He Ying

Age-dependent prognostic value of biological age for metastasis and survival in gastrointestinal cancer

Jinping Zheng, Chunhua Song, Hongxia Xu, Zengqing Guo et autres

Chronological age is a recognized cancer risk factor but does not capture inter-individual differences in systemic aging. Biological age, reflecting cumulative functional decline, may better predict cancer outcomes. This study examined associations between biological age, disease characteristics, metastasis, and survival in gastrointestinal …

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Determination of Spermidine and other 10 Biogenic Amines in Radix astragali (Huang qi) by HPLC-DAD with Precolumn Derivatization

Sun Yan Ni, Yun Ai, Wang Jin Rui, He Ying et autres

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Protective effect of sipunculusnudus polysaccharide on HUVEC cell damage induced by 137 Cs γ-ray

Chunli Gao, Qingrong Wang, Chen Xianrong, Shuang Bai et autres

Objective To investigate the protective effect of sipunculusnudus polysaccharide (SNP) on HUVEC cell damage induced by ionizing radiation. Methods The survival rate of HUVEC cells was detected by CCK-8, after irradiation with 137Cs γ-rays and pre-treatment with SNP, and optimal absorption dose …

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miR-628, a microRNA that is induced by Toll-like receptor stimulation, regulates porcine innate immune responses

Jun He, He Ying, Chen Dai-wen, Bing Cai Yu et autres

Mammalian innate and acquired immune responses involve a coordinated, sequential, and self limiting sequence of events controlled by positive and negative regulatory mechanism. MicroRNAs have been implicated as a negative regulator for diverse biological events including immune responses. However, the involvement of …

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Cloning and expression of pyruvate dehydrogenase E1-α subunit gene (pdha) in Mycoplasma ovipneumoniae and its immunologic activity evaluation.

Jian Xu, Yuefeng Chu, Gao Peng-cheng, Ping Zhao et autres

Effect of mCD99L2 gene silencing on the transformation of mice lymphoma cell line A20 into H/RS-like cells

He Ying, Shen Lijia, Zuguo Li, Xie Wei-bing et autres

Objective To investigate the effects of mCD99L2 gene silencing on the transformation of mice B lymphoma cell line A20 to H/RS-like cells. Methods Recombinant plasmid LV-mCD99L2 was transfected into A20 cells which endogenetically express mCD99L2. The stably expressed cell line was selected …

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Apoptosis of human colon carcinoma cells induced by dexamethasone in vitro

He Ying

OBJECTIVE:To examine the expression of the glucocorticoid receptor and the effect on proliferation and apoptosis induced by Dexamethasone in human colon carcinoma cell lines Lovo, HCT-116,HT-29 and SW-480 in vitro. METHODS: GR protein was determined by immunohistochemistry and GR mRNA by RT-PCR. …

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Construction of recombinant plasmid pEGFP-anxA5 and its expression in Hela cells

Xin Li, Fang Wang, He Ying

Objective:To construct the recombinant plasmid pEGFP-anxA5 capable be expressed in mammalian cells.Methods:Annexin A5 gene containing Xho I and Barn HI endoenzyme sites were obtained by PCR.Double enzyme digestion was conducted for green fluorescent protein vector pEGFP-N1 and PCR product of annexin A5 …

Establishment of CHO cell line stably overexpressing human wild type DNA polymerase beta gene and its biological characteristics

Min Li, He Ying, Guo-qiang Zhao, Dong Ziming

A recombined enhanced green fluorescent protein(GFP) vector pEGFP-AC3 carrying wide type DNA pol βgene was transfected into CHO cells by lipotransfection,and the stable transfectants were screened by G418.Stable overexpression of pol β in CHO was confirmed by RT-PCR,and its biological characteristics were …

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