Igor Chizhov

Effect of Deposition Modes on Micromechanical Properties of Nanostructured TiAlSiN and TiAlSiCN Coatings

С. В. Константинов, Ф. Ф. Комаров, Igor Chizhov, В. А. Зайков

Abstract Nanostructured nitride TiAlSiN and carbonitride TiAlSiCN coatings were formed by reactive magnetron sputtering on various types of substrates. Elemental composition was studied by energy dispersive X-ray spectroscopy (EDX) as well as structure by X-ray diffraction and micromechanical properties by nanoindentation. It …

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Author response for "Engineering of soluble bacteriorhodopsin"

Andrey Nikolaev, Yaroslav Orlov, Fedor Tsybrov, Elizaveta Kuznetsova et autres

Proteorhodopsin insights into the molecular mechanism of vectorial proton transport

Sergey Bukhdruker, Ivan Gushchin, Vitaly Shevchenko, Kirill Kovalev et autres

Bacterial proton pumps, proteorhodopsins (PRs), are a major group of light-driven membrane proteins found in marine bacteria. They are functionally and structurally distinct from archaeal and eukaryotic proton pumps. To elucidate the proton transfer mechanism by PRs and understand the differences to …

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Engineering of soluble bacteriorhodopsin

Andrey Nikolaev, Yaroslav Orlov, Fedor Tsybrov, Elizaveta Kuznetsova et autres

Studies and applications of membrane proteins remain challenging due to the requirement of maintaining them in a lipid membrane or a membrane mimic. Modern machine learning-based protein engineering methods offer a possibility of generating soluble analogs of membrane proteins that retain the …

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Engineering of soluble bacteriorhodopsin

Andrey Nikolaev, Yaroslav Orlov, Fedor Tsybrov, Elizaveta Kuznetsova et autres

Abstract Bacteriorhodopsin is a seven-helical light-driven proton pump and a model membrane protein. Here, we report engineering of soluble analogues of bacteriorhodopsin, NeuroBRs, which bind retinal and photocycle under illumination. We also report the crystallographic structure of NeuroBR_A, determined at anisotropic resolution …

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Production of eukaryotic heliorhodopsins for structural analysis utilizing the LEXSY expression system

Daniil A. Kornilov, Sergey Bukhdruker, Fedor Tsybrov, Igor Chizhov et autres

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Mechanisms of inward transmembrane proton translocation

Kirill Kovalev, Fedor Tsybrov, Alexey Alekseev, Vitaly Shevchenko et autres

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Structural-phase states and micromechanical properties of nanostructured TiAlCuN coatings

С. В. Константинов, Ф. Ф. Комаров, Igor Chizhov, В. А. Зайков

TiAlCuN coatings were deposited by reactive magnetron sputtering on substrates of single-crystal silicon, and Titanium Grade2 wafers. To control and manage the coating deposition process by reactive magnetron sputtering, a previously developed modular gas flow control complex (MGFCC) was used. The elemental …

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Mirror proteorhodopsins

I.S. Okhrimenko, Kirill Kovalev, L. E. Petrovskaya, Nikolay S. Ilyinsky et autres

Proteorhodopsins (PRs), bacterial light-driven outward proton pumps comprise the first discovered and largest family of rhodopsins, they play a significant role in life on the Earth. A big remaining mystery was that up-to-date there was no described bacterial rhodopsins pumping protons at …

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Nanostructured TiAlCuN and TiAlCuCN coatings for spacecraft: effects of reactive magnetron deposition regimes and compositions

Ф. Ф. Комаров, С. В. Константинов, Igor Chizhov, В. А. Зайков et autres

Cu addition to the coating has effects on crystallite and growth column size refinement in comparison with the TiAlN and TiAlCN analogues due to its segregation along crystalline boundaries, and thus, imparts better mechanical characteristics.

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Influence of high-temperature ion irradiation on nanostructured TiAlN coatings

С. В. Константинов, Ф. Ф. Комаров, Igor Chizhov, J. Żuk et autres

Nanostructured TiAlN coatings were formed on AISI 304 stainless steel substrates by reactive magnetron sputtering. The studied TiAlN coatings were irradiated with Ar+ ions with an energy of 200 keV in the fluence range from 2.5 ⋅ 1016 to 2.0 ⋅ 1017 …

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Distinct actin–tropomyosin cofilament populations drive the functional diversification of cytoskeletal myosin motor complexes

Theresia Reindl, Sven Giese, Johannes N. Greve, P. Reinke et autres

The effects of N-terminal acetylation of the high molecular weight tropomyosin isoforms Tpm1.6 and Tpm2.1 and the low molecular weight isoforms Tpm1.12, Tpm3.1, and Tpm4.2 on the actin affinity and the thermal stability of actin-tropomyosin cofilaments are described. Furthermore, we show how …

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