Shigetoshi Sugio

CCDC 2183753: Experimental Crystal Structure Determination

Mitsunobu Doi, Toshimasa Ishida, Shigetoshi Sugio, Takashi Imagawa et autres

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, …

1999 XIII International Biophysics Congress 19-24 September 1999 New Delhi Abstracts Suppl

Narayanapillai Manoj, Vijay R. Srinivas, Avadhesha Surolia, Muthunanthevar Vijayan et autres

Bioinformatic identification of cytochrome b5 homologues from the parasitic nematode Ascaris suum and the free-living nematode Caenorhabditis elegans highlights the crucial role of A. suum adult-specific secretory cytochrome b5 in parasitic adaptation

Shinzaburo Takamiya, Muneaki Hashimoto, Toshihiro Mita, Takehiro Yokota et autres

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Cloning and Structure-Function Analyses of Quinolone- and Acridone-producing Novel Type III Polyketide Synthases from Citrus microcarpa

Takahiro Mori, Yoshihiko Shimokawa, Takashi Matsui, Keishi Kinjo et autres

Background: Type III polyketide synthases (PKSs) synthesize various polyketide and alkaloid scaffolds. Results: QNS synthesizes quinolone as the single product, whereas ACS produces acridone as the major product. Conclusion: QNS and ACS are novel quinolone- and acridone-producing type III PKSs, respectively. Significance: …

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Type III polyketide synthase

Tetsushi Mori, Yoshihiko Shimokawa, Takashi Matsui, Ryohei Kato et autres

Crystal structure of human Dlp1 in complex with GMP-PN.Pi

Hiroyuki Kishida, Shigetoshi Sugio

Crystal structure of GTPase domain fused with minimal stalks from human dynamin-1-like protein (Dlpl) in complex with several nucleotide analogues

Hiroyuki Kishida, Shigetoshi Sugio

Expression, purification and crystallization of an indole prenyltransferase fromAspergillus fumigatus

Jing Chen, Hiroyuki Morita, Ryohei Kato, Hiroshi Noguchi et autres

CdpNPT from Aspergillus fumigatus is a dimethylallyltryptophan synthase/indole prenyltransferase that catalyzes reverse prenylation at position N1 of tryptophan-containing cyclic dipeptides. Residues 38-440 of CdpNPT were expressed in Escherichia coli and crystallized using the sitting-drop vapour-diffusion and microseeding techniques. The crystals belonged to …

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Arg305 of Streptomyces l-glutamate oxidase plays a crucial role for substrate recognition

Tomohiro Utsumi, Jiro Arima, Chika Sakaguchi, Takashi Tamura et autres

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Synthesis of unnatural alkaloid scaffolds by exploiting plant polyketide synthase

Hiroyuki Morita, Makoto Yamashita, Shepo Shi, Toshiyuki Wakimoto et autres

HsPKS1 from Huperzia serrata is a type III polyketide synthase (PKS) with remarkable substrate tolerance and catalytic potential. Here we present the synthesis of unnatural unique polyketide-alkaloid hybrid molecules by exploiting the enzyme reaction using precursor-directed and structure-based approaches. HsPKS1 produced novel …

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Crystallization and preliminary X-ray analysis of 4-coumarate:CoA ligase fromArabidopsis thaliana

Hiroyuki Morita, Takahiro Mori, Kiyofumi Wanibuchi, Ryohei Kato et autres

4-Coumarate:CoA ligase 2 (4CL2) from Arabidopsis thaliana catalyzes the ATP-dependent formation of the 4-coumaroyl-CoA thioester through the formation of 4-coumarate-AMP. Recombinant 4CL2 protein was expressed in Escherichia coli and crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space group P2(1), …

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放線菌由来フラビン酵素L-グルタミン酸酸化酵素のユニークな構造(研究論文紹介)

二朗 有馬, Chiduko Sasaki, Chika Sakaguchi, Hiroshi Mizuno et autres