Alexander M. Buko

CBD Disrupts Malme-3M Cell Metabolism via Glycolytic Shift and Redox Imbalance

Laura M. Shelton, Yiling Xu, Hans K. Ghayee, Alexander M. Buko

BACKGROUND: Accumulating evidence suggests that cannabidiol (CBD) exerts variable effects on cancer cells that influence cellular activity, including growth. While anecdotal evidence abounds, mechanistic studies have lagged. METHODS: Malme-3M cells derived from melanoma and less-aggressive BJ fibroblast cells were incubated with CBD. …

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CBD Disrupts Malme-3M Cell Metabolism via Glycolytic Shift and Redox Imbalance

Laura M. Shelton, Yiling Xu, Ghayee Hans, Alexander M. Buko

Background: Accumulating evidence suggests that cannabidiol (CBD) exerts variable effects on cancer cells that influences cellular activity including growth. While anecdotal evidence abounds, mechanistic studies have lagged behind. Methods: CBD treatment on malignant fibroblast (Malme-3M) and benign BJ fibroblast cells was performed. …

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Differentiation of patients with and without prostate cancer using urine 1H NMR metabolomics

Anna‐Laura Hasubek, Xiaoyu Wang, Ella Zhang, Marta Kobus et autres

Prostate cancer (PCa) is one of the most prevalent cancers in men worldwide. For its detection, serum prostate-specific antigen (PSA) screening is commonly used, despite its lack of specificity, high false positive rate, and inability to discriminate indolent from aggressive PCa. Following …

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Metabolomics Approaches Confer a Deeper Biologic Understanding of Kidney-Related Diseases

Alexander M. Buko

Abstract 3510: The metabolomic profile of urine from prostate cancer patients using capillary electrophoresis mass spectrometry (CEMS)

Alexander M. Buko, Leo L. Cheng, Andrew Gustev, Adam S. Feldman

Abstract Background: Prostate cancer (PCa) pathogenesis is influenced by alterations in cellular metabolism. Metabolomics measures these biochemical changes to create global tissue metabolite profiles. Urinary studies are noninvasive and can potentially identify biomarkers for PCa. Objective: We used Capillary Electrophoresis Mass Spectrometry …

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Capillary electrophoresis mass spectrometry based metabolomics

Alexander M. Buko

Capillary electrophoresis–mass spectrometry (CE-MS) is a powerful orthogonal technique capable of filling in gaps in the identification, quantitation and isomeric resolution of many small hydrophilic and charged metabolites. The metabolome is a large complex mixture of molecules for which not one technique …

Identification of a Yellow Impurity in Aged Samples of Aqueous Butamben Suspension: Evidence for the Oxidative Degradation of Poly(ethylene glycol)

Eric J. Ginsburg, Dennis Stephens, Paul R. West, Alexander M. Buko et autres

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Purification of Commercial Coomassie Brilliant Blue R-250 and Characterization of the Chromogenic Fractions

Samar K. Kundu, W. Gerard Robey, Priscilla Nabors, Martin R. Lopez et autres

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HPLC and PDMS analysis of cleavage products aid in the design of small, stable ANP analogues

Edward M. Devine, Alexander M. Buko, Steven Cepa, Steven K. Davidsen et autres

The degradation of a prototypical small analogue of atrial natriuretic peptide (ANP) has been studied using HPLC and mass spectrometric techniques. These studies revealed that removal of the N-terminal amino acid was the primary catabolic event in vitro. Based on this information …

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Letter to the editors

Steven Cepa, Preston Hill, Alexander M. Buko

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Sequence‐specific fragmentation from oligopeptides using plasma‐desorption mass spectrometry

Alexander M. Buko, Virender K. Sarin

252Cf plasma-desorption mass spectrometry (PDMS) has been demonstrated to provide sequence-specific fragmentation for several oligopeptides. The nature of the fragment ions observed is generally similar to that observed using liquid secondary-ion mass spectrometry (LSIMS) and can be observed using less sample than …

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Carbohydrate composition and presence of a fucose-protein linkage in recombinant human pro-urokinase

Elzbieta J. Kentzer, Alexander M. Buko, Gopi Menon, Virender K. Sarin

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