Kazuki Ikeda

Proteolytic EphA2 fragments cooperatively promote hepatocellular carcinoma progression

Kazuki Ikeda, Nobuhiko Asakura, Soyogi Sengoku, Eiki Tsukamoto et autres

Abstract EphA2 is a receptor tyrosine kinase that suppresses tumor growth when bound by its ligand ephrin-A1 (EA1), but promotes tumor progression in the absence of ligand. In hepatocellular carcinoma (HCC) cells, EphA2 is proteolytically cleaved by membrane-type 1 matrix metalloproteinase (MT1-MMP), …

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Amino acid–dependent TSC2 dephosphorylation by lysosome–PP2A regulates mTORC1 signaling transduction

Takanori Nakamura, Shigeyuki Nada, Masaki Matsumoto, Nuha Loling Othman et autres

The mammalian target of rapamycin complex 1 (mTORC1) signaling pathway, composed of amino acid (AA)-sensing (Ragulator/LAMTOR-Rag) and growth factor (GF)-sensing (AKT-TSC1/2-Rheb) axes, pivotally regulates intracellular anabolism and catabolism. mTORC1 deregulation is associated with various metabolic diseases, including cancer and diabetes. As a …

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Comparison of Amine-Modified Polymeric Stationary Phases for Polar Metabolomic Analysis Based on Unified-Hydrophilic Interaction/Anion Exchange Liquid Chromatography/High-Resolution Mass Spectrometry (Unified-HILIC/AEX/HRMS)

Kazuki Ikeda, Masatomo Takahashi, Takeshi Bamba, Yoshihiro Izumi

In metabolomic analysis, one of the most commonly used techniques to support the detection sensitivity and quantitation of mass spectrometry is combining it with liquid chromatography. Recently, we developed a method that enables comprehensive single-run measurement of hydrophilic metabolites using unified-hydrophilic interaction/anion …

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Predicting Retention Time in Unified-Hydrophilic-Interaction/Anion-Exchange Liquid Chromatography High-Resolution Tandem Mass Spectrometry (Unified-HILIC/AEX/HRMS/MS) for Comprehensive Structural Annotation of Polar Metabolome

Taihei Torigoe, Masatomo Takahashi, Omid Reza Heravizadeh, Kazuki Ikeda et autres

The accuracy of the structural annotation of unidentified peaks obtained in metabolomic analysis using liquid chromatography/tandem mass spectrometry (LC/MS/MS) can be enhanced using retention time (RT) information as well as precursor and product ions. Unified-hydrophilic-interaction/anion-exchange liquid chromatography high-resolution tandem mass spectrometry (unified-HILIC/AEX/HRMS/MS) …

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Comprehensive Annotation for Unidentified Hydrophilic Metabolites Based on LC/HRMS/MS and In Silico Epimetabolite Database (IEMDB)

Taihei Torigoe, Masatomo Takahashi, Motonao Nakao, Yuki Soma et autres

Epimetabolite is defined as analogues of known metabolites with different substructures. The rapid development of high-resolution mass spectrometry (HRMS) and some data mining tools has contributed to detecting and identifying a few epimetabolites that could play an important role as biological functions. …

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Proteolytic cleavage of membrane proteins by membrane type‐1 MMP regulates cancer malignant progression

Kazuki Ikeda, Ryo Kaneko, Eiki Tsukamoto, Nobuaki Funahashi et autres

Strategies to develop cancer therapies using inhibitors that target matrix metalloproteinases (MMPs), particularly membrane type-1 MMP (MT1-MMP), have failed. This is predominantly attributed to the specificity of MMP inhibitors and numerous functions of MMPs; therefore, targeting substrates with such broad specificity can …

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Expression of Cancer Stem Cell Markers EpCAM and CD90 Is Correlated with Anti- and Pro-Oncogenic EphA2 Signaling in Hepatocellular Carcinoma

Nobuhiko Asakura, Naotoshi Nakamura, Atsushi Muroi, Yosui Nojima et autres

Hepatocellular carcinoma (HCC) is the third leading cause of cancer death worldwide. Additionally, the efficacy of targeted molecular therapies with multiple tyrosine kinase inhibitors is limited. In this study, we focused on the cellular signaling pathways common to diverse HCC cells and …

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Generation of human induced pluripotent stem cell-derived liver buds with chemically defined and animal origin-free media

Keisuke Sekine, Shimpei Ogawa, Syusaku Tsuzuki, Tatsuya Kobayashi et autres

Advances in organoid technology have broadened the number of target diseases and conditions in which human induced pluripotent stem cell (iPSC)-based regenerative medicine can be applied; however, mass production of organoids and the development of chemically defined, animal origin-free (CD-AOF) media and …

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Robust detection of undifferentiated iPSC among differentiated cells

Keisuke Sekine, Syusaku Tsuzuki, Ryota Yasui, Tatsuya Kobayashi et autres

Recent progress in human induced pluripotent stem cells (iPSC) technologies suggest that iPSC application in regenerative medicine is a closer reality. Numerous challenges prevent iPSC application in the development of numerous tissues and for the treatment of various diseases. A key concern …

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Recycling of textile sludge for removing textile dye of reactive red 231 on aqueous solution

Ari Rahman, Naoyuki Kishimoto, Takeo Urabe, Kazuki Ikeda

Abstract Carbonization treatment successfully converted textile sludge into potential adsorbent. The carbonization was performed at the range of 400 to 800 °C without oxygen involved. In order to investigate the adsorption performance of carbonized sludge then a series of adsorption tests was …

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Oral administration of l-ornithine increases the content of both collagen constituting amino acids and polyamines in mouse skin

Daiki Harada, Satsuki NAGAMACHI, Kenta Aso, Kazuki Ikeda et autres

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Methylene blue removal by carbonized textile sludge-based adsorbent

Ari Rahman, Naoyuki Kishimoto, Takeo Urabe, Kazuki Ikeda

Abstract Colored effluent and a large amount of sludge are major pollutant sources derived from textile industry activity. In this research, the idea for converting textile sludge into a potential adsorbent was conducted through a carbonization process in order to solve the …

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