Xinying Zong

Limited sustainability of regulatory T cell repertoire revealed by novel genetic switches 2257672

Lu Bai, Xiaolei Hao, Xinying Zong, Lei Li et autres

Abstract Introduction Regulatory T (Treg) cells carry a diverse T-cell antigen receptor (TCR) repertoire, playing a crucial role in suppressing inflammatory T cells reactive to self-antigens and innocuous foreign antigens. Despite their long lifespan, questions arise about how the diverse Treg repertoire …

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Rag1-based time-stamp tracing of T-cell developmental origins

Menglin Jiang, Yichao Li, Xinying Zong, Xiaolei Hao et autres

The postnatal T‑cell pool comprises cells at diverse developmental stages, complicating efforts to resolve T‑cell differentiation, maturation, and long‑term adaptation. To address this challenge, we developed Rag1‑mediated time-stamp tracing, a genetic strategy that enables time‑resolved tracking of T‑cell developmental origins. We validate …

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T cell receptor signaling induces expression of lysine demethylase KDM6B to maintain Treg homeostasis

Minghong He, Beisi Xu, Pria G. Bose, M. Mccullough et autres

Tregs expressing forkhead box P3 (FOXP3) play crucial roles in maintaining immune tolerance and tissue integrity. EZH2, a histone H3 lysine 27 (H3K27) methyltransferase, is known as a key regulator of Treg identity and suppressive function upon activation. Here, we demonstrate that …

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Temporal genetic switches define the constraints of finite Treg repertoires 9018

Lu Bai, Xinying Zong, Xiao‐Lei Hao, Julia Keith et autres

Abstract Description Foxp3-expressing CD4 regulatory T cells possess a diverse T-cell receptor repertoire and are pivotal in suppressing autoimmune responses, conferring tolerance to innocuous foreign antigens, such as food and commensal microbiota, and maintaining tissue integrity. The Treg repertoire is presumably continuously …

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Deconvolution of developmental age refines thymic regulatory T cell ontogeny 3047

Menglin Jiang, Xinying Zong, Yichao Li, Yong Cheng

Abstract Description Continuous thymic T-cell selection and regulatory T (Treg) cell development challenges studies of their underlying mechanisms in native physiological conditions. Here, we develop a timestamp approach to trace thymic T-cell origins using Rag1CreER knockin mice combined with Rosa reporters. By …

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Dynamic Foxp3–chromatin interaction controls tunable Treg cell function

Ming-Hong He, Xinying Zong, Beisi Xu, Wenjie Qi et autres

Nuclear factor Foxp3 determines regulatory T (Treg) cell fate and function via mechanisms that remain unclear. Here, we investigate the nature of Foxp3-mediated gene regulation in suppressing autoimmunity and antitumor immune response. Contrasting with previous models, we find that Foxp3-chromatin binding is …

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Genetic tracing of thymic T cell differentiation

Menglin Jiang, Julia Keith, Xinying Zong, Xiaolei Hao et autres

Abstract Thymic T cell differentiation is controlled by stereotyped processes that remain obscure. Here we develop a novel mouse genetic tool to trace thymic T cells during T cell antigen receptor (TCR) rearrangement. Briefly, we insert inducible CreER into the Rag1 locus …

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Data from Long Noncoding RNA MALAT1 Promotes Hepatocellular Carcinoma Development by SRSF1 Upregulation and mTOR Activation

Pushkar Malakar, Asaf Shilo, Adi Mogilevsky, Ilan Stein et autres

Abstract Several long noncoding RNAs (lncRNA) are abrogated in cancer but their precise contributions to oncogenesis are still emerging. Here we report that the lncRNA MALAT1 is upregulated in hepatocellular carcinoma and acts as a proto-oncogene through Wnt pathway activation and induction …

Figures S1-S8 from Long Noncoding RNA MALAT1 Promotes Hepatocellular Carcinoma Development by SRSF1 Upregulation and mTOR Activation

Pushkar Malakar, Asaf Shilo, Adi Mogilevsky, Ilan Stein et autres

Figure S1: Knockdown of MALAT1 inhibits proliferation of liver progenitor and HCC cells. Figure S2: Effect of MALAT1 expression on splicing of endogenous SRSF1 targets. Figure S3. Differential gene expression based on RNA-seq data. Figure S4. Enriched pathways and networks activated by …

Supplementary Materials and Methods and Supplementary Figure Legends from Long Noncoding RNA MALAT1 Promotes Hepatocellular Carcinoma Development by SRSF1 Upregulation and mTOR Activation

Pushkar Malakar, Asaf Shilo, Adi Mogilevsky, Ilan Stein et autres

Supplementary information text: Methods and figure legends

Table S1 from Long Noncoding RNA MALAT1 Promotes Hepatocellular Carcinoma Development by SRSF1 Upregulation and mTOR Activation

Pushkar Malakar, Asaf Shilo, Adi Mogilevsky, Ilan Stein et autres

Table S1: List of primers oligos and siRNAs

Table S2 from Long Noncoding RNA MALAT1 Promotes Hepatocellular Carcinoma Development by SRSF1 Upregulation and mTOR Activation

Pushkar Malakar, Asaf Shilo, Adi Mogilevsky, Ilan Stein et autres

Table S2. Full list of Up- and Down regulated genes in the RNA seq analysis