Accès ouvert
2025
preprint
OpenAlex
Matty Gaines, Daniel Parrell, Kaylee Jo Rajek, Steve Garvis et autres
Abstract Prokaryotic chromosomal DNA is compacted and protected by nucleoid-associated proteins (NAPs). Among these, the DNA-binding proteins from starved cells (Dps) combine ferroxidase activity with nonspecific DNA binding to form dodecameric assemblies that condense and safeguard the genome under stress. Here, we …
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Accès ouvert
2025
article
OpenAlex
Jie E Yang, Bryan S. Sibert, Daniel Parrell, Eric Montemayor et autres
Cryo-electron tomography (cryo-ET) has emerged as a leading technique for visualizing large, transient, dynamic, and heterogenous macromolecules in their native or near-native states. From three-dimensional reconstructions (e.g., tomograms) of these complex systems, abundant molecules can be further analyzed for high-resolution structural information …
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Accès ouvert
2024
article
OpenAlex
Daniel Parrell, Joseph Olson, Rachelle A. S. Lemke, Timothy J. Donohue et autres
to investigate the accumulation of carbon and inorganic phosphate in the storage organelles, polyhydroxybutyrate (PHB) and polyphosphate (PP), respectively. Using cryo-electron tomography (cryo-ET), these organelles were observed to increase in size and abundance when growth was arrested by chloramphenicol treatment. The accumulation …
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Accès ouvert
2024
dataset
OpenAlex
Juan C Sanchez, Montemayor EJ, Nicoleta Ploscariu, Daniel Parrell et autres
EMPIAR, the Electron Microscopy Public Image Archive centered at EMBL-EBI, is a public resource for raw electron microscopy images related to EMDB, contains micrographs, particle sets and tilt-series.
Accès ouvert
2024
dataset
OpenAlex
Juan C Sanchez, Montemayor EJ, Nicoleta Ploscariu, Daniel Parrell et autres
EMPIAR, the Electron Microscopy Public Image Archive centered at EMBL-EBI, is a public resource for raw electron microscopy images related to EMDB, contains micrographs, particle sets and tilt-series.
Accès ouvert
2023
article
OpenAlex
Jie E Yang, Matt Larson, Bryan S. Sibert, Joseph Y. Kim et autres
Imaging large fields of view while preserving high-resolution structural information remains a challenge in low-dose cryo-electron tomography. Here we present robust tools for montage parallel array cryo-tomography (MPACT) tailored for vitrified specimens. The combination of correlative cryo-fluorescence microscopy, focused-ion-beam milling, substrate micropatterning, …
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Accès ouvert
2023
article
OpenAlex
Daniel Parrell, Rachelle A. S. Lemke, Joseph Olson, Timothy J. Donohue et autres
Bacteria employ many mechanisms for resisting and responding to environmental stressors. In this work we explore the production of polyhydroxybutyrate (PHB) and polyphosphate (PP) granules in response to antibiotic stress from chloramphenicol. Storage granules in bacteria and other organisms are produced to …
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Accès ouvert
2023
conference-abstract
OpenAlex
Elizabeth Wright, Jae‐Won Yang, Bryan S. Sibert, Matt Larson et autres
Cells are ubiquitous to all living organisms. Cells are either ‘simple’ in their singularity or are the ‘building blocks’ of complex multi-cellular communities. Many advances in microscopy, especially cryo-microscopy techniques, have allowed researchers to begin to investigate the finite detail of cells, …
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Accès ouvert
2023
article
OpenAlex
Juan C Sanchez, Eric Montemayor, Nicoleta Ploscariu, Daniel Parrell et autres
The bacterial flagellum is a complex, multi-protein structure consisting of a basal body, motor, hook, and filament [1]. The filament acts as an Archimedes’ screw propelling the cell body forward and is comprised of thousands of monomers called flagellins [2]. Flagellum biogenesis …
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Accès ouvert
2023
preprint
OpenAlex
Juan C Sanchez, Eric Montemayor, Nicoleta Ploscariu, Daniel Parrell et autres
Abstract Flagella are dynamic, ion-powered machines with assembly pathways that are optimized for efficient flagella production. In bacteria, dozens of genes are coordinated at specific times in the cell lifecycle to generate each component of the flagellum. This is the case for …
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Accès ouvert
2023
article
OpenAlex
Bryan D. Lakey, François Alberge, Daniel Parrell, Elizabeth Wright et autres
ABSTRACT Cell elongation and division are essential aspects of the bacterial life cycle that must be coordinated for viability and replication. The impact of misregulation of these processes is not well understood as these systems are often not amenable to traditional genetic …
us
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2022
article
OpenAlex
Daniel Parrell, Rachelle A. S. Lemke, Joseph Olson, Timothy J. Donohue et autres
Daniel Parrell, Rachelle AS Lemke, Joseph Olson, Timothy J Donohue, Elizabeth R Wright; Effects of Chloramphenicol Treatment on Cellular Storage Granules a
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