CBD Disrupts Malme-3M Cell Metabolism via Glycolytic Shift and Redox Imbalance

Le résumé fourni par la source

Background: Accumulating evidence suggests that cannabidiol (CBD) exerts variable effects on cancer cells that influences cellular activity including growth. While anecdotal evidence abounds, mechanistic studies have lagged behind. Methods: CBD treatment on malignant fibroblast (Malme-3M) and benign BJ fibroblast cells was performed. LC MS/MS mass spectroscopy was used to measure metabolites from CBD treatment. Results: Data indicate a differential response between malignant fibroblast (Malme-3M) cells and benign fibroblasts (BJ) cells with respect to metabolites critical for primary metabolic function. A significant reduction in TCA metabolites is seen with a corresponding increase in glycolytic output in the Malme-3M cell line while a similar reduction in TCA activity in the fibroblasts appears to differentially activate fatty acid oxidation. ATP is significantly reduced in the Malme-3M cells with a corresponding decrease in metabolites associated with redox maintenance. Conclusions: This is the first metabolomics analyses of both normal and tumor derived cell lines after pre-treatment with CBD. The data suggest that the metabolic perturbation, because of CBD treatment, could reprogram and affect cellular metabolism and redox maintenance.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.