James A. Johnston

Quantitative measurement of the requirement of diverse protein degradation pathways in MHC class I peptide presentation

Jennifer L. Mamrosh, David J. Sherman, Joseph R. Cohen, James A. Johnston et autres

Peptides from degradation of intracellular proteins are continuously displayed by major histocompatibility complex (MHC) class I. To better understand origins of these peptides, we performed a comprehensive census of the class I peptide repertoire in the presence and absence of ubiquitin-proteasome system …

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Supplementary Figure 1 from The Deubiquitinating Enzyme USP17 Is Highly Expressed in Tumor Biopsies, Is Cell Cycle Regulated, and Is Required for G1-S Progression

Cheryl McFarlane, Alyson A. Kelvin, Michelle de la Vega, Ureshnie Govender et autres

Supplementary Figure 1 from The Deubiquitinating Enzyme USP17 Is Highly Expressed in Tumor Biopsies, Is Cell Cycle Regulated, and Is Required for G1-S Progression

Supplementary Figure 1 from The Deubiquitinating Enzyme USP17 Is Highly Expressed in Tumor Biopsies, Is Cell Cycle Regulated, and Is Required for G1-S Progression

Cheryl McFarlane, Alyson A. Kelvin, Michelle de la Vega, Ureshnie Govender et autres

Supplementary Figure 1 from The Deubiquitinating Enzyme USP17 Is Highly Expressed in Tumor Biopsies, Is Cell Cycle Regulated, and Is Required for G1-S Progression

Data from The Deubiquitinating Enzyme USP17 Is Highly Expressed in Tumor Biopsies, Is Cell Cycle Regulated, and Is Required for G1-S Progression

Cheryl McFarlane, Alyson A. Kelvin, Michelle de la Vega, Ureshnie Govender et autres

Abstract Ubiquitination is a reversible posttranslational modification that is essential for cell cycle control, and it is becoming increasingly clear that the removal of ubiquitin from proteins by deubiquitinating enzymes (DUB) is equally important. In this study, we have identified high levels …

Data from The Deubiquitinating Enzyme USP17 Is Highly Expressed in Tumor Biopsies, Is Cell Cycle Regulated, and Is Required for G1-S Progression

Cheryl McFarlane, Alyson A. Kelvin, Michelle de la Vega, Ureshnie Govender et autres

Abstract Ubiquitination is a reversible posttranslational modification that is essential for cell cycle control, and it is becoming increasingly clear that the removal of ubiquitin from proteins by deubiquitinating enzymes (DUB) is equally important. In this study, we have identified high levels …

A Systematic Interrogation of MHC Class I Peptide Presentation Identifies Constitutive and Compensatory Protein Degradation Pathways

Jennifer L. Mamrosh, David J. Sherman, Joseph R. Cohen, James A. Johnston et autres

ABSTRACT The adaptive immune system distinguishes self from non-self by surveying peptides generated from degradation of intracellular proteins that are loaded onto MHC Class I molecules for display on the cell surface. While early studies reported that the bulk of cell surface …

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HLA-A*02:01-directed chimeric antigen receptor/forkhead box P3-engineered CD4+ T cells adopt a regulatory phenotype and suppress established graft-versus-host disease

Aaron D. Martin, Mark Daris, James A. Johnston, Jiajia Cui

BACKGROUND AIMS: To investigate the feasibility of using CD4 + T cells genetically modified to express an allo-HLA directed CAR and FOXP3 to suppress T cell proliferation and cytokine secretion in GvHD. METHODS: Human CD4+ T cells from A*02:01 negative donors were …

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A nanoparticle vaccine that targets neoantigen peptides to lymphoid tissues elicits robust antitumor T cell responses

Carlos Arbelaez, Juan Estrada, Melissa A. Gessner, Charles Glaus et autres

Abstract Cancer vaccines using synthetic long peptides (SLP) targeting tumor antigens have been tested in the clinic but the outcomes have been unimpressive, perhaps because these peptides elicit predominantly CD4+ T cell responses. We hypothesized that enhanced delivery of peptide antigens to, …

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Listeria monocytogenes personalized cancer vaccines drive therapeutic immune responses to cancer derived neoantigens

Brandon Coder, Olga Pryshchep, Dipti Kelkar, Elena V. Filippova et autres

Abstract Background Recent advances in the field of cancer immunotherapy have identified CD8 + T cell responses against tumor-specific mutations as a key driver of tumor regression and overall survival. ADXS-NEO is a personalized Listeria monocytogenes ( Lm )-based immunotherapy designed to …

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Drug Repurposing: The Anthelmintics Niclosamide and Nitazoxanide Are Potent TMEM16A Antagonists That Fully Bronchodilate Airways

Kent Miner, Katja Labitzke, Benxian Liu, Paul Wang et autres

channel, TMEM16A, offers a new mechanism to bronchodilate airways and block the multiple contractiles operating in severe disease. To identify TMEM16A antagonists we screened a library of ∼580,000 compounds. The anthelmintics niclosamide, nitazoxanide, and related compounds were identified as potent TMEM16A antagonists …

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Drug Repurposing: The Anthelmintics Niclosamide and Nitazoxanide are Potent TMEM16A Antagonists that Fully Bronchodilate Airways

Kent Miner, Katja Labitzke, Benxian Liu, Paul Wang et autres

There is an unmet need in severe asthma where approximately 40% of patients exhibit poor β-agonist responsiveness, suffer daily symptoms and show frequent exacerbations. Antagonists of the Ca 2+ -activated-Cl − channel, TMEM16A, offers a new mechanism to bronchodilate airways and block …

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Differing roles for short chain fatty acids and GPR43 agonism in the regulation of intestinal barrier function and immune responses

Warren N. D’Souza, Jason Douangpanya, Sharon Mu, Peter Jaeckel et autres

Inflammatory bowel disease (IBD) is associated with a loss of intestinal barrier function and dysregulated immune responses. It has been shown that short chain fatty acids (SCFAs) are protective in IBD and that GPR43 mediates the protective effects of SCFAs. In this …

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