Mitchell R. White

Harnessing Hazara Virus as a Surrogate for Crimean–Congo Hemorrhagic Fever Virus Enables Inactivation Studies at a Low Biosafety Level

Judith Olejnik, Kristina Meier, Javier Vargas-Herrera, Daniel J. DeStasio et autres

Research on highly pathogenic biosafety level 4 (BSL-4) viruses that are classified as Select Agents involves transferring inactivated materials to lower containment levels for further analysis. Compliance with Select Agent and BSL-4 safety regulations necessitates the validation and verification of inactivation procedures. …

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Heat Inactivation of Nipah Virus for Downstream Single-Cell RNA Sequencing Does Not Interfere with Sample Quality

Adam J. Hume, Judith Olejnik, Mitchell R. White, Jessie Huang et autres

Single-cell RNA sequencing (scRNA-seq) technologies are instrumental to improving our understanding of virus-host interactions in cell culture infection studies and complex biological systems because they allow separating the transcriptional signatures of infected versus non-infected bystander cells. A drawback of using biosafety level …

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Calreticulin Regulates SARS-CoV-2 Spike Protein Turnover and Modulates SARS-CoV-2 Infectivity

Nader Rahimi, Mitchell R. White, Razie Amraei, Saran Lotfollahzadeh et autres

Cardiovascular complications are major clinical hallmarks of acute and post-acute coronavirus disease 2019 (COVID-19). However, the mechanistic details of SARS-CoV-2 infectivity of endothelial cells remain largely unknown. Here, we demonstrate that the receptor binding domain (RBD) of the SARS-CoV-2 spike (S) protein …

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Art of the Kill: Designing and Testing Viral Inactivation Procedures for Highly Pathogenic Negative Sense RNA Viruses

Judith Olejnik, Adam J. Hume, Stephen J. Ross, Whitney A. Scoon et autres

The study of highly pathogenic viruses handled under BSL-4 conditions and classified as Select Agents frequently involves the transfer of inactivated materials to lower containment levels for downstream analyses. Adhering to Select Agent and BSL-4 safety regulations requires validation or verification of …

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Support for Recommendations of Precleaning Before Disinfection: Limited Efficacy of Soapy Water as a Disinfectant against SARS-CoV-2

Gabrielle M. String, Yarmina Kamal, Mitchell R. White, David M. Gute et autres

The World Health Organization (WHO) and US Centers for Disease Control and Prevention (CDC) recommend cleaning soiled surfaces with soap and water, followed by use of approved disinfectant. However, data are lacking on the potential efficacy of soapy water alone as a …

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Correction: Recombinant Lloviu virus as a tool to study viral replication and host responses

Adam J. Hume, Baylee Heiden, Judith Olejnik, Ellen L. Suder et autres

The x-axis label for panel D of Fig 5 is incorrect.The label should be: mAb114 concentration (μg/mL).The authors have provided a corrected version of Fig 5 here.

Recombinant Lloviu virus as a tool to study viral replication and host responses

Adam J. Hume, Baylee Heiden, Judith Olejnik, Ellen L. Suder et autres

Next generation sequencing has revealed the presence of numerous RNA viruses in animal reservoir hosts, including many closely related to known human pathogens. Despite their zoonotic potential, most of these viruses remain understudied due to not yet being cultured. While reverse genetic …

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Extracellular vimentin is an attachment factor that facilitates SARS-CoV-2 entry into human endothelial cells

Razie Amraei, Chaoshuang Xia, Judith Olejnik, Mitchell R. White et autres

SARS-CoV-2 entry into host cells is a crucial step for virus tropism, transmission, and pathogenesis. Angiotensin-converting enzyme 2 (ACE2) has been identified as the primary entry receptor for SARS-CoV-2; however, the possible involvement of other cellular components in the viral entry has …

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Viral Evasion of Innate Immune Defense: The Case of Resistance of Pandemic H1N1 Influenza A Virus to Human Mannose-Binding Proteins

Mitchell R. White, Nikolaos M. Nikolaidis, Francis X. McCormack, Erika C. Crouch et autres

Mannose-binding lectins effectively inhibit most seasonal strains of influenza A virus and contribute to the innate host defense vs. these viruses. In contrast, pandemic IAV strains are largely resistant to these lectins, likely contributing to increased spread and worse outcomes. In this …

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Selection of a SARS-CoV-2 Surrogate for Use in Surface Disinfection Efficacy Studies with Chlorine and Antimicrobial Surfaces

Gabrielle M. String, Mitchell R. White, David M. Gute, Elke Mühlberger et autres

Initial recommendations for surface disinfection to prevent SARS-CoV-2 transmission were developed using previous evidence from potential surrogates. To the best of our knowledge, no appropriate surrogate for SARS-CoV-2 has been identified or confirmed for chlorine and antimicrobial surface disinfection. We completed a …

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DECOLONIZING CANCER CARE IN CANADA

Matthew Beckett, Katherine Marie Cole, Mitchell R. White, Jessica Chan et autres

Cancer incidence and mortality among Indigenous peoples of Canada (First Nations, Inuit, and Métis) continue to rise in contrast to non-Indigenous Canadians, and Indigenous peoples are at higher risk of cancers associated with known modifiable risk factors. Jurisdictional and administrative challenges have …

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Recombinant Lloviu virus as a model to study inaccessible zoonotic viruses

Adam J. Hume, Baylee Heiden, Judith Olejnik, Ellen L. Suder et autres

Abstract Next generation sequencing has revealed the presence of many RNA viruses in animal reservoir hosts, including many closely related to known human pathogens. Despite their zoonotic potential, many of these viruses remain understudied due to not yet being cultured. While reverse …

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