Gareth William Price

Connexin-43 hemichannels orchestrate NOD-like receptor protein-3 (NLRP3) inflammasome activation and sterile inflammation in tubular injury

Elena Roger, Christos Evangelos Chadjichristos, Panagiotis Kavvadas, Gareth William Price et autres

Abstract Background Without a viable cure, chronic kidney disease is a global health concern. Inflammatory damage in and around the renal tubules dictates disease severity and is contributed to by multiple cell types. Activated in response to danger associated molecular patterns (DAMPs) …

fr, gb (code pays fourni par la source)

Collagen I Modifies Connexin-43 Hemichannel Activity via Integrin α2β1 Binding in TGFβ1-Evoked Renal Tubular Epithelial Cells

Joe A. Potter, Gareth William Price, Chelsy L. Cliff, Colin Richard Green et autres

Chronic Kidney Disease (CKD) is associated with sustained inflammation and progressive fibrosis, changes that have been linked to altered connexin hemichannel-mediated release of adenosine triphosphate (ATP). Kidney fibrosis develops in response to increased deposition of extracellular matrix (ECM), and up-regulation of collagen …

gb, nz (code pays fourni par la source)

Danegaptide Prevents TGFβ1-Induced Damage in Human Proximal Tubule Epithelial Cells of the Kidney

Paul Edward Squires, Gareth William Price, Ulrik Mouritzen, Joe A. Potter et autres

Chronic kidney disease (CKD) is a global health problem associated with a number of comorbidities. Recent evidence implicates increased hemichannel-mediated release of adenosine triphosphate (ATP) in the progression of tubulointerstitial fibrosis, the main underlying pathology of CKD. Here, we evaluate the effect …

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Carboxyfluorescein Dye Uptake to Measure Connexin-mediated Hemichannel Activity in Cultured Cells

Joe A. Potter, Gareth William Price, Chelsy L. Cliff, Bethany M. Williams et autres

Connexins are membrane bound proteins that facilitate direct and local paracrine mediated cell-to-cell communication through their ability to oligomerise into hexameric hemichannels. When neighbouring channels align, they form gap-junctions that provide a direct route for information transfer between cells. In contrast to …

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Inhibition of intern A2B1 negates collagen-1 induced changes in connexion-mediated hemichannel activity in an in vitro model of diabetic nephropathy

Joe A. Potter, Gareth William Price, Chelsy L. Cliff, Bethany M. Williams et autres

Aims: Tubulointerstitial fibrosis, the underlying pathology of diabetic nephropathy, is characterised by extracellular matrix accumulation. Aberrant connexin (Cx) expression and increased adenosine triphosphate (ATP) have been linked to inflammation and fibrosis, and we have previously reported that glucose-evoked transforming growth factor-beta1 (TGF-b1) …

A role for connexin-43 hemichannel-mediated adenosine triphosphate triphosphate (ATP) release in inflammation of the diabetic proximal tubule

Gareth William Price, Joe A. Potter, Bethany M. Williams, Chelsy L. Cliff et autres

Aim: Glucose-evoked transforming growth factor beta1 (TGF-b1) dysregulates connexin (Cx)-mediated gap-junction intercellular communication and increases hemichannel adenosine triphosphate (ATP) release. Linked with inflammation in diabetes, we investigated a role for ATP in activation of the NLRP3 inflammasome and production of inflammatory mediators …

Blocking Connexin-43 mediated hemichannel activity protects against early tubular injury in experimental chronic kidney disease

Gareth William Price, Christos Evangelos Chadjichristos, Panagiotis Kavvadas, Sydney C.W. Tang et autres

Abstract Background Tubulointerstitial fibrosis represents the key underlying pathology of Chronic Kidney Disease (CKD), yet treatment options remain limited. In this study, we investigated the role of connexin43 (Cx43) hemichannel-mediated adenosine triphosphate (ATP) release in purinergic-mediated disassembly of adherens and tight junction …

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Examining Local Cell-to-Cell Signalling in the Kidney Using ATP Biosensing

Gareth William Price, Joe A. Potter, Bethany M. Williams, Chelsy L. Cliff et autres

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Connexin‐mediated cell communication in the kidney: A potential therapeutic target for future intervention of diabetic kidney disease?

Gareth William Price, Joe A. Potter, Bethany M. Williams, Chelsy L. Cliff et autres

The ability of cells to communicate and synchronise their activity is essential for the maintenance of tissue structure, integrity and function. A family of membrane-bound proteins called connexins are largely responsible for mediating the local transfer of information between cells. Assembled in …

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Purinergic receptor (P2X7) activation reduces cell–cell adhesion between tubular epithelial cells of the proximal kidney

Eleftherios Siamantouras, Gareth William Price, Joe A. Potter, Claire Elizabeth Hills et autres

Loss of epithelial (E)-cadherin mediated cell-cell adhesion impairs gap junction formation and facilitates hemichannel-mediated ATP release in the diabetic kidney. Linked to inflammation and fibrosis, we hypothesized that local increases in inter-cellular ATP activate P2X7 receptors on neighboring epithelial cells of the …

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ATP reduces functional cell-to-cell tethering between renal tubular epithelial cells

Paul Edward Squires, Eleftherios Siamantouras, Joe A. Potter, Gareth William Price et autres

Background: Loss of epithelial (E)-cadherin mediated cell-cell adhesion impairs gap junction formation and facilitates hemichannel-mediated ATP release in the diabetic kidney. Linked to inflammation and fibrosis, we hypothesize that local increases in inter-cellular ATP activate P2X7 receptors on neighbouring epithelial cells of …

Purinergic receptor (P2X7) activation contributes to disassembly of adherens & tight junctions in tubular epithelial cells of the diabetic kidney.

Gareth William Price, Eleftherios Siamantouras, Joe A. Potter, Paul Edward Squires et autres