JANOS K. LANYI

Expression of Xanthorhodopsin in Escherichia coli

Lada E. Petrovskaya, Evgeniy P. Lukashev, Ekaterina N. Lyukmanova, Mikhail A. Shulepko et autres

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Proton transfer reactions in donor site mutants of ESR, a retinal protein from Exiguobacterium sibiricum

Lada E. Petrovskaya, Evgeniy P. Lukashev, Sergey A. Siletsky, Eleonora S. Imasheva et autres

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Bioenergetics and Transport in Extreme Halophiles

JANOS K. LANYI

The import and export of ions and metabolites across cellular and organelle membranes are now understood in terms of Peter Mitchell’s chemiosmotic theory. In its broad outlines the latter states that the translocations across the essentially impermeable barrier of the lipid bilayer …

Bioenergetics of halophiles

JANOS K. LANYI, Basic Energy Sciences (BES) (SC-22) USDOE Office of Science (SC), Sergei P. Balashov

In the last grant period we explored the Na+ binding site of the recently discovered light-driven sodium ion pump. The rationale was that comparison of this novel system to the similar proton pumps and chloride ion pumps would reveal the amazingly (and …

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ESR — A retinal protein with unusual properties from Exiguobacterium sibiricum

Lada E. Petrovskaya, Sergei P. Balashov, Eugene P. Lukashev, Eleonora S. Imasheva et autres

This review covers the properties of a retinal protein (ESR) from the psychrotrophic bacterium Exiguobacterium sibiricum that functions as a light-driven proton pump. The presence of a lysine residue at the position corresponding to intramolecular proton donor for the Schiff base represents …

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Light-Driven Na+ Pump from Gillisia limnaea : A High-Affinity Na+ Binding Site Is Formed Transiently in the Photocycle

Sergei P. Balashov, Eleonora S. Imasheva, Andrei K. Dioumaev, Jennifer M. Wang et autres

A group of microbial retinal proteins most closely related to the proton pump xanthorhodopsin has a novel sequence motif and a novel function. Instead of, or in addition to, proton transport, they perform light-driven sodium ion transport, as reported for one representative …

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Directed evolution of a far-red fluorescent rhodopsin

R. Scott McIsaac, Martin K. M. Engqvist, Timothy M. Wannier, Adam Rosenthal et autres

Microbial rhodopsins are a diverse group of photoactive transmembrane proteins found in all three domains of life. A member of this protein family, Archaerhodopsin-3 (Arch) of halobacterium Halorubrum sodomense, was recently shown to function as a fluorescent indicator of membrane potential when …

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Nature’s toolkit for microbial rhodopsin ion pumps

Oded Béjà, JANOS K. LANYI

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans the biological, physical, and social sciences.

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Photocycle of Exiguobacterium sibiricum Rhodopsin Characterized by Low-Temperature Trapping in the IR and Time-Resolved Studies in the Visible

Andrei K. Dioumaev, Lada E. Petrovskaya, Jennifer M. Wang, Sergei P. Balashov et autres

The photocycle of the retinal protein from Exiguobacterium sibiricum, which differs from bacteriorhodopsin in both its primary donor and acceptor, is characterized by visible and infrared spectroscopy. At pH above pKa ~6.5, we find a bacteriorhodopsin-like photocycle, which originates from excitation of …

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Breaking the Carboxyl Rule

Sergei P. Balashov, Lada E. Petrovskaya, Eleonora S. Imasheva, Evgeniy P. Lukashev et autres

A lysine instead of the usual carboxyl group is in place of the internal proton donor to the retinal Schiff base in the light-driven proton pump of Exiguobacterium sibiricum (ESR). The involvement of this lysine in proton transfer is indicated by the …

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Interaction of Antenna Carotenoid and Retinal in the Light-Driven Pumps of Salinibacter Ruber and Gloeobacter Violaceus

JANOS K. LANYI, Sergei P. Balashov, Ella S. Imasheva, Hartmut Luecke et autres

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Carotenoid Antenna Binding and Function in Retinal Proteins

JANOS K. LANYI, Sergei P. Balashov

Abstract : Xanthorhodopsin, a proton pump from the eubacterium Salinibacter ruber, is a unique dual chromophore system that contains, in addition to retinal, the carotenoid salinixanthin as a light-harvesting antenna. The key factors affecting binding and function of the antenna were established …