Shuping Xu

Catalytic Hairpin Assembly-Aided CRISPR/Cas-SERS for Nucleic Acid Detection Enhanced by Multiple Catalytic Amplification

Lin Liu, Chengxin Bao, Huimin Wang, Feiwu Li et autres

Abstract This study established a surface-enhanced Raman scattering (SERS) detection method based on the catalytic hairpin assembly (CHA) reaction combined with clustered regularly interspaced short palindromic repeats and its associated proteins (CRISPR/Cas), integrated with the guanine-quadruplexes/Hemin (G4/Hemin) catalytic amplification effect, which is …

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SERS Studies on the Dynamic Response of Membrane Interface Lipids to Proteins: Concentration Effect and Redox State Conversion

Ji Sha, Xin Wang, Y Zhou, Yuehua Chen et autres

The dynamic behavior of lipids at membrane interfaces is essential for maintaining the structural stability and functional specificity of biomembranes, with its regulation closely linked to protein interactions and the redox microenvironment. Understanding the interaction between proteins and phospholipid membranes, as well …

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Label-Free SERS Analysis of Glioma Cells to Detect Drug Resistance Using Metabolic Phenylalanine Level

Guohui Yang, Xin Wang, Jingbin Jin, Xiaozhang Qu et autres

Abstract Glioma is one of the most common malignant brain tumors, and its mainstream clinical treatment regimens mainly include postoperative combined with Temozolomide chemotherapy. Unfortunately, glioma cells tend to mutate when subjected to prolonged Temozolomide treatment, leading to drug resistance and significantly …

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CRISPR/Cas12a and CHA-based SERS platform for ultrasensitive nucleic acid detection

Huimin Wang, Chengxin Bao, Lin Liu, Feiwu Li et autres

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CRISPR/dCas9-Assisted On-Bead Multiplex Detection (BeadPlex2) for Genetically Modified Crops

Huimin Wang, Feiwu Li, Yuxuan He, Xiangguo Liu et autres

This study leverages the precise recognition ability of CRISPR/dCas9 and the Raman coding feature of the gap-enhanced Raman tag-encoded magnetic beads (MagGERTs) to create a unique on-bead nucleic acid detection platform (BeadPlex2) for accurate and multiplex nucleic acid detection, which was proven …

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Lipid Metabolism-Related Rapid Pathological Diagnosis and Staging of Breast Tissue Sections Using Two-Photon Fluorescence Imaging Assisted with a Carbonized Polymer Dot Probe

Zhiyi Yu, Jiaqi Wang, Ying Liu, Weiqing Xu et autres

Abstract Rapid and accurate intraoperative pathologic diagnosis is essential for intraoperative decision-making, improving patient prognosis, and avoiding reoperation. This study developed a unique rapid pathological diagnosis and staging technique for intraoperative tissue sections based on lipidomic relevance, using two-photon fluorescence (TPF) imaging …

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Machine Learning‐Enhanced Analysis of Exosomal Surface Sialic Acid Using Surface‐Enhanced Raman Spectroscopy for Ovarian Cancer Diagnosis and Therapeutic Monitoring

Lili Cong, Jiaqi Wang, Sijun Huang, Xiaxia Man et autres

Currently, the absence of ovarian cancer (OC)-specific biomarkers impedes the development of precise noninvasive diagnostic and monitoring strategies. Exosomal surface sialic acid (SA), a key mediator of intercellular communication and disease progression, emerges as a promising biomarker, though its role in OC …

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A low-cost CHA-integrated CRISPR/Cas12a-based test strip platform for on-site gene detection

Huimin Wang, Lin Liu, Chengxin Bao, Feiwu Li et autres

-cleavage) can detect concentrations as low as 10 pM in approximately 50 min, without the need for pre-amplification of the target nucleic acid. This detection system ensures high sensitivity and specificity while effectively avoiding non-specific activation. In practical applications with transgenic maize …

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Correction to “Surface Plasmon-Coupled Directional Emission Enhanced Raman Scattering Based on Waveguide Coupling Surface Plasmon Resonance Configuration”

Yu Liu, Jingqiu Liang, Shuping Xu, Yijia Geng

Development of a Diagnostic Model for Colorectal Cancer Utilizing Surface-Enhanced Raman Spectroscopy Coupled with Machine Learning

Tingdong Jiang, Hongwei Zhang, Yonghui Sun, Daguang Wang et autres

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Research on a Method of Improving Cartographer Algorithm

Yinglong Li, Shuping Xu

In complex scenes, dynamic objects can cause significant deviations in the accurate estimation of the robot's pose. This paper proposes a visual SLAM algorithm based on an improved YOLOv5s. The algorithm utilizes the YOLOv5s object detection method to identify dynamic objects in …

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Multiplex detection of genetically modified maize events via CRISPR/dCas9 integrated dual-coded bead assay

Huimin Wang, Feiwu Li, Yuxuan He, Xiangguo Liu et autres

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