Ye Eun Oh

Precise genomic integration of large DNA fragments by donor-directed annealing using prime editing

Hojun Jung, Bada Jeong, Yong-Woo Kim, Chanju Jung et autres

Replacing large-scale fragments in human cells remains a substantial challenge. Here, we present a programmable gene replacement tool, named prime assembly (PA), which adapts prime editors to produce one or two pairs of 3'-flaps on both the genome and donor DNA. These …

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Engineered ADARs enable precision A-to-G base editing of DNA

Hyeon Woo Im, Bada Jeong, Yeji Lee, Ye Eun Oh et autres

Adenine base editors (ABEs), which enable A•T-to-G•C base editing, have emerged as a powerful tool with potential therapeutic applications. However, conventional ABEs suffer from bystander nucleotide conversions, limiting their utility for precise editing. Here we present a single-nucleotide resolution ABE (snuABE) created …

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AI-generated MLH1 small binder improves prime editing efficiency

Ju-Chan Park, Heesoo Uhm, Yong Woo Kim, Ye Eun Oh et autres

The prime editing (PE) system consists of a Cas9 nickase fused to a reverse transcriptase, which introduces precise edits into the target genomic region guided by a PE guide RNA. However, PE efficiency is limited by mismatch repair. To overcome this limitation, …

Éthiopie, kr, jp (code pays fourni par la source)

AI-generated small binder improves prime editing

Ju-Chan Park, Heesoo Uhm, Yong Woo Kim, Ye Eun Oh et autres

Abstract The prime editing 2 (PE2) system comprises a nickase Cas9 fused to a reverse transcriptase utilizing a prime editing guide RNA (pegRNA) to introduce desired mutations at target genomic sites. However, the PE efficiency is limited by mismatch repair (MMR) that …

Éthiopie, kr (code pays fourni par la source)

Ai-Generated Small Binder Improves Prime Editing

Ju-Chan Park, Heesoo Uhm, Yong Woo Kim, Ye Eun Oh et autres

P-91 IGFBP-3 in cellular senescence; molecular and clinical implications

Amanda Garza, Ye Eun Oh