Péter Ecsédi

SSB1 links stress granule regulation to cellular stress responses and renal ischemia-reperfusion injury

János Pálinkás, Bálint Jezsó, Eszter Nagy‐Kanta, Rita Nemeth et autres

SUMMARY Mammalian single-stranded DNA binding protein 1 (SSB1) has been established as an essential component of genome stability in both human cells and mice. Moreover, SSB1 was recently implicated in cytoplasmic stress response by its involvement in Ras GTPase-activating protein-binding protein 1 …

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Phase transition of bacterial single-stranded DNA binding (SSB) protein upon stress response and metabolic adaptation

Péter Ecsédi, Júlia Szittyai, János Pálinkás, Bálint Jezsó et autres

Abstract Single-stranded DNA-binding proteins (SSBs) are ubiquitous factors of genome metabolism, recently recognized for their ability to undergo liquid–liquid phase separation (LLPS). While Escherichia coli SSB (EcSSB) has emerged as a model for bacterial LLPS in vitro , its phase behavior and …

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Selective engineering of condensation properties of single-stranded DNA binding (SSB) protein via its intrinsically disordered linker region

Péter Ecsédi, Dávid Érfalvy, Zoltán J. Kovács, Viktoria Katran et autres

Single-stranded DNA binding (SSB) proteins are essential components of genome metabolism in both bacteria and eukaryotes. Recently demonstrated condensation propensities have placed SSB functions in a new context regarding the organization of nucleic acid-modifying complexes. In this work, we provide functional dissection …

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Fine‐tuned interactions between globular and disordered regions of single‐stranded DNA binding ( SSB ) protein are required for dynamic condensation under physiological conditions

Zoltán J. Kovács, Péter Ecsédi, Gábor M. Harami, János Pálinkás et autres

Abstract Increasing evidence points to the importance of liquid–liquid phase separation (LLPS)‐driven protein condensation in both eukaryotic and bacterial cell physiology. The formation of condensates may involve interactions between both structured (globular) domains and intrinsically disordered protein regions and requires multivalency that …

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Evaluation of an Affinity-Enhanced Anti-SARS-CoV2 Nanobody Design Workflow Using Machine Learning and Molecular Dynamics

Zsolt Fazekas, Dóra Nagy‐Fazekas, Boglárka Mária Shilling-Tóth, Péter Ecsédi et autres

High Resolution Image Download MS PowerPoint Slide In silico optimization of protein binding has received a great deal of attention in the recent years. Since in silico prefiltering of strong binders is fast and cheap compared to in vitro library screening methods, …

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Functional contribution of condensate formation by single-stranded DNA binding protein (SSB) to bacterial genome maintenance

Péter Ecsédi, János Pálinkás, Zoltán J. Kovács, Gábor M. Harami et autres

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Redox-dependent phase separation and cytoplasmic granulation by human single-stranded DNA binding protein 1 (HSSB1) point to new mechanisms of cellular stress response

János Pálinkás, Gábor M. Harami, Zoltán J. Kovács, Bálint Jezsó et autres

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Assignment of the disordered, proline-rich N-terminal domain of the tumour suppressor p53 protein using 1HN and 1Hα-detected NMR measurements

Fanni Sebák, Péter Ecsédi, László Nyitray, Andrea Bodor

Abstract Protein p53 is mostly known for playing a key role in tumour suppression, and mutations in the p53 gene are amongst the most frequent genomic events accompanying oncogenic transformation. Continuous research is conducted to target disordered proteins/protein regions for cancer therapy, …

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A Novel Fusion Protein System for the Production of Nanobodies and the SARS-CoV-2 Spike RBD in a Bacterial System

Dóra Nagy‐Fazekas, Pál Stráner, Péter Ecsédi, Nóra Taricska et autres

Antibodies are key proteins of the immune system, and they are widely used for both research and theragnostic applications. Among them, camelid immunoglobulins (IgG) differ from the canonical human IgG molecules, as their light chains are completely missing; thus, they have only …

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A scalable strategy to solve structures of PDZ domains and their complexes

A. Cousido-Siah, Laura Carneiro, Camille Kostmann, Péter Ecsédi et autres

The human PDZome represents one of the largest globular domain families in the human proteome, with 266 instances. These globular domains typically interact with C-terminal peptide motifs found in thousands of human proteins. Despite previous efforts, not all PDZ domains have experimentally …

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Studying the Structures of Relaxed and Fuzzy Interactions: The Diverse World of S100 Complexes

Péter Ecsédi, Gergő Gógl, László Nyitray

S100 proteins are small, dimeric, Ca2+-binding proteins of considerable interest due to their associations with cancer and rheumatic and neurodegenerative diseases. They control the functions of numerous proteins by forming protein–protein complexes with them. Several of these complexes were found to display …

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Selective1HαNMR Methods Reveal Functionally Relevant Prolinecis/transIsomers in Intrinsically Disordered Proteins: Characterization of Minor Forms, Effects of Phosphorylation, and Occurrence in Proteome

Fanni Sebák, Péter Ecsédi, Wolfgang Bermel, Burkhard Luy et autres

Abstract It is important to identify prolinecis/transisomers that appear in several regulatory mechanisms of proteins, and to characterize minor species that are present due to the conformational heterogeneity in intrinsically disordered proteins (IDPs). To obtain residue level information on these mobile systems …

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