Automated malaria detection by depolarization of laser light
Rattachement africain : Afrique du Sud, de. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Anecdotal experience with full blood count (FBC) technology incorporating analysis of depolarized laser light (DLL) for the enumeration of eosinophils showed that malaria infection generated unusual distributions in the white cell channels. The objective of this study was to identify and define criteria for a diagnosis of malaria using this technology. To determine sensitivity, specificity, and positive and negative predictive values, 224 directed samples referred specifically for malaria were used; true positives were defined as those in which malaria was identified by microscopic and/or immunological methods. For the DLL method, positive was defined as one or more large mononuclear cell(s) for which the 90 degrees depolarized signal exceeded the 90 degrees polarized signal. To determine possible utility in a routine haematology laboratory setting, 220 random undirected FBC samples were evaluated for possible malaria infection by the DLL method. Of the 224 directed samples, 95 were malaria positive as determined by microscopic and/or immunological methods, and 129 were negative. For the DLL method, overall sensitivity was 72% (90% in the case of Black Africans), and specificity 96%. Positive and negative predictive values overall were 93% and 82% respectively. In the utility study a single positive result was identified among the 220 samples studied. This was found to be from a patient with malaria. The detection of unexpected malaria by automated screening FBC analysis could substantially lower the mortality and morbidity from unascertained infection, especially in indigenous African peoples.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Automated malaria detection by depolarization of laser light
- Date Crossref
- 01/03/1999
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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South African Institute for Medical Research Department of Haematology Witwatersrand, Afrique du Sud (code pays fourni par la source)Structure de recherche
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University of the Witwatersrand University of the Witwatersrand, Afrique du Sud (code pays fourni par la source)Université ou école supérieure
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Lancet Laboratories Afrique du Sud (code pays fourni par la source)Structure de recherche
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GS1 South Africa Afrique du Sud (code pays fourni par la source)Institution
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Abbott (Germany) pays non établi dans la noticeEntreprise
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Departments of pays non établi dans la noticeInstitution
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Abbott Diagnostics pays non établi dans la noticeInstitution
Department of Haematology — South African Institute for Medical Research (Witwatersrand, Afrique du Sud), University of the Witwatersrand (University of the Witwatersrand, Afrique du Sud) et Lancet Laboratories (Afrique du Sud), avec 4 autres affiliations. Pays d’affiliation : Afrique du Sud.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.