Apoptosis of human colon carcinoma cells induced by dexamethasone in vitro

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OBJECTIVE:To examine the expression of the glucocorticoid receptor and the effect on proliferation and apoptosis induced by Dexamethasone in human colon carcinoma cell lines Lovo, HCT-116,HT-29 and SW-480 in vitro. METHODS: GR protein was determined by immunohistochemistry and GR mRNA by RT-PCR. Proliferation and apoptosis were detected by MTT, DNA Ladder method and flow cytometer. RESULTS: The glucocorticoid receptor was determined in Lovo and HCT-116, not in HT-29 and SW-480. Dexamethasone had an inhibitory effect on the four human colon cancer cell lines, respectively, and Lovo and HCT-116 cells were among the most sensitive. And the characteristic apoptotic DNA Ladders of Lovo were confirmed by DNA Ladder method. Apoptosis cusps were investigated by flow cytometer in Lovo and HCT-116, the rates of apoptosis [Lovo(34.8±1.9)% and HCT-116(33.6±1.4)%] were higher than those [Lovo(2.9±0.4)% and HCT-116(6.4±1.3)%] in the control groups without Dex. CONCLUSION: The results demonstrate expression of the glucocorticoid receptor in Lovo and HCT-116. Inhibiton of proliferation and apoptosis occur on Lovo and HCT-116 cells induced by dexamethasone in vitro, which may have some relationship with glucocorticoid receptor.

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