Monique Le Ret

Expandable, Functional Hepatocytes Derived from Primary Cells Enable Liver Therapeutics

Sunil K. Mallanna, Soujanya S. Karanth, Joseph E. Marturano, Abhijith Kundadka Kudva et autres

Abstract Liver disease affects millions annually in the United States, with orthotopic transplantation as the only curative option for many patients. However, the scarcity of donor organs highlights a need for alternative cell-based therapies. Hepatocyte-based approaches are promising due to the cells’ …

RADA is a main branch migration factor in plant mitochondrial recombination and its defect leads to mtDNA instability and cell cycle arrest

Nicolas Chevigny, Frédérique Weber‐Lotfi, Cédric Nadiras, Arnaud Fertet et autres

ABSTRACT The mitochondria of flowering plants have large and complex genomes whose structure and segregation are modulated by recombination activities. The late steps of mitochondrial recombination are still poorly characterized: while the loss of mitochondrial recombination is not viable, a deficiency in …

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Efficient Replication of the Plastid Genome Requires an Organellar Thymidine Kinase

Monique Le Ret, Susan Belcher, Stéfanie Graindorge, Clémentine Wallet et autres

Thymidine kinase (TK) is a key enzyme of the salvage pathway that recycles thymidine nucleosides to produce deoxythymidine triphosphate. Here, we identified the single TK of maize (Zea mays), denoted CPTK1, as necessary in the replication of the plastidial genome (cpDNA), demonstrating …

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The RECG1 DNA Translocase Is a Key Factor in Recombination Surveillance, Repair, and Segregation of the Mitochondrial DNA in Arabidopsis

Clémentine Wallet, Monique Le Ret, Marc Bergdoll, Marc Bichara et autres

The mitochondria of flowering plants have considerably larger and more complex genomes than the mitochondria of animals or fungi, mostly due to recombination activities that modulate their genomic structures. These activities most probably participate in the repair of mitochondrial DNA (mtDNA) lesions …

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The RAD52-like protein ODB1 is required for the efficient excision of two mitochondrial introns spliced via first-step hydrolysis

José M. Gualberto, Monique Le Ret, Barbara Beator, Kristina Kühn

Transcript splicing in plant mitochondria involves numerous nucleus-encoded factors, most of which are of eukaryotic origin. Some of these belong to protein families initially characterised to perform unrelated functions. The RAD52-like ODB1 protein has been reported to have roles in homologous recombination-dependent …

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The RAD52-like protein ODB1 is required for the efficient excision of two mitochondrial introns spliced via first-step hydrolysis

José M. Gualberto, Monique Le Ret

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A RAD52‐like single‐stranded DNA binding protein affects mitochondrial DNA repair by recombination

Sabina Janicka, Kristina Kühn, Monique Le Ret, Géraldine Bonnard et autres

The plant mitochondrial DNA-binding protein ODB1 was identified from a mitochondrial extract after DNA-affinity purification. ODB1 (organellar DNA-binding protein 1) co-purified with WHY2, a mitochondrial member of the WHIRLY family of plant-specific proteins involved in the repair of organellar DNA. The Arabidopsis …

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RecA-Dependent DNA Repair Results in Increased Heteroplasmy of the Arabidopsis Mitochondrial Genome

Marie Miller-Messmer, Kristina Kühn, Marc Bichara, Monique Le Ret et autres

Plant mitochondria have very active DNA recombination activities that are responsible for its plastic structures and that should be involved in the repair of double-strand breaks in the mitochondrial genome. Little is still known on plant mitochondrial DNA repair, but repair by …

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A RAD52-like single-stranded DNA binding protein affects mitochondrial DNA repair by recombination.

S. Janicka, Kristina Kühn, Monique Le Ret, G. Bonnard et autres

International audience

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Arabidopsis tRNA Adenosine Deaminase Arginine Edits the Wobble Nucleotide of Chloroplast tRNAArg(ACG) and Is Essential for Efficient Chloroplast Translation

Étienne Delannoy, Monique Le Ret, Emmanuelle Faivre-Nitschke, Gonzalo M. Estavillo et autres

RNA editing changes the coding/decoding information relayed by transcripts via nucleotide insertion, deletion, or conversion. Editing of tRNA anticodons by deamination of adenine to inosine is used both by eukaryotes and prokaryotes to expand the decoding capacity of individual tRNAs. This limits …

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Arabidopsis tRNA adenosine deaminase arginine edits the wobble nucleotide of chloroplast tRNAArg(ACG) and is essential for efficient chloroplast translation.

Étienne Delannoy, Monique Le Ret, Emmanuelle Faivre-Nitschke, Gonzalo M. Estavillo et autres

International audience

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Degradation of a Polyadenylated rRNA Maturation By-Product Involves One of the Three RRP6-Like Proteins in Arabidopsis thaliana

Heike Lange, Sarah Holec, Valérie Cognat, Laurent Pieuchot et autres

Yeast Rrp6p and its human counterpart, PM/Scl100, are exosome-associated proteins involved in the degradation of aberrant transcripts and processing of precursors to stable RNAs, such as the 5.8S rRNA, snRNAs, and snoRNAs. The activity of yeast Rrp6p is stimulated by the polyadenylation …

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