J. R. N. McLean

Prediction of radiosensitivity by measurement of X-ray induced apoptosis in human blood using the comet assay.

N. Kizilian-Martel, Ruth C. Wilkins, J. R. N. McLean, Shawn Christopher Malone et autres

BACKGROUND: The aim of this work was to determine whether levels of radiation-induced apoptosis in human peripheral leukocytes could be used as a predictor of radiosensitivity. MATERIALS AND METHODS: Peripheral blood was obtained from venous blood and exposed to 0-3 Gy of …

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A 60 Hz magnetic field does not affect the incidence of squamous cell carcinomas in SENCAR mice

J. R. N. McLean, A. Thansandote, James P. McNamee, Leander Tryphonas et autres

Two groups of SENCAR mice were treated with a single dose of carcinogen and then, for 23 weeks, with a chemical tumor promoter to induce skin tumors. During this period, one group was coexposed to a 2 mT power frequency (60 Hz) …

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Prompt and Delayed Nonsteroidal Anti-inflammatory Drug-photoinduced DNA Damage in Peripheral Blood Mononuclear Cells Measured with the Comet Assay ¶

Anne L. Vinette, James P. McNamee, Pascale V. Bellier, J. R. N. McLean et autres

Nonsteroidal anti-inflammatory drug (NSAID)-photoinduced DNA damage in human peripheral blood mononuclear cells measured using the alkaline comet assay is presented. Whereas Tiaprofenic Acid-photoinduced DNA damage was promptly induced (i.e. observed at relatively low radiation doses), Ketoprofen-photoinduced DNA damage was delayed. This prompt …

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Automated measurements of tails in the single cell gel electrophoresis assay

Jean-François Rivest, Mingxiang Tang, J. R. N. McLean, Francis Johnson

This paper presents a method to automate a new technique, which has been developed to detect primary DNA damage of individual cells. It is based on single cell gel electrophoresis: the cells are placed in a gel, and then are prepared in …

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Silver-stained "comets" for the detection of radiation-induced apoptosis: automated assay

D. Gibbons, Faye M. Johnson, Ruth C. Wilkins, N. Kizilian et autres

This paper reviews the development of a bioassay that uses apoptosis (a form of cell death) to directly measure the response of cells to a physical or chemical insult. The assay described here is in mid-phase of development and has been applied …

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DNA Damage Detection Technique Applying Time-Resolved Fluorescence Measurements

Gonzalo Cosa, Anne L. Vinette, J. R. N. McLean, J. C. Scaiano

A novel DNA damage detection technique based on the characteristic fluorescence lifetimes exhibited by Pico-Green-single-stranded DNA and -double-stranded DNA complexes is employed to establish the damage produced on DNA isolated from sheep white blood cells following gamma radiation. This technique, which incorporates …

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Analysis of radiation‐induced apoptosis in human lymphocytes: Flow cytometry using Annexin V and propidium iodide versus the neutral comet assay

Ruth C. Wilkins, B. C. Kutzner, Mai Thy Truong, Jaime Sanchez-Dardon et autres

BACKGROUND: The neutral comet assay was devised to measure double-stranded DNA breaks, but it has also been used to measure apoptosis based on its characteristic DNA fragmentation patterns. There is still uncertainty about the reliability of this method. By comparing the comet …

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DNA Damage Caused by Photoexcited Ketoprofen Measured by the Comet Assay

Anne L. Vinette, J. R. N. McLean, James P. McNamee, P. Belier et autres

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DNA damage and apoptosis in the immature mouse cerebellum after acute exposure to a 1 mT, 60 Hz magnetic field

James P. McNamee, Pascale V. Bellier, J. R. N. McLean, Leonora Marro et autres

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The effect of the ratio of CD4 + to CD8 + T-cells on radiation-induced apoptosis in human lymphocyte subpopulations

Ruth C. Wilkins, B. C. Kutzner, Mai Thy Truong, J. R. N. McLean

PURPOSE: Apoptosis occurs spontaneously in cultured human peripheral blood lymphocytes but is enhanced by exposure to ionizing radiation. Subpopulations of lymphocytes are known to have varying radiosensitivities to radiation-induced apoptosis. The purpose of this study was to examine the radiation-induced apoptotic response …

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Differential apoptotic response to ionizing radiation in subpopulations of human white blood cells

Ruth C. Wilkins, David J.P. Wilkinson, H.P. Maharaj, Pascale V. Bellier et autres

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DIFFERENTIAL RATES OF CYTOKINE PRODUCTION AND APOPTOSIS IN VENIPUNCTURE AND FINGER-STAB DERIVED BLOOD CULTURES

James P. McNamee, Pascale V. Bellier, J. R. N. McLean

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