Francisco Romero

The Relationship Between TikTok Usage and Executive Function Is Mediated by Problematic Social Media Use

Jordan Golding, Anya Rallison, Aisha Awan, Francisco Romero et autres

Social media is deeply integrated into life, offering new opportunities for learning and communication. However, excessive use has been linked to negative effects on well-being and cognitive functions. A rapidly growing platform, TikTok has been a focal point of controversy due to …

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The Relationship between Tiktok Usage, Social Media Addiction, and Executive Function

Anya Rallison, Aisha Awan, Francisco Romero, Jillia Lacbain et autres

How to study a highly toxic protein to bacteria: A case of voltage sensor domain of mouse sperm-specific sodium/proton exchanger

César Arcos-Hernández, Esteban Suárez-Delgado, León D Islas, Francisco Romero et autres

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Comparative genomic analysis suggests that the sperm-specific sodium/proton exchanger and soluble adenylyl cyclase are key regulators of CatSper among the Metazoa

Francisco Romero, Takuya Nishigaki

CatSper is a sperm-specific calcium ion (Ca 2+ ) channel, which regulates sperm flagellar beating by tuning cytoplasmic Ca 2+ concentrations. Although this Ca 2+ channel is essential for mammalian fertilization, recent bioinformatics analyses have revealed that genes encoding CatSper are heterogeneously …

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High potassium seawater inhibits ascidian sperm chemotaxis, but does not affect the male gamete chemotaxis of a brown alga

Nana Kinoshita‐Terauchi, Kogiku Shiba, Makoto Terauchi, Francisco Romero et autres

Male gamete chemotaxis towards the female gamete is a general strategy to facilitate the sexual reproduction in many marine eukaryotes. Biochemical studies of chemoattractants for male gametes of brown algae have advanced in the 1970s and 1980s, but the molecular mechanism of …

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Robust Evaluation of Intermolecular FRET Using a Large Stokes Shift Fluorophore as a Donor

Carmen Santana‐Calvo, Francisco Romero, Ignacio López‐González, Takuya Nishigaki

Fluorescence (or Förster) resonance energy transfer (FRET) is a straightforward and sensitive technique to evaluate molecular interactions. However, most of the popular FRET pairs suffer cross-excitation of the acceptor, which could lead to false positives. To overcome this problem, we selected a …

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FRET‐based binding assay between a fluorescent cAMP analogue and a cyclic nucleotide‐binding domain tagged with a CFP

Francisco Romero, Carmen Santana‐Calvo, Yoloxochitl Sánchez‐Guevara, Takuya Nishigaki

The cyclic nucleotide-binding domain (CNBD) functions as a regulatory domain of many proteins involved in cyclic nucleotide signalling. We developed a straightforward and reliable binding assay based on intermolecular fluorescence resonance energy transfer (FRET) between an adenosine-3', 5'-cyclic monophosphate analogue labelled with …

Interferón α-2b tópico en el papiloma conjuntival

N. Barriga, Nieves Martín Begué, Francisco Romero

espanolCaso clinico: Paciente varon de 13 anos de edad que consulto por un papiloma conjuntival caruncu- lar que recidivo tras la exeresis quirurgica. Se decidio iniciar tratamiento topico con interferon α-2b con buena tolerancia y regresion progresiva de la lesion. La suspension …

FRET analysis using sperm-activating peptides tagged with fluorescent proteins reveals that ligand-binding sites exist as clusters

César Arcos-Hernández, Francisco Romero, Yoloxochitl Sánchez-Guevara, Carmen Beltrán et autres

Long-range cellular communication between the sperm and egg is critical for external fertilization. Sperm-activating peptides (SAPs) are diffusible components of the outer layer of eggs in echinoderms, and function as chemoattractants for spermatozoa. The decapeptide named speract is the best-characterized sea urchin …

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Intracellular pH in sperm physiology

Takuya Nishigaki, Ana Laura González‐Cota, Francisco Romero, Claudia L. Treviño et autres

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Survey of the presence of bovine herpesvirus type 1 (BoHV-1) in water buffaloes serologically positive to glycoprotein E.

Esterina De Carlo, Gianluca Miletti, Giuseppe Iovane, Giorgio Galiero et autres

Extremely low prevalence of paratuberculosis in Italian buffaloes: biological reality or technical ineffectiveness?

Erminia Sezzi, Aulo Gelli, Giorgio Saralli, T. Zottola et autres