Tony Brooks

Characterizing the Human Fetal Perimeiotic 45,X Ovary at Single-Cell Resolution

Sinead Mary McGlacken-Byrne, Ignacio del Valle, Theodoros A. Xenakis, Jenifer P. Suntharalingham et autres

Abstract Context Turner syndrome (TS) is the most common genetic cause of premature (primary) ovarian insufficiency (POI). Human fetal 45,X ovaries demonstrate marked apoptosis by 15 to 20 weeks post conception (wpc), likely partly driven by X-chromosome haploinsufficiency. However, the genomic drivers …

in, gb (code pays fourni par la source)

Mapping the anatomical and transcriptional landscape of early human fetal ovary development

Sinead Mary McGlacken-Byrne, Ignacio del Valle, Theodoros A. Xenakis, Ian C. Simcock et autres

The complex genetic mechanisms underlying human ovary development can give rise to clinical phenotypes if disrupted, such as Primary (or Premature) Ovarian Insufficiency and Differences of Sex Development. We combine single-nuclei RNA sequencing, bulk RNA sequencing, and micro-focus computed tomography to elucidate …

in, gb (code pays fourni par la source)

Transcriptomic sex differences in early human fetal brain development

Federica Buonocore, Jenifer P. Suntharalingham, Olumide Kayode Ogunbiyi, Aragorn Jones et autres

The influence of sex chromosomes and sex hormones on early human brain development is poorly understood. We therefore undertook transcriptomic analysis of 46,XY and 46,XX human brain cortex samples (n = 64) at four different time points between 7.5 and 17 weeks …

in, gb (code pays fourni par la source)

Nanopore-based hybridization capture approaches for targeted viral metagenomics and whole-genome sequencing

Oscar Enrique Torres Montaguth, Sarah Buddle, Leysa Forrest, Tony Brooks et autres

Abstract Hybridization capture approaches can significantly increase the sensitivity of metagenomics. However, their implementation with Oxford Nanopore Technologies (ONT) sequencing has been limited due to the lack of dedicated workflows and the need for an extra ligation-based library preparation step. We developed …

ca, gb (code pays fourni par la source)

Mapping the anatomical and transcriptional landscape of early human fetal ovary development

Sinead Mary McGlacken-Byrne, Valle Ignacio Del, Theodoros A. Xenakis, Ian C. Simcock et autres

Evaluating metagenomics and targeted approaches for diagnosis and surveillance of viruses

Sarah Buddle, Leysa Forrest, Naomi Akinsuyi, Luz Marina Martin Bernal et autres

BACKGROUND: Metagenomics is a powerful approach for the detection of unknown and novel pathogens. Workflows based on Illumina short-read sequencing are becoming established in diagnostic laboratories. However, high sequencing depth requirements, long turnaround times, and limited sensitivity hinder broader adoption. We investigated …

gb, au, ca (code pays fourni par la source)

Evaluating metagenomics and targeted approaches for diagnosis and surveillance of viruses

Sarah Buddle, Leysa Forrest, Naomi Akinsuyi, Luz Marina Martin Bernal et autres

Abstract Background Metagenomics is a powerful approach for the detection of unknown and novel pathogens. Workflows based on Illumina short-read sequencing are becoming established in diagnostic laboratories. However, barriers to broader take-up include the need for high sequencing depths, long turnaround times, …

gb, ca, au (code pays fourni par la source)

Sex differences in early human fetal brain development

Federica Buonocore, Jenifer P. Suntharalingham, Olumide Kayode Ogunbiyi, Aragorn Jones et autres

Abstract The influence of sex chromosomes and sex hormones on early human brain development is poorly understood. We therefore undertook transcriptomic analysis of 46,XY and 46,XX human brain cortex samples (n=64) at four different time points between 7.5 and 17 weeks post …

gb (code pays fourni par la source)

LB1723 Knocking down KLK5 by base editing restored desmoglein I expression in Netherton syndrome keratinocytes

Vignesh Jayarajan, Giuseppe Naso, Tony Brooks, Waseem Qasim et autres

gb (code pays fourni par la source)

Astrovirus VA1/HMO-C: An Increasingly Recognized Neurotropic Pathogen in Immunocompromised Patients

Julianne Rose Brown, Sofia Morfopoulou, Jonathan Hubb, Warren Emmett et autres

BACKGROUND: An 18-month-old boy developed encephalopathy, for which extensive investigation failed to identify an etiology, 6 weeks after stem cell transplant. To exclude a potential infectious cause, we performed high-throughput RNA sequencing on brain biopsy. METHODS: RNA-Seq was performed on an Illumina …

gb (code pays fourni par la source)

Measuring Parent Involvement in Relation to Student Achievement

Tony Brooks

The purpose of this study was to examine the role of parent involvement in relation to the influence on student achievement within a school community. In addition, this study worked towards gaining an understanding of the parent's role in the school community. …

LIMaS: the JAVA-based application and database for microarray experiment tracking

Sarah C. Webb, Anthony Attwood, Tony Brooks, Tom C. Freeman et autres

us, gb (code pays fourni par la source)