Sunil Kumar

Archive for "Condenserless polarisation differential phase contrast microscopy for high resolution quantitative phase imaging"

Huihui Liu, Sunil Kumar, Edwin García, Qianying Yan et autres

This is the archive for the manuscript "Condenserless polarisation differential phase contrast microscopy for high resolution quantitative phase imaging". This archive includes: Data: raw data, processing code, and processed data Hardware: parts list, design files, and assembly instructions Software: compiled JAR file …

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Archive for "Condenserless polarisation differential phase contrast microscopy for high resolution quantitative phase imaging"

Huihui Liu, Sunil Kumar, Edwin García, Qianying Yan et autres

This is the archive for the manuscript "Condenserless polarisation differential phase contrast microscopy for high resolution quantitative phase imaging". This archive includes: Data: raw data, processing code, and processed data Hardware: parts list, design files, and assembly instructions Software: compiled JAR file …

gb (code pays fourni par la source)

Quantitative evaluation of LED based optical autofocus module

Sara Habte, Sunil Kumar, Jonathan Lightley, Edwin Garcia et autres

Abstract We report an improved version of the open-source optical autofocus module (“ openAF ”) for light microscopy using a light emitting diode (LED), together with a method to independently quantify the performance of optical autofocus systems using 2D autocorrelation analysis of …

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Abstract A038: Monitoring PARP trapping in live ovarian cancer cells using a CRISPR-engineered FRET biosensor

Edwin García, Sunil Kumar, Katherine Tyson, Caroline Koch et autres

Abstract Poly(ADP-ribose) polymerase inhibitors (PARPi) have revolutionized treatment for high-grade serous ovarian carcinoma (HGSOC), particularly in homologous recombination-deficient (HRD) tumors. However, over half of patients relapse within four years, and acquired resistance remains a major barrier to durable clinical benefit. A key …

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PolSpec : Polarisation‐Based Detection for Versatile, Cost‐Effective Rapid Hyperspectral Imaging

Huihui Liu, Sunil Kumar, Edwin García, Darren Ennis et autres

"PolSpec" is a flexible, cost-effective approach for rapid (including single-shot) spectrally resolved imaging. While established approaches, e.g., using cascades of dichroic beamsplitters, diffractive image splitters, or mosaic filters, typically have pre-determined spectral detection bands with cost and experimental complexity scaling with the …

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Assessing PARP trapping dynamics in ovarian cancer using a CRISPR-engineered FRET biosensor

D I Marks, Edwin García, Sunil Kumar, Katie Tyson et autres

Abstract Poly(ADP-ribose) polymerase inhibitors (PARPi) have revolutionised the treatment of ovarian high grade serous carcinoma (HGSC), especially those with defective homologous recombination. However, the emergence of resistance poses a critical challenge, as over 50% of patients relapse within three years. The mechanisms …

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Elucidating a Mechanistic Role of L-PGDS in Obesity Using 3T3-L1 Pre-Adipocytes (Abstract ID: 169840)

Marjia Sultana, Sunil Kumar

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Open-source implementation of polarisation-resolved single-shot differential phase contrast microscopy (pDPC) on a modular openFrame-based microscope

Huihui Liu, Sunil Kumar, Edwin García, William Flanagan et autres

We recently demonstrated polarisation differential phase contrast microscopy ( pDPC ) as a robust, low-cost single-shot implementation of (semi)quantitative phase imaging based on differential phase microscopy. pDPC utilises a polarisation-sensitive camera to simultaneously acquire four obliquely transilluminated images from which phase images …

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PolSpec : polarisation-based detection for spectral classification of optical signals

Huihui Liu, Sunil Kumar, Edwin García, P. M. W. French

Abstract Spectrally resolved imaging is typically realised using bandpass filters, which are inefficient when they reject “out-of-band” photons or using angularly dispersive devices with at least one image dimension requiring scanning to acquire a full hyperspectral dataset and therefore sequential data acquisition, …

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Super-resolved fluorescence imaging utilising accessible stochastic optical reconstruction microscopy (easySTORM) implemented on a low-cost, modular open-source (openFrame) microscope

Anupam Bharadwaj, Amalesh Kumar, Ranjan Kalita, Rumela Mitra et autres

Abstract Optical super-resolution microscope is a powerful tool for the life sciences, including cell biology and pathology yielding information on, e.g., biological structure and protein distribution in the nano-scale range. In recent years, there has been an exponential rise in the interest …

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Dissecting the novel anti-obesity mechanism of prostaglandin D2 via DP1 receptor agonism utilizing mouse 3T3-L1 adipocytes as well as primary human subcutaneous adipocytes

Mehonaz Sultana, Sunil Kumar

ie (code pays fourni par la source)

Environmentally dependent and independent control of 3D cell shape

Lucas Dent, Nathan Curry, Hugh Sparks, Vicky Bousgouni et autres

How cancer cells determine their shape in response to three-dimensional (3D) geometric and mechanical cues is unclear. We develop an approach to quantify the 3D cell shape of over 60,000 melanoma cells in collagen hydrogels using high-throughput stage-scanning oblique plane microscopy (ssOPM). …

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