Daisuke Kuroda

Reversible homodimerization of a small leucine-rich proteoglycan PRELP modulates the interactions with TGFβ1 and IGFI-R

Hirofumi Kosuge, Makoto Nakakido, Daisuke Kuroda, Kouhei Tsumoto

Small leucine-rich proteoglycans (SLRPs) are responsible for various biological functions via their multiple interactions with various proteins. Although some SLRPs form homodimers, it remains unclear how homodimerization affects the molecular functions of SLRPs. Herein, we report the molecular basis and functional significance …

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Rapidly Progressive Multiple Hepatic Hemangiomas Mimicking Angiosarcoma: A Case Report With Surgical and Interventional Management

Asuka Ono, TOSHIRO MASUDA, Hirohisa Okabe, MASAHIRO TOMITA et autres

BACKGROUND/AIM: Hepatic hemangioma is the most common benign liver tumor and typically shows slow or no growth. Rapidly enlarging hemangiomas are rare and distinguishing them from hepatic angiosarcoma can be challenging. CASE REPORT: A 39-year-old woman presented with multiple hepatic hemangiomas that …

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Medial-to-lateral approach for laparoscopic right hepatectomy of the remnant liver: a safe technique for patients with a history of posterior segment resection (with video)

Hidetoshi Gon, Tadahiro Goto, Michihiko Yoshida, Yuki Azumi et autres

Laparoscopic repeat hepatectomy (LRH) remains challenging when initial resection sites overlap with target areas. We report a safe surgical approach for LRH of the remnant right liver after prior posterior segment resection. An 83-year-old female with recurrent intrahepatic cholangiocarcinoma in segment 5, …

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Intra-patient neuraminidase mutations in avian H5N1 influenza virus reduce sialidase activity to complement weaker hemagglutinin binding and facilitate human infection

Y. Watanabe, Madiha S. Ibrahim, Yasuha Arai, Daisuke Kuroda et autres

Clade 2.2 H5N1 influenza viruses have caused an unusually high number of human infections, providing a unique opportunity to investigate early molecular steps associated with host adaptation. Although most work has focused on hemagglutinin (HA), the contribution of neuraminidase (NA) to these …

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A case of multiple gastric adenocarcinoma of fundic gland type accidentally found by additional gastrectomy following endoscopic submucosal dissection

Hiroshi Takeno, Hirohisa Okabe, Hidetoshi Nitta, Masahiro Tomita et autres

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Optimizing the breadth of SARS-CoV-2-neutralizing antibodies in vivo and in silico

Daisuke Kuroda, Saya Moriyama, Hiroaki Sasaki, Yoshimasa Takahashi

Since the emergence of SARS-CoV-2, the ongoing arms race between mutating viruses and human antibodies has revealed several novel strategies by which antibodies adapt to viral escape. While SARS-CoV-2 viruses exhibit high variability in epitopes targeted by neutralizing antibodies, certain epitopes remain …

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Developing FGF2 Mutants with Selectively Reduced Heparan Sulfate Affinity to Explore their Impact on FGFR1 Signaling

Yuga Okada, Akihiro Eguchi, Daisuke Kuroda, Kouhei Tsumoto et autres

Fibroblast growth factor 2 (FGF2)regulates signal transduction by forming complexes with its receptors, FGF receptors (FGFRs), and heparan sulfate (HS), playing a crucial role in biological systems. Although HS has been suggested to modulate FGF/FGFR signaling as a coreceptor, multiple hypotheses exist …

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Improving the solubility of single domain antibodies using VH ‐like hallmark residues

Makoto Nakakido, Takanori Yokoo, Jorge Fernandez‐Perez, Kevin C. Entzminger et autres

Single domain antibodies (sdAbs) can be generated from variable regions of heavy-chain antibodies, which lack light chain and CH1 region. They have attracted attention due to their small size and molecular characteristics. Hydrophilic hallmark amino acids at framework region 2 (FR2) are …

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Development of a small compound that regulates the function of a maltodextrin-binding protein of Streptococcus pyogenes by multifaceted screenings

Tsukushi Yamawaki, Makoto Nakakido, Satoru Nagatoishi, José M. M. Caaveiro et autres

Group A Streptococcus (GAS) are gram-positive bacteria that cause various symptoms. The treatment of GAS infections currently relies on antibiotics, but new treatment options are needed due to the spread of antibiotic resistance. To develop novel treatment methods that circumvent the generation …

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Supercharging design of an anti-lysozyme Fab antibody to regulate ligand-dependent reversible aggregation

Keisuke Kasahara, Makoto Nakakido, Daisuke Kuroda, Satoru Nagatoishi et autres

Abstract Protein aggregation and liquid‒liquid phase separation (LLPS), as key physicochemical processes, orchestrate protein behavior and function, and engineering a protein surface charge offers a robust approach to modulate protein‒protein interactions and, consequently, aggregation and phase separation. Among protein surface engineering methods, …

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Affinity‐stability trade‐off mechanism of residue 35 in framework region 2 of VHH antibodies with β‐hairpin CDR3

Koichi Yamamoto, Satoru Nagatoishi, Ryo Matsunaga, Makoto Nakakido et autres

Abstract Single‐domain VHH antibodies are promising therapeutic and diagnostic tools. The third complementarity‐determining region (CDR3) is usually the most critical region for antigen recognition by VHH antibodies. When CDR3 adopts a short and extended β‐hairpin conformation, framework region 2 (FR2) often interacts …

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Treatment strategy and clinical outcomes of thoracoscopic endoscopic cooperative surgery for submucosal tumors in the esophagus

Yasunori Otowa, Fumiaki Kawara, Gosuke Takiguchi, Kodai Yamanaka et autres

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