Luc Reymond

Author Correction: Development of the fluorescent probe CenSpark for labeling centrioles and cilia

Cédric Pourroy, Georgios N. Hatzopoulos, Luc Reymond, Friso Douma et autres

In the version of the article initially published, the second from right panel in Fig. 6a was labelled “450 min” but should have been labelled “850 min”. The figure has now been corrected in the HTML and PDF versions of the article.

ch, nl (code pays fourni par la source)

HAK-actin, a U-ExM-compatible probe to image the actin cytoskeleton

Olivier Mercey, Luc Reymond, Florent Lemaître, Isabelle Méan et autres

Expansion microscopy (ExM) enables super-resolution imaging by physically enlarging biological samples. While ExM has been successfully applied to study the intracellular microtubule cytoskeleton, reliable probes for visualizing actin fibers remain limited. Here, we present HAK-actin, an engineered actin probe compatible with ultrastructure …

ch, fr (code pays fourni par la source)

Development of the fluorescent probe CenSpark for labeling centrioles and cilia

Cédric Pourroy, Georgios N. Hatzopoulos, Luc Reymond, Friso Douma et autres

Small-molecule probes are transformative for cell biology, offering unprecedented insights into subcellular structures, including in systems without molecular genetic tools. Centrioles are fundamental for generating the axoneme of cilia and flagella, as well as centrosomes, but a generic small-molecule probe allowing selective …

ch, nl (code pays fourni par la source)

A biochemical probe for microtubule lattice integrity uncovers motor-caused lattice damage

Cornelia Egoldt, Luc Reymond, Joshua Tran, Aleksandar Salim et autres

ABSTRACT Microtubules experience mechanical and enzymatic stresses that can compromise lattice integrity, yet where and how lattice damage forms remains poorly understood due to the lack of tools that directly visualize damage as it occurs. Existing approaches infer damage indirectly through repair …

ch (code pays fourni par la source)

CenSpark, a dual-ligand probe for selective labeling of centrioles and cilia_DataSet

Cédric Pourroy, Georgios N. Hatzopoulos, Luc Reymond, Friso Douma et autres

This repository contains the minimum microscopy dataset, related to the main and extended figures, that is necessary to interpret, verify and extend the research on the following article. It also has a file containing the code used to localise centriole signal for …

ch, nl (code pays fourni par la source)

CenSpark, a dual-ligand probe for selective labeling of centrioles and cilia_DataSet

Cédric Pourroy, Georgios N. Hatzopoulos, Luc Reymond, Friso Douma et autres

This repository contains the minimum microscopy dataset, related to the main and extended figures, that is necessary to interpret, verify and extend the research on the following article. It also has a file containing the code used to localise centriole signal for …

ch, nl (code pays fourni par la source)

Machine Learning-Assisted Quantification of Organelle Abundance

Alexander Long, Diogo Candeias, Nicki Coveña, Luc Reymond et autres

Organelle abundance is a key microscopic readout of organelle formation and, in many cases, function. Quantification of organelle abundance using confocal microscopy requires estimating their area based on the fluorescence intensity of compartment-specific markers. This analysis usually depends on a user-defined intensity …

gb, nl, ch (code pays fourni par la source)

A biochemical probe for microtubule lattice integrity uncovers motor-caused lattice damage

Cornelia Egoldt, Luc Reymond, Joshua Tran, Aleksandar Salim et autres

Microtubules experience mechanical and enzymatic stresses that can compromise lattice integrity, yet where and how lattice damage forms remains poorly understood due to the lack of tools that directly visualize damage as it occurs. Existing approaches infer damage indirectly through repair events …

Peroxisome Staining in Mammalian Cells Using Peroxisome-Specific Probes

Charlotte M. Howman, Tamar Cohen, Mor Yarshansky Shlomy, Melanie S. van Aerle et autres

Peroxisomes are ubiquitous organelles with essential roles in human health, and their dysfunction leads to a spectrum of genetic disorders. Visualization of peroxisomes is key to identifying peroxisome dysfunction; however, progress has been limited by the lack of peroxisome-specific probes. By developing …

gb, il, jp, tw, ch (code pays fourni par la source)

Imaging mitochondrial membrane potential via concentration-dependent fluorescence lifetime changes

Dilizhatai Saimi, Luc Reymond, Tursunjan Aziz, Xuan Shen et autres

Abstract Mitochondria are central to cellular metabolism. Various fluorescence tools have been developed for imaging the mitochondrial environment. Yet, new reporters and imaging methods for directly reading the mitochondrial status are needed for high spatial-temporal resolution imaging. Here, we introduce PK Mito …

cn, ch, cz (code pays fourni par la source)

A Silicon Rhodamine‐fused Glibenclamide to Label and Detect Malaria‐infected Red Blood Cells

Claudia Bastl, Cindy Close, Ingo Holtz, Blaise Gatin‐Fraudet et autres

The malaria parasite Plasmodium falciparum affects the lives of millions of people worldwide every year. The detection of replicating parasites within human red blood cells is of paramount importance, requiring appropriate diagnostic tools. Herein, we design and apply a silicon rhodamine-fused glibenclamide …

de, gb, Nigéria, ch, us (code pays fourni par la source)

Progesterone-bound DB3 Fab in complex with computationally designed DBPro1156_2 protein binder

Alan P. Marchand, Stephen Buckley, Arne Schneuing, Martin Pačesa et autres

EMPIAR, the Electron Microscopy Public Image Archive centered at EMBL-EBI, is a public resource for raw electron microscopy images related to EMDB, contains micrographs, particle sets and tilt-series.