Ilya Grigoriev

Development of the fluorescent probe CenSpark for labeling centrioles and cilia

Cédric Pourroy, Georgios N. Hatzopoulos, Luc Reymond, Friso Douma et autres

Small-molecule probes are transformative for cell biology, offering unprecedented insights into subcellular structures, including in systems without molecular genetic tools. Centrioles are fundamental for generating the axoneme of cilia and flagella, as well as centrosomes, but a generic small-molecule probe allowing selective …

ch, nl (code pays fourni par la source)

Deep Joint Denoising and Detection for Enhanced Intracellular Particle Analysis

Yao Yao, Ihor Smal, Ilya Grigoriev, Anna S. Akhmanova et autres

Reliable analysis of intracellular dynamic processes in time-lapse fluorescence microscopy images requires complete and accurate tracking of all small particles in all time frames of the image sequences. A fundamental first step towards this goal is particle detection. Given the small size …

CSPP1 stabilizes growing microtubule ends and damaged lattices from the luminal side

Cyntha M. van den Berg, Vladimir A. Volkov, Sebastian Schnorrenberg, Ziqiang Huang et autres

Microtubules are dynamic cytoskeletal polymers, and their organization and stability are tightly regulated by numerous cellular factors. While regulatory proteins controlling the formation of interphase microtubule arrays and mitotic spindles have been extensively studied, the biochemical mechanisms responsible for generating stable microtubule …

nl, de, no (code pays fourni par la source)

Opto-katanin, an optogenetic tool for localized, microtubule disassembly

Joyce C. M. Meiring, Ilya Grigoriev, Wilco Nijenhuis, Lukas C. Kapitein et autres

Microtubules are cytoskeletal polymers that separate chromosomes during mitosis and serve as rails for intracellular transport and organelle positioning. Manipulation of microtubules is widely used in cell and developmental biology, but tools for precise subcellular spatiotemporal control of microtubules are currently lacking. …

nl (code pays fourni par la source)

CSPP1 stabilizes growing microtubule ends and damaged lattices from the luminal side

Cyntha M. van den Berg, Vladimir A. Volkov, Sebastian Schnorrenberg, Ziqiang Huang et autres

Abstract Microtubules are dynamic cytoskeletal polymers, and their organization and stability are tightly regulated by numerous cellular factors. While regulatory proteins controlling formation of interphase microtubule arrays and mitotic spindles have been extensively studied, the biochemical mechanisms responsible for generating stable microtubule …

nl, de, no (code pays fourni par la source)

Angiomotin isoform 2 promotes binding of PALS1 to KIF13B at primary cilia and regulates ciliary length and signaling

Stine Kjær Morthorst, Camilla Munch Nielsen, Pietro Farinelli, Zeinab Anvarian et autres

The kinesin-3 motor KIF13B functions in endocytosis, vesicle transport and regulation of ciliary length and signaling. Direct binding of the membrane-associated guanylate kinase (MAGUK) DLG1 to the MAGUK-binding stalk domain of KIF13B relieves motor autoinhibition and promotes microtubule plus-end-directed cargo transport. Here, …

dk, nl, sg (code pays fourni par la source)

Advanced epithelial lung and gut barrier models demonstrate passage of microplastic particles

Joanne M. Donkers, Elena M. Höppener, Ilya Grigoriev, Lena Will et autres

Abstract Micro- and nanoplastics (MNP) can be found virtually everywhere around us in the biosphere and food chain, therefore humans are continuously exposed to MNP, mainly via inhalation and ingestion. Here, we have applied physiologically relevant human-based advanced in vitro models representing …

nl (code pays fourni par la source)

Opto-Katanin: An Optogenetic Tool for Localized Microtubule Disassembly

Joyce C. M. Meiring, Ilya Grigoriev, Wilco Nijenhuis, Lukas C. Kapitein et autres

Abstract Microtubules are major cytoskeletal filaments that drive chromosome separation during cell division, serve as rails for intracellular transport and as a scaffold for organelle positioning. Experimental manipulation of microtubules is widely used in cell and developmental biology, but tools for precise …

nl (code pays fourni par la source)

Angiomotin isoform 2 promotes binding of PALS1 to KIF13B at the base of primary cilia and suppresses ciliary elongation

Stine Kjær Morthorst, Camilla Munch Nielsen, Pietro Farinelli, Zeinab Anvarian et autres

Abstract The kinesin-3 motor KIF13B functions in endocytosis, vesicle transport, and regulation of ciliary length and signaling. Direct binding of the membrane-associated guanylate kinase (MAGUK) DLG1 to KIF13B’s MAGUK-binding stalk (MBS) domain relieves motor autoinhibition and promotes microtubule plus end-directed cargo transport. …

dk, nl (code pays fourni par la source)

Concerted action of kinesins KIF5B and KIF13B promotes efficient secretory vesicle transport to microtubule plus ends

Andrea Serra-Marques, Maud Martin, Eugene A. Katrukha, Ilya Grigoriev et autres

Intracellular transport relies on multiple kinesins, but it is poorly understood which kinesins are present on particular cargos, what their contributions are and whether they act simultaneously on the same cargo. Here, we show that Rab6-positive secretory vesicles are transported from the …

nl, dk (code pays fourni par la source)

Author response: Concerted action of kinesins KIF5B and KIF13B promotes efficient secretory vesicle transport to microtubule plus ends

Andrea Serra-Marques, Maud Martin, Eugene A. Katrukha, Ilya Grigoriev et autres

The function and distribution of kinesin motors on exocytotic vesicles is dissected and visualized through a combination of gene knockout experiments, high-resolution microscopy and advanced data analysis.

nl, dk (code pays fourni par la source)

Deep-learning method for data association in particle tracking

Yao Yao, Ihor Smal, Ilya Grigoriev, Anna S. Akhmanova et autres

MOTIVATION: Biological studies of dynamic processes in living cells often require accurate particle tracking as a first step toward quantitative analysis. Although many particle tracking methods have been developed for this purpose, they are typically based on prior assumptions about the particle …

nl, au (code pays fourni par la source)