Jian Wen

DMSO/Tf 2 O-promoted regio- and Z -stereoselective sulfonyl functionalization of terminal alkynes

Yuxin Guo, Hanru Tang, Zhiyan Liu, ZhiPing WANG et autres

We report a DMSO/Tf 2 O-promoted regio- and Z -stereoselective sulfonyl functionalization of terminal alkynes that delivers diverse functionalized vinyl sulfones in a highly selective manner under tunable basic conditions.

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Nickel-Catalyzed Desulfurizative Sonogashira Cross-Coupling of Terminal Alkynes with Alkenylsulfonium Salts

Mengke Su, Xiaoyu Li, Mingchuan Zhang, Jian Wen

A new protocol for C(sp 2 )–C(sp) bond formation via the nickel-catalyzed desulfurizative Sonogashira cross-coupling reaction involving C(sp 2 )–S bond cleavage with alkenylsulfonium salts as an electrophilic coupling partner is described. The reaction proceeded smoothly for preparation of ( E )-conjugated …

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Enzymatic C N bond formation and cleavage reaction: Advances and perspectives

Yaoyun Wu, Wei Song, Wanqing Wei, Jian Wen et autres

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Sulfur-Mediated Aminothiolation of Terminal Alkynes: Regioselective Synthesis of Z-Trisubstituted Vinyl Sulfides

Long‐Yong Xie, Ziyu Wang, Jian Wen

The first sulfur-mediated aminothiolation of terminal alkynes through a one-pot procedure under mild conditions has been established. This method is practical, easy to manipulate, and exhibits a wide substrate range with good functional group tolerance, resulting in Z -selective trisubstituted vinyl sulfides …

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Stereoselective Biosynthesis of Bichiral Amino Alcohols from Diketone via Difunctionalization

Tianfu Wu, Wei Song, Jing Wu, Jian Wen et autres

High Resolution Image Download MS PowerPoint Slide Chiral amino alcohols are vital in many biologically active molecules and natural products and are crucial building blocks for the pharmaceutical industry. However, the chemosynthesis of chiral amino alcohols, particularly bichiral amino alcohols, faces challenges …

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Mechanism‐Guided Protein Engineering of Paraburkholderia xenovorans Lactamase for the Green Synthesis of 2‐(2‐Oxopyrrolidin‐1‐yl)‐Butanoic Acid

Witz Ma, Wanqing Wei, Jian Wen, Cong Gao et autres

The synthesis of tertiary amides is crucial in pharmaceutical manufacturing, but achieving this efficiently through enzymatic methods remains challenging. Here, we describe the screening, evolution, and application of a lactamase for the synthesis of 2-(2-oxopyrrolidin-1-yl)-butanoic acid (2-OYBA) in aqueous phase. Through database …

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Semirational Design of 4-Hydroxyphenylpyruvate Dioxygenase for Efficient and Regioselective Production of 3-Hydroxy-3-methylbutyrate

Yihan Chen, Wanqing Wei, Jianhao Xu, Wei Song et autres

3-Hydroxy-3-methylbutyrate (HMB), an important dietary supplement, can enhance the quality of life for sedentary and elderly people. However, the catalytic efficiency of the pathway enzymes limited the biosynthesis of HMB. Here, we identified an efficient 4-hydroxyphenylpyruvate dioxygenase (4-HPPD) from Bacteroides barnesiae ( …

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Fusing Engineered CYP109E1 and a New Reductase Domain for 25(OH)VD 3 Biosynthesis

Xisong Feng, Wanqing Wei, Wei Song, Jian Wen et autres

25-hydroxyvitamin D 3 (25(OH)VD 3 ) is an important daily nutritional supplement, and direct enzymatic C25 hydroxylation of vitamin D 3 (VD 3 ) to 25(OH)VD 3 is a green and sustainable method. However, the low catalytic activity and electron transfer efficiency …

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Thermostable Coenzyme a Ligase for Efficient Biosynthesis of 2-Pyrrolidone via Protein and Fermentation Engineering

Bei Yu, Wei Song, Wanqing Wei, Guipeng Hu et autres

2-Pyrrolidone is an important chemical intermediate with broad applications in the materials and pharmaceutical industries. Traditional petrochemical synthesis methods pose significant environmental challenges. In the case of biosynthesis, the limited thermostability of coenzyme A ligase (CaiC) represents a major barrier to industrial-scale …

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Reshaping Interface Interactions of P. litoralis Acyltransferase for Efficient Chemoenzymatic Epoxidation in Aqueous Phase

Zihao Yin, Wanqing Wei, Wei Song, Jian Wen et autres

Epoxides, a class of ethers with a three-membered ring structure, are widely used in the textile, pharmaceutical, and packaging industries. Chemoenzymatic epoxidation presents a promising method for synthesizing epoxides. However, its epoxidation efficiency is hindered by low chemoselective perhydrolysis, which is caused …

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Construction of Escherichia coli Cell Factory for Efficient Synthesis of Indigo

Shiqi Luo, Wei Song, Guipeng Hu, Jian Wen et autres

Abstract Indigo is widely used in dyes, medicines and semiconductors materials due to its excellent dyeing efficiency, antibacterial, antiviral, anticancer, anti‐corrosion, and thermostability properties. Here, a biosynthetic pathway for indigo was designed, integrating two enzymes (EcTnaA, MaFMO) into a higher L‐tryptophan‐producing the …

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Efficient Synthesis of l -Asparagine by an Immobilized Three-Enzyme Cascade Reaction System

Ran Wang, Wei Song, Huimin Xu, Jian Wen et autres

l -Asparagine ( l -Asn) is an important amino acid with broad applications in food, medicine, fine chemicals, and environmental protection. However, its industrial production is limited by the high cost of raw materials and the low catalytic efficiency of enzymes. In …

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