Accès ouvert
2021
article
OpenAlex
Elizabeth C. Stahl, Allan Gopez, Connor A. Tsuchida, Vinson B. Fan et autres
Clinical and surveillance testing for the SARS-CoV-2 virus relies overwhelmingly on RT-qPCR-based diagnostics, yet several popular assays require 2-3 separate reactions or rely on detection of a single viral target, which adds significant time, cost, and risk of false-negative results. Furthermore, multiplexed …
us
(code pays fourni par la source)
Accès ouvert
2021
article
OpenAlex
Jennifer Hamilton, Elizabeth C. Stahl, Connor A. Tsuchida, Enrique Lin-Shiao et autres
Saliva is an attractive specimen type for asymptomatic surveillance of COVID-19 in large populations due to its ease of collection and its demonstrated utility for detecting RNA from SARS-CoV-2. Multiple saliva-based viral detection protocols use a direct-to-RT-qPCR approach that eliminates nucleic acid …
us
(code pays fourni par la source)
Accès ouvert
2021
article
OpenAlex
Alexander J. Ehrenberg, Erica A. Moehle, Cara E. Brook, Andrew H. Doudna Cate et autres
Regular surveillance testing of asymptomatic individuals for SARS-CoV-2 has been center to SARS-CoV-2 outbreak prevention on college and university campuses. Here we describe the voluntary saliva testing program instituted at the University of California, Berkeley during an early period of the SARS-CoV-2 …
us
(code pays fourni par la source)
Accès ouvert
2021
preprint
OpenAlex
Alexander J. Ehrenberg, Erica A. Moehle, Cara E. Brook, Andrew H. Doudna Cate et autres
Summary Regular surveillance testing of asymptomatic individuals for SARS-CoV-2 has played a vital role in SARS-CoV-2 outbreak prevention on college and university campuses. Here we describe the voluntary saliva testing program instituted at the University of California, Berkeley during an early period …
us
(code pays fourni par la source)
Accès ouvert
2021
preprint
OpenAlex
Jennifer Hamilton, Elizabeth C. Stahl, Connor A. Tsuchida, Enrique Lin-Shiao et autres
Saliva is an attractive specimen type for asymptomatic surveillance of COVID-19 in large populations due to its ease of collection and its demonstrated utility for detecting RNA from SARS-CoV-2. Multiple saliva-based viral detection protocols use a direct-to-RT-qPCR approach that eliminates nucleic acid …
us
(code pays fourni par la source)
Accès ouvert
2021
article
OpenAlex
Elizabeth C. Stahl, Allan Gopez, Connor A. Tsuchida, Vinson B. Fan et autres
Clinical and surveillance testing for the SARS-CoV-2 virus relies overwhelmingly on RT-qPCR-based diagnostics, yet several popular assays require 2–3 separate reactions or rely on detection of a single viral target, which adds significant time, cost, and risk of false-negative results. Furthermore, multiplexed …
Accès ouvert
2020
preprint
OpenAlex
Elizabeth C. Stahl, Connor A. Tsuchida, Jennifer Hamilton, Enrique Lin-Shiao et autres
Commonly used RT-qPCR-based SARS-CoV-2 diagnostics require 2-3 separate reactions or rely on detection of a single viral target, adding time and cost or risk of false-negative results. Currently, no test combines detection of widely used SARS-CoV-2 E- and N-gene targets and a …
us
(code pays fourni par la source)
Accès ouvert
2020
article
OpenAlex
Alexandra M. Amen, Kerrie Barry, John M. Boyle, Cara E. Brook et autres
us
(code pays fourni par la source)
Accès ouvert
2018
preprint
OpenAlex
Ariana Hirsh
Protocol for the expression and purification of Cas9 protein
Accès ouvert
2018
article
OpenAlex
Romain Rouet, Benjamin A. Thuma, Marc D. Roy, Nathanael G. Lintner et autres
CRISPR-Cas RNA-guided endonucleases hold great promise for disrupting or correcting genomic sequences through site-specific DNA cleavage and repair. However, the lack of methods for cell- and tissue-selective delivery currently limits both research and clinical uses of these enzymes. We report the design …
us
(code pays fourni par la source)
2018
article
OpenAlex
Marc D. Roy, Romain Rouet, Benjamin A. Thuma, Nathanael G. Lintner et autres
CRISPR‐Cas RNA‐guided endonucleases hold great promise for disrupting or correcting genomic sequences through site‐specific DNA cleavage and repair. However, the lack of methods for cell‐ and tissue‐selective delivery currently limits both research and clinical uses of these enzymes. Here we report the …
us
(code pays fourni par la source)
2017
conference-abstract
OpenAlex
Lu Liu, Raffael Nachbagauer, Lingyan Zhu, Yang Bin Huang et autres
Abstract Background The outbreak of novel avian H7N9 influenza virus infections in China in 2013 has demonstrated the continuing threat posed by zoonotic pathogens. To decipher the responses during natural infection will enlighten the vaccine development. Methods We assessed the induction of …
cn, us
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