal zona fasciculata-reticularis (inner) cells. The immuno-blockade …

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In vitro culture on Matrigel favors the long-term maintenance of rat zona glomerulosa-cell differentiated phenotype

Raffaella Spinazzi, Lucía Petrelli, Diego Guidolin, Gianni Carraro et autres

Zona glomerulosa (ZG) cells cultured on plastic within few days dedifferentiate losing their capacity to secrete aldosterone (ALDO) in appreciable amounts. Evidence indicates that extracellular matrix modulates the secretory behavior of adrenocortical cells cultured in vitro. Hence, we compared the morphology and …

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An image analysis of the spatial distribution of perivascular mast cells in human melanoma

Diego Guidolin, Enrico Crivellato, Beatrice Nico, Paola G. Andreis et autres

A mutual spatial and functional relationship occurs between mast cells (MCs) and endothelial cells and the density of MCs is highly correlated with the extent of tumor angiogenesis. The aim of this study was to investigate the pattern of MCs around the …

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Orexins in the Regulation of the Hypothalamic-Pituitary-Adrenal Axis

Raffaella Spinazzi, Paola G. Andreis, Gian Paolo Rossi, Gastone Giovanni Nussdorfer

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Neuropeptides B and W enhance the growth of human adrenocortical carcinoma-derived NCI-H295 cells by exerting MAPK p42/p44-mediated proliferogenic and antiapoptotic effects

Paola G. Andreis, Marcin Ruciński, Giuliano Neri, Maria Teresa Conconi et autres

Neuropeptides B and W (NPB and NPW) are endogenous ligands of two G protein-coupled receptors, named GPR7 and GPR8. GPR7 and GPR8 are expressed in the adrenal cortex, and there is evidence that NPB and NPW stimulate glucocorticoid secretion from human adrenocortical …

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Adrenomedullin and vascular endothelium growth factor genes are overexpressed in the regenerating rat adrenal cortex, and AM and VEGF reciprocally enhance their mRNA expression in cultured rat adrenocortical cells

Giovanna Albertin, Marcin Ruciński, Gianni Carraro, Myriam Forneris et autres

Adrenomedullin (AM) is an endogenous regulatory peptide, which exerts growth promoting and neoangiogenic actions in several normal and neoplastic tissues. Evidence has been provided that AM and the pro-angiogenic peptide vascular endothelium growth factor (VEGF) are expressed in the adrenal gland, and …

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Differential expression and function of beacon in the rat adrenal cortex and medulla

Marcin Ruciński, Paola G. Andreis, Agnieszka Ziółkowska, Gastone Giovanni Nussdorfer et autres

Beacon gene is overexpressed in obese rats, and beacon was found to stimulate food intake. Evidence has been recently provided that beacon is also expressed in the endocrine glands of normal rats, including adrenal cortex, of which it appears to regulate secretory …

Orexins modulate the growth of cultured rat adrenocortical cells, acting through type 1 and type 2 receptors coupled to the MAPK p42/p44- and p38-dependent cascades

Raffaella Spinazzi, Agnieszka Ziółkowska, Giuliano Neri, Magdalena Nowak et autres

Orexin A and B are hypothalamic peptides that act through two subtypes of receptors named OX1-R and OX2-R. The OX1-R almost exclusively binds orexin-A, whereas OX2-R is non-selective for both orexins. We previously found that rat adrenocortical cells express both orexin-receptor subtypes, …

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Neuropeptide-Y and Y-receptors in the autocrine-paracrine regulation of adrenal gland under physiological and pathophysiological conditions (Review)

Raffaella Spinazzi, Paola G. Andreis, Gastone Giovanni Nussdorfer

Neuropeptide-Y (NPY) is a 36-amino acid peptide, which belongs, along with peptide YY (PYY), to the pancreatic polypeptide (PP) family. The members of this family of peptides act via G protein-coupled receptors (Rs), six subtypes of which (from Y1- to Y6-R) have …

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Up-regulation of the adrenomedullin system mediates hypotension and hypoaldosteronism induced by simulated microgravity

Paola G. Andreis, Gian Paolo Rossi, Sergio Bova, Giuliano Neri et autres

We recently demonstrated that prolonged simulated microgravity (SMG) induced hypotension and hypoaldosteronism in rats, and gathered preliminary evidence for an involvement of circulating adrenomedullin (AM). Thus, we aimed to investigate whether short-term SMG elicits the same effects, and whether up-regulation of adrenal …

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