Cheryl Lyons

Common, low-frequency, rare, and ultra-rare coding variants contribute to COVID-19 severity

Chiara Fallerini, Nicola Picchiotti, Margherita Baldassarri, Kristina Zguro et autres

The combined impact of common and rare exonic variants in COVID-19 host genetics is currently insufficiently understood. Here, common and rare variants from whole-exome sequencing data of about 4000 SARS-CoV-2-positive individuals were used to define an interpretable machine-learning model for predicting COVID-19 …

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Relationships between Conceptual Knowledge and Reasoning about Systems: Implications for Fostering Systems Thinking in Secondary Science

Cheryl Lyons

Reasoning about systems is necessary for understanding many modern issues that face society and is important for future scientists and all citizens. Systems thinking may allow students to make connections and identify common themes between seemingly different situations and phenomena, and is …

Quantitative Analysis of Minimal Residual Disease at the Completion of Induction Therapy Predicts Relapse in Children with B-Lineage Acute Lymphoblastic Leukemia in DFCI ALL Consortium Protocol 95-01.

Jianbiao Zhou, Aihong Li, Meredith A. Goldwasser, Hongjun Wang et autres

Abstract Quantitative analysis of minimal residual disease (MRD) is emerging as among the most important predictors of subsequent relapse in childhood ALL. In 381 of 438 (87%) sequential cases of childhood B-lineage ALL enrolled on DFCI Consortium protocol 95-01, we could sequence …

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Molecular Analysis of T-Cell Receptor Gene Rearrangements in Children with T-Cell Acute Lymphoblast Leukemia on DFCI ALL Consortium Protocol 95-01.

Aihong Li, Jianbiao Zhou, Hongjun Wang, Meredith A. Goldwasser et autres

Abstract During normal T-cell differentiation, T-cell receptor (TCR) genes undergo recombination in an ordered way. TCRd genes rearrange first followed by TCRg genes, which may result in TCRgd+ T lymphocytes. TCRab T lymphocytes develop via a separate differentiation lineage, with TCRb preceding …

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Utilization of Ig heavy chain variable, diversity, and joining gene segments in children with B-lineage acute lymphoblastic leukemia: implications for the mechanisms of VDJ recombination and for pathogenesis

Aihong Li, Montserrat Rué, Jianbiao Zhou, Hongjun Wang et autres

Sequence analysis of the immunoglobulin heavy chain genes (IgH) has demonstrated preferential usage of specific variable (V), diversity (D), and joining (J) genes at different stages of B-cell development and in B-cell malignancies, and this has provided insight into B-cell maturation and …

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Sequence analysis of clonal immunoglobulin and T-cell receptor gene rearrangements in children with acute lymphoblastic leukemia at diagnosis and at relapse: implications for pathogenesis and for the clinical utility of PCR-based methods of minimal residual disease detection

Aihong Li, Jianbiao Zhou, David Zuckerman, Montserrat Rué et autres

Immunoglobulin (Ig) and T-cell receptor (TCR) gene rearrangements provide clonal markers useful for diagnosis and measurement of minimal residual disease (MRD) in acute lymphoblastic leukemia (ALL). We analyzed the sequences of Ig and TCR gene rearrangements obtained at presentation and relapse in …

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Colonization-dependent expression of inducible nitric oxide synthase by the gastrointestinal epithelium

Michael E. Mitchell, Cheryl Lyons, Ellen I. Closs, Mi‐Ae Park et autres

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Automated malaria detection by depolarization of laser light

B. Mendelow, Cheryl Lyons, PHILEMON NHLANGOTHI, MARJORIE TANA et autres

Anecdotal experience with full blood count (FBC) technology incorporating analysis of depolarized laser light (DLL) for the enumeration of eosinophils showed that malaria infection generated unusual distributions in the white cell channels. The objective of this study was to identify and define …

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Characterization of the third member of the MCAT family of cationic amino acid transporters. Identification of a domain that determines the transport properties of the MCAT proteins.

Ellen I. Closs, Cheryl Lyons, Chantal Kelly, James M. Cunningham

We have identified the third member of a family of cationic amino acid transporters in lipopolysaccharide-stimulated murine macrophages. The deduced amino acid sequence of this transporter is the same as MCAT-2 (mouse cationic amino acid transporter-2), the low affinity transporter expressed in …

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Molecular cloning and functional expression of an inducible nitric oxide synthase from a murine macrophage cell line.

Cheryl Lyons, Gregory Orloff, James M. Cunningham

Macrophages activated by exposure to cytokines and/or to endotoxin produce nitric oxide (NO.), a free radical that is a mediator of the host response to infection. Activation induces the expression of nitric oxide synthase, the enzyme that catalyzes formation of NO. from …

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Cloning and expression of the carbon monoxide dehydrogenase genes from strain C2

Gary W. Black, Cheryl Lyons, Elizabeth G. Williams, John Colby et autres

Carbon monoxide dehydrogenase (CODH) from Pseudomonas thermocarboxydovorans strain C2 is composed of three non-identical subunits. A gene library of C2 DNA in lambda vector L47.1 was generated and screened using anti-CODH serum. Western blotting experiments revealed a protein which co-migrated with and …

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Cloning and expression of human aldose reductase.

Chihiro Nishimura, Y. Matsuura, Y Kokai, Tai Akera et autres

The complete amino acid sequence of human retina and muscle aldose reductase was determined by nucleotide analysis of cDNA clones isolated using synthetic oligonucleotide probes based on partial amino acid sequences of purified human psoas muscle aldose reductase. The cDNA sequence differs …

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