Accès ouvert
2026
preprint
OpenAlex
Saša Jereb, Lia R. D’Alessandro, Nader Morshed, Margery Chen et autres
Abstract Microglia play key roles in brain development, homeostasis, and neurodegeneration. Although multiple strategies for viral gene delivery to microglia have been reported, they have not been directly compared. Here, we developed microglia-targeting AAV capsids and benchmarked them against existing approaches. The …
us
(code pays fourni par la source)
Accès ouvert
2026
article
OpenAlex
Nengsong Luo, Gao Y, Zengpeng Han, Peng Su et autres
Abstract Dual adeno-associated virus (AAV) vector reconstitution has become an indispensable technique for delivering large genes in neuroscience and gene therapy. A novel technology termed StitchR addresses the challenge of low efficiency in dual-AAV reconstruction, yet related tools enabling targeted gene delivery …
cn
(code pays fourni par la source)
Accès ouvert
2025
preprint
OpenAlex
Nengsong Luo, Yunling Gao, Zengpeng Han, Fuqiang Xu et autres
Abstract Understanding the mechanisms by which astrocytes regulate neural circuits is crucial for elucidating the mechanisms underlying cognitive and behavioral abnormalities in psychiatric disorders and for exploring potential therapeutic or intervention strategies. Viral tracers are powerful vehicles for comprehending the complex relationship …
cn, us
(code pays fourni par la source)
Accès ouvert
2025
article
OpenAlex
Zhu Gui, Kunzhang Lin, Nengsong Luo, Zengpeng Han et autres
decoding of neuronal activity and structural connectivity provides insights into brain structure-function interplay, which was important for understanding the cerebral function.
cn, us, fi
(code pays fourni par la source)
Accès ouvert
2025
article
OpenAlex
Nengsong Luo, Kunzhang Lin, Yuxiang Cai, Zilian Zhang et autres
The blood-brain barrier (BBB) is a formidable barrier that restricts the entry of substances into the brain, complicating the study of brain function and the treatment of neurological conditions. Traditional methods of delivering genes from the periphery to the central nervous system …
cn, us
(code pays fourni par la source)
Accès ouvert
2023
article
OpenAlex
Zengpeng Han, Nengsong Luo, Wenyu Ma, Xiaodong Liu et autres
Abstract Viral tracers that enable efficient retrograde labeling of projection neurons are powerful vehicles for structural and functional dissections of the neural circuit and for the treatment of brain diseases. Currently, some recombinant adeno-associated viruses (rAAVs) based on capsid engineering are widely …
cn, hk
(code pays fourni par la source)
Accès ouvert
2023
article
OpenAlex
Min Ying, Huadong Wang, Tongtan Liu, Zengpeng Han et autres
Herpes simplex virus type 1 (HSV-1) is a leading cause of encephalitis and infectious blindness. The commonly used clinical therapeutic drugs are nucleoside analogues such as acyclovir. However, current drugs for HSV cannot eliminate the latent virus or viral reactivation. Therefore, the …
cn
(code pays fourni par la source)
Accès ouvert
2023
article
OpenAlex
Zengpeng Han, Nengsong Luo, Fei Wang, Yuxiang Cai et autres
Adeno-associated viruses (AAVs) have become safe and effective tools for therapeutic in vivo gene drug delivery. Among many AAV serotypes, AAV2 is the most well-characterized. Although many studies have been carried out on the engineering of the capsid VR-VIII region, few attempts …
cn
(code pays fourni par la source)
Accès ouvert
2023
article
OpenAlex
Nengsong Luo, Zengpeng Han, Jiaxin Kou, Yuxiang Cai et autres
Rabies-viruses-based retrograde tracers can spread across multiple synapses in a retrograde direction in the nervous system of rodents and primates, making them powerful tools for determining the structure and function of the complicated neural circuits of the brain. However, they have some …
cn
(code pays fourni par la source)
Accès ouvert
2022
article
OpenAlex
Zengpeng Han, Nengsong Luo, Yang Wu, Jiaxin Kou et autres
As powerful tools for local gene delivery, adeno-associated viruses (AAVs) are widely used for neural circuit studies and therapeutical purposes. However, most of them have the characteristics of large diffusion range and retrograde labeling, which may result in off-target transduction during in …
cn
(code pays fourni par la source)
Accès ouvert
2022
article
OpenAlex
Zengpeng Han, Nengsong Luo, Jiaxin Kou, Lei Li et autres
Retrograde tracers based on viral vectors are powerful tools for the imaging and manipulation of upstream neural networks projecting to a specific brain region, and they play important roles in structural and functional studies of neural circuits. However, currently reported retrograde viral …
cn
(code pays fourni par la source)
Accès ouvert
2022
preprint
OpenAlex
Kunzhang Lin, Lei Li, Wenyu Ma, Xin Yang et autres
Abstract Neurotropic virus tracers, particularly those with low toxicity and high efficient tracing, are powerful tools for structural and functional dissections of neural circuits. The retrograde trans-mono-synaptic technology based on rabies virus CVS-N2c strain has reduced cytotoxicity and enhanced efficiency, attains long-term …
cn
(code pays fourni par la source)