Archive for "Condenserless polarisation differential phase contrast microscopy for high resolution quantitative phase imaging"

Le résumé fourni par la source

This is the archive for the manuscript "Condenserless polarisation differential phase contrast microscopy for high resolution quantitative phase imaging". This archive includes: Data: raw data, processing code, and processed data Hardware: parts list, design files, and assembly instructions Software: compiled JAR file and source code Article Abstract: Polarization differential phase contrast microscopy (pDPC) is a robust, low-cost single-shot implementation of quantitative phase imaging based on differential phase contrast (DPC) microscopy utilising a polarization-sensitive camera to simultaneously acquire four obliquely illuminated images. We recently reported a cost-effective open-source implementation of pDPC on a modular “openFrame-based” microscope, where the spatial resolution was limited by the numerical aperture (NA) of the condenser lens, as is generally the case for condenser-based DPC. Here we present even lower-cost open-source implementations that overcome this limitation by using domestic lighting illumination sources to realise quantitative phase imaging (QPI) at up to ~0.95 NA. We have applied these pDPC implementations to image mammalian cells and mycobacteria.

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