Shawn Mishra

Driving novel endpoints and study designs in amyotrophic lateral sclerosis: closer examination of the ALSFRS-R subdomains and a new definition of fast and slow progressors

Robert Ellis, W. Benjamin Nowell, Matthew F. Wipperman, Jiangnan Lyu et autres

Abstract Amyotrophic lateral sclerosis (ALS) is a motor neuron disease that leads to significant morbidity and mortality, but with substantial variability in the rate of progression [1,2]. A common primary outcome measure used in ALS clinical studies is the ALSFRS-R. Change in …

us (code pays fourni par la source)

Image guided construction of a common coordinate framework for spatial transcriptome data

Peter Lais, Shawn Mishra, Kun Xiong, Gurinder S. Atwal et autres

Spatial transcriptomics is a powerful technology for high-resolution mapping of gene expression in tissue samples, enabling a molecular level understanding of tissue architecture. The acquisition entails dissecting and profiling micron-thick tissue slices, with multiple slices often needed for a comprehensive study. However, …

us (code pays fourni par la source)

Image guided construction of a common coordinate framework for spatial transcriptome data

Peter Lais, Shawn Mishra, Kun Xiong, Gurinder S. Atwal et autres

Abstract Spatial transcriptomics is a powerful technology for high-resolution mapping of gene expression in tissue samples, enabling a molecular level understanding of tissue architecture. The acquisition entails dissecting and profiling micron-thick tissue slices, with multiple slices often needed for a comprehensive study. …

us (code pays fourni par la source)

Age-related gene expression signatures from limb skeletal muscles and the diaphragm in mice and rats reveal common and species-specific changes

Tea Shavlakadze, Kun Xiong, Shawn Mishra, Corissa McEwen et autres

BACKGROUND: As a result of aging, skeletal muscle undergoes atrophy and a decrease in function. This age-related skeletal muscle weakness is known as "sarcopenia". Sarcopenia is part of the frailty observed in humans. In order to discover treatments for sarcopenia, it is …

us (code pays fourni par la source)

Population-scale analysis of common and rare genetic variation associated with hearing loss in adults

Kavita Praveen, Lee Dobbyn, Lauren Gurski, Ariane Ayer et autres

Abstract To better understand the genetics of hearing loss, we performed a genome-wide association meta-analysis with 125,749 cases and 469,497 controls across five cohorts. We identified 53/c loci affecting hearing loss risk, including common coding variants in COL9A3 and TMPRSS3 . Through …

us (code pays fourni par la source)

A Microfluidic Eye Facsimile System to Examine the Migration of Stem-like Cells

Stephen Ryan Mut, Shawn Mishra, Maribel Vázquez

Millions of adults are affected by progressive vision loss worldwide. The rising incidence of retinal diseases can be attributed to damage or degeneration of neurons that convert light into electrical signals for vision. Contemporary cell replacement therapies have transplanted stem and progenitor-like …

us (code pays fourni par la source)

Population-scale analysis of common and rare genetic variation associated with hearing loss in adults

Kavita Praveen, Lee Dobbyn, Lauren Gurski, Ariane Ayer et autres

ABSTRACT Understanding the genetic underpinnings of disabling hearing loss, which affects ∼466 million people worldwide, can provide avenues for new therapeutic target development. We performed a genome-wide association meta-analysis of hearing loss with 125,749 cases and 469,497 controls across five cohorts, including …

us, se (code pays fourni par la source)

A novel electro-chemotactic approach to impact the directional migration of transplantable retinal progenitor cells

Shawn Mishra, Juan S. Peña, Stephen Redenti, Maribel Vázquez

es, us (code pays fourni par la source)

Controlled Migration of Retinal Progenitor Cells Within Electro-Chemotactic Fields

Shawn Mishra

Vision loss in retinal degenerative diseases is overwhelmingly attributed to damage and death of retinal photoreceptor cells. Studies in mouse retina have suggested that transplantation of isolated post-natal or stem cell-derived retinal progenitor cells (RPCs) to replace apoptotic or damaged photoreceptors may …

Collective adhesion and displacement of retinal progenitor cells upon extracellular matrix substrates of transplantable biomaterials

Ankush Thakur, Shawn Mishra, Juan S. Peña, Jing Zhou et autres

Strategies to replace retinal photoreceptors lost to damage or disease rely upon the migration of replacement cells transplanted into sub-retinal spaces. A significant obstacle to the advancement of cell transplantation for retinal repair is the limited migration of transplanted cells into host …

us (code pays fourni par la source)

A Gal-MµS Device to Evaluate Cell Migratory Response to Combined Galvano-Chemotactic Fields

Shawn Mishra, Maribel Vázquez

Electric fields have been studied extensively in biomedical engineering (BME) for numerous regenerative therapies. Recent studies have begun to examine the biological effects of electric fields in combination with other environmental cues, such as tissue-engineered extracellular matrices (ECM), chemical gradient profiles, and …

us (code pays fourni par la source)

Predicted molecular signaling guiding photoreceptor cell migration following transplantation into damaged retina

Uchenna Unachukwu, Alice Warren, Ze Li, Shawn Mishra et autres

To replace photoreceptors lost to disease or trauma and restore vision, laboratories around the world are investigating photoreceptor replacement strategies using subretinal transplantation of photoreceptor precursor cells (PPCs) and retinal progenitor cells (RPCs). Significant obstacles to advancement of photoreceptor cell-replacement include low …

us (code pays fourni par la source)