Gat Rauner

Human Milk as a Model for Next-Generation Infant Formula: Opportunities and Challenges

Marie R.Biondi Ryan, Clay Swackhamer, Sharon M. Donovan, Jennifer T. Smilowitz et autres

Human milk (HM) is a dynamic biological system replete with functional components that support infant growth, development and immune protection. HM is regarded as the optimal infant food; however, many infants rely on infant formula (IF) as their sole source of nutrition …

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A Defined Hydrogel-Based Method For Generating Three-Dimensional Human Breast Organoids That Recapitulate Mammary Morphogenesis

Nicole Traugh, Meloryn Seraj, Gat Rauner, Charlotte Kuperwasser

The development of physiologically relevant human model systems that recapitulate tissue architecture and cell-state dynamics remains a major challenge in studying breast development and early events in carcinogenesis. Conventional two-dimensional cultures and many three-dimensional systems fail to capture the structural organization and …

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Correction: Single-cell organogenesis captures complex breast tissue formation in three dimensions

Gat Rauner, Nicole C. Traugh, Colin J. Trepicchio, Meadow E. Parrish et autres

There were errors in Development (2025) 152, dev204813 (doi:10.1242/dev.204813).The authors contacted us to say that there were three incorrectly cited references. The updated citations are detailed below.In the first paragraph of the ‘Results: Regeneration, architecture and patterning of patient-derived organoids from single …

Single-cell organogenesis captures complex breast tissue formation in three dimensions

Gat Rauner, Nicole C. Traugh, Colin J. Trepicchio, Meadow E. Parrish et autres

Current breast organoid models, primarily based on self-assembly, face limitations in accurately mimicking the complex stages of tissue development, notably in replicating detailed tissue architectures and cellular diversity. Here, we demonstrate that a solitary human breast stem cell can, within a controlled …

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From 2D to 3D and beyond: the evolution and impact of in vitro tumor models in cancer research

Gat Rauner, Piyush B. Gupta, Charlotte Kuperwasser

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DDR1 regulates RUNX1-CBFβ to control breast stem cell differentiation

Colin Trepicchio, Gat Rauner, Nicole C. Traugh, Ruohong Wang et autres

Understanding epithelial stem cell differentiation and morphogenesis during breast tissue development is essential, as disruption in these processes underlie breast cancer formation. We used a next-generation single-cell-derived organoid model to investigate how individual stem cells give rise to complex tissue. We show …

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Immune-Epithelial Interactions via TGF-β Orchestrates Stem-Cell Niche Formation and Morphogenesis

Nicole C. Traugh, Colin Trepicchio, Gat Rauner, Meadow E. Parrish et autres

Abstract Mesenchymal-epithelial interactions are well-established for organogenesis, but the role of immune cells in these processes remains less explored. Organoids offer powerful models for studying tissue development, especially where in vivo models are limited. In this study, we established an immune-epithelial co-culture …

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Next-Generation Breast Organoids Capture Human Organogenesis With High-Resolution Live Imaging

Gat Rauner, Nicole C. Traugh, Colin J. Trepicchio, Meadow E. Parrish et autres

Organoids have emerged as a powerful tool for modeling tissue growth and diseases. In this study, we introduce a groundbreaking organotypic culture technique that replicates the morphology, scale, and heterogeneity of human breast tissue, and includes a mesenchymal-like stromal component. A standout …

Mammary glands in a dish − what miniature organs reveal about evolution, lactation, regeneration and breast cancer

Gat Rauner

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Using Organoids to Tap Mammary Gland Diversity for Novel Insight

Gat Rauner

This article offers a comprehensive perspective on the transformative role of organoid technology on mammary gland biology research across a diverse array of mammalian species.The mammary gland's unique development and regenerative capabilities render this organ an ideal model for studying developmental evolution, …

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DDR1 regulates RUNX1-CBFβ to control breast stem cell differentiation

Colin Trepicchio, Gat Rauner, Nicole C. Traugh, Meadow E. Parrish et autres

Abstract The human breast is complex and comprised of multi-lineage and multi-structural elements. Recent work has shown that epithelial stem and progenitor cells use the collagen receptor Discoidin Domain Receptor 1 (DDR1) for differentiation into both basal and luminal cell lineages, which …

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Expanding the evo-devo toolkit: generation of 3D mammary tissue from diverse mammals

Hahyung Y. Kim, Ishani Sinha, Karen E. Sears, Charlotte Kuperwasser et autres

The varying pathways of mammary gland development across species and evolutionary history are underexplored, largely due to a lack of model systems. Recent progress in organoid technology holds the promise of enabling in-depth studies of the developmental adaptations that have occurred throughout …

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