Dirk-Jan E. Opstelten

Genetically engineered allergens formulated with thermosensitive hydrogel enable safe and fast up-dosing in allergen immunotherapy and induce a strong protective immune response

Juha Rouvinen, Jaana Haka, Silja Halme, Pekka Mattila et autres

Subcutaneous Allergen Immunotherapy in Adults Allergic to House Dust Mites: A Phase 3 Randomized Controlled Trial

Claus Bachert, Hans Hoogeveen, Rimko ten Have, Donghui Yu et autres

BACKGROUND: Subcutaneous allergen immunotherapy (SCIT) has a longstanding history as a safe and effective treatment. Nevertheless, to meet the European Medicines Agency's regulatory requirements for continued market authorization, its efficacy and safety must be confirmed in a pivotal Phase III trial. OBJECTIVE: …

de, nl, pl (code pays fourni par la source)

Development Of A Subcutaneous Immunotherapy (SCIT) With A Modified Peanut Extract Formulation For The Treatment Of Peanut Allergy

Andrey S. Larionov, Ioanna Stavrakaki, Edwin Kim, Scott Howard Sicherer et autres

nl, us, ca (code pays fourni par la source)

SCIT-treatment With a Chemically Modified, Aluminum Hydroxide Adsorbed Peanut Extract (HAL-MPE1) Was Generally Safe And Well Tolerated And Showed Immunological Changes In Peanut Allergic Patients

Carsten Bindslev‐Jensen, Pieter‐Jan de Kam, Esther van Twuijver, Diderik Boot et autres

dk, nl (code pays fourni par la source)

Birch Pollen Immunotherapy in Mice: Inhibition of Th2 Inflammation Is Not Sufficient to Decrease Airway Hyper-Reactivity

Leonie S. van Rijt, L. Utsch Mendes Gouveia, Adrian Logiantara, Derya Canbaz et autres

BACKGROUND: Suppression of Th2 cytokine production by allergen-specific Th2 cells is considered to be critical for the suppression of allergic symptoms by subcutaneous immunotherapy. The aim of this study was to develop a mouse model for birch pollen (BP) immunotherapy to elucidate …

nl (code pays fourni par la source)

Envelope Glycoprotein Interactions in Coronavirus Assembly

Dirk-Jan E. Opstelten, Martin J. B. Raamsman, Karin Wolfs, Marian C. Horzinek et autres

Abstract. Coronaviruses are assembled by budding into smooth membranes of the intermediate ERto-Golgi compartment. We have studied the association of the viral membrane glycoproteins M and S in the formation of the virion envelope. Using coimmunoprecipitation analysis we demonstrated that the M …

FAST: towards safe and effective subcutaneous immunotherapy of persistent life‐threatening food allergies

Laurian Zuidmeer, Montserrat Fernández‐Rivas, Lars Kaergaard Poulsen, Angela Neubauer et autres

The FAST project (Food Allergy Specific Immunotherapy) aims at the development of safe and effective treatment of food allergies, targeting prevalent, persistent and severe allergy to fish and peach. Classical allergen-specific immunotherapy (SIT), using subcutaneous injections with aqueous food extracts may be …

nl, es, dk, at, ca, is, it, gb, pl, fr, gr (code pays fourni par la source)

Effect of modification of peanut conglutin on IgE and T cell reactivity in adults with peanut allergy

Els van Hoffen, Hanneke P. M. van der Kleij, S. Jäger, Edward F. Knol et autres

In peanut-allergic patients, specific immunotherapy using peanut extract has been shown to be associated with significant side effects. Chemically modified allergens may provide a safer alternative. Purified native peanut conglutin, containing Ara h2 and Ara h6, was chemically modified by reduction and …

nl (code pays fourni par la source)

Vitamin D3 targets epidermal and dermal dendritic cells for induction of distinct regulatory T cells

Angelic M.G. van der Aar, Darya S. Sibiryak, Ghaith Bakdash, Toni M.M. van Capel et autres

nl (code pays fourni par la source)

PER.C6® Cells for the Manufacture of Biopharmaceutical Proteins

Chris Yallop, John Crowley, Johanne Côté, Kirsten Hegmans‐Brouwer et autres

This chapter contains sections titled: Introduction Generation of PER.C6 Cells PER.C6 Cells for the Manufacture of Recombinant Proteins Fed-batch Process Development Operation of PER.C6 Cells in Continuous Perfusion Characterization of Antibodies Produced by PER.C6 Cells Conclusion

High‐Level Expression of Recombinant IgG in the Human Cell Line PER.C6

David Jones, Nathalie Kroos, Regina Anema, Bart van Montfort et autres

The number of therapeutic monoclonal antibodies in production is expected to rise rapidly in the next few years. As a result, there is much focus on the optimization of antibody expression platforms. Several issues are important including the speed of transition from …

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Efficient Generation and Amplification of High-Capacity Adeno-Associated Virus/Adenovirus Hybrid Vectors

Manuel A.F.V. Gonçalves, Ietje van der Velde, Josephine M. Janssen, Bram Maassen et autres

Effective gene therapy is dependent on safe gene delivery vehicles that can achieve efficient transduction and sustained transgene expression. We are developing a hybrid viral vector system that combines in a single particle the large cloning capacity and efficient cell cycle-independent nuclear …

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