Ryo Hamamoto

Effect of pH , NaCl concentration, and mAb concentration of feed solution on the filterability of Planova™ 20N and Planova™ BioEX

Chie Hashikawa‐Muto, Yoshiro Yokoyama, Ryo Hamamoto, Kazuya Kobayashi et autres

Virus filtration is one of the most important steps in ensuring viral safety during the purification of monoclonal antibodies (mAbs) and other biotherapeutics derived from mammalian cell cultures. Regarding the various virus retentive filters, including Planova filters, a great deal of data …

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Size‐based analysis of virus removal filter fouling using fractionated protein aggregates

K. Tsukamoto, Ryo Hamamoto, Ryota Oguri, Aki Miura et autres

Fouling by protein aggregates reduces virus removal filter performance. In the present study, we investigated the effects of different-sized protein aggregates on fouling and aggregate retention in order to better understand the fouling mechanisms. Human immunoglobulin G was denatured by heating to …

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Author response for "Size‐based analysis of virus removal filter fouling using fractionated protein aggregates"

K. Tsukamoto, Ryo Hamamoto, Ryota Oguri, Aki Miura et autres

Interactions between protein molecules and the virus removal membrane surface: Effects of immunoglobulin G adsorption and conformational changes on filter performance

Ryo Hamamoto, Hidemi Ito, Makoto Hirohara, Ryongsok Chang et autres

Membrane fouling commonly occurs in all filter types during virus filtration in protein-based biopharmaceutical manufacturing. Mechanisms of decline in virus filter performance due to membrane fouling were investigated using a cellulose-based virus filter as a model membrane. Filter performance was critically dependent …

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