Horacio M. Pallarés

The RNA promoter for pathogenic orthoflaviviruses replication is universal and serves as target for viral inhibition

Santiago Oviedo Rouco, Lautaro Bertoni, Evelyn Mikkelsen, Carolina Sarto et autres

Orthoflaviviruses comprise a diverse genus of positive-strand RNA viruses that includes major human pathogens such as dengue, yellow fever, Zika, Japanese encephalitis, tick-borne encephalitis, and West Nile viruses. Despite their global impact, the molecular constraints that preserve viral replication across distinct vectors …

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A conserved RNA structure at the capsid-coding sequence of Zika virus genome is required for viral replication in a host-dependent manner

Guadalupe S. Costa Navarro, Horacio M. Pallarés, María Mora González López Ledesma, Luana de Borba et autres

Flaviviruses are emerging and re-emerging pathogens causing widespread epidemics worldwide. Their RNA genomes play multiple roles during infection, folding into dynamic structures that regulate viral processes. To understand the mechanisms of flavivirus infection and to design genetic tools for viral countermeasures, it …

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Versatile enhancement of the killing potential of anti-cancer agents achieved by peptide mimetics of the PCNA interface towards specialized DNA polymerases

Yiovana Verónica Okraine, María Belén de la Vega, Sofía Venerus Arbilla, Ginette Moyano et autres

Cancer cells that survive chemotherapy achieve full DNA duplication despite the accumulation of damaged DNA triggered by chemotherapy. This happens because the synthesis of DNA at damaged sites is granted by tolerance events including translesion DNA synthesis (TLS), a process that promotes …

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Overcoming Limited Access to Virus Infection Rapid Testing: Development of a Lateral Flow Test for SARS-CoV-2 with Locally Available Resources

Estefanía S. Peri Ibáñez, Agostina Mazzeo, Carolina Silva, Maria Juliana Juncos et autres

The COVID-19 pandemic highlighted testing inequities in developing countries. Lack of lateral flow test (LFT) manufacturing capacity was a major COVID-19 response bottleneck in low- and middle-income regions. Here we report the development of an open-access LFT for SARS-CoV-2 detection comparable to …

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Dengue virus preferentially uses human and mosquito non-optimal codons

Luciana Andrea Castellano, Ryan J McNamara, Horacio M. Pallarés, Andrea Vanesa Gamarnik et autres

Codon optimality refers to the effect that codon composition has on messenger RNA (mRNA) stability and translation level and implies that synonymous codons are not silent from a regulatory point of view. Here, we investigated the adaptation of virus genomes to the …

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Zika virus non-coding RNAs antagonize antiviral responses by PKR-mediated translational arrest

Horacio M. Pallarés, María Mora González López Ledesma, Santiago Oviedo Rouco, Luciana Andrea Castellano et autres

Zika virus (ZIKV) is an emerging mosquito-borne flavivirus that causes severe outbreaks in human populations. ZIKV infection leads to the accumulation of small non-coding viral RNAs (known as sfRNAs) that are crucial for evasion of antiviral responses and for viral pathogenesis. However, …

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Dengue virus preferentially uses human and mosquito non-optimal codons

Luciana Andrea Castellano, Ryan J McNamara, Horacio M. Pallarés, Andrea Vanesa Gamarnik et autres

Abstract Codon optimality refers to the effect codon composition has on messenger RNA (mRNA) stability and translation level and implies that synonymous codons are not silent from a regulatory point of view. Here, we investigated the adaptation of virus genomes to the …

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Dengue virus NS5 degrades ERC1 during infection to antagonize NF-kB activation

María Mora González López Ledesma, Guadalupe S. Costa Navarro, Horacio M. Pallarés, Ana Luz Paletta et autres

Dengue virus (DENV) is the most important human virus transmitted by mosquitos. Dengue pathogenesis is characterized by a large induction of proinflammatory cytokines. This cytokine induction varies among the four DENV serotypes (DENV1 to 4) and poses a challenge for live DENV …

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Longitudinal Study after Sputnik V Vaccination Shows Durable SARS-CoV-2 Neutralizing Antibodies and Reduced Viral Variant Escape to Neutralization over Time

María Mora González López Ledesma, Lautaro Sánchez, Diego Sebastian Ojeda, Santiago Oviedo Rouco et autres

Vaccines have been produced in record time for SARS-CoV-2, offering the possibility of halting the global pandemic. However, inequalities in vaccine accessibility in different regions of the world create a need to increase international cooperation. Sputnik V is a recombinant adenovirus-based vaccine …

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Longitudinal Study after Sputnik V Vaccination Shows Durable SARS-CoV-2 Neutralizing Antibodies and Reduced Viral Variant Escape over Time

María Mora González López Ledesma, Lautaro Sánchez, Diego Sebastian Ojeda, Santiago Oviedo Rouco et autres

ABSTRACT Recent studies have shown a temporal increase in the neutralizing antibody potency and breadth to SARS-CoV-2 variants in coronavirus disease 2019 (COVID-19) convalescent individuals. Here, we examined longitudinal antibody responses and viral neutralizing capacity to the B.1 lineage virus (Wuhan related), …

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Sputnik V vaccine elicits seroconversion and neutralizing capacity to SARS-CoV-2 after a single dose

Andres Hugo Rossi, Diego Sebastian Ojeda, Augusto Varese, Lautaro Sánchez et autres

Massive vaccination offers great promise for halting the global COVID-19 pandemic. However, the limited supply and uneven vaccine distribution create an urgent need to optimize vaccination strategies. We evaluate SARS-CoV-2-specific antibody responses after Sputnik V vaccination of healthcare workers in Argentina, measuring …

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Extracellular acidosis enhances Zika virus infection both in human cells and ex-vivo tissue cultures from female reproductive tract

Augusto Varese, Ezequiel Dantas, Ana Luz Paletta, Wendy Fitzgerald et autres

Zika virus (ZIKV) is a flavivirus transmitted by mosquitoes of the genus Aedes, but unlike other flaviviruses, ZIKV can be sexually transmitted by vaginal intercourse. The healthy vaginal pH ranges from 4.0 to 6.0, reaching values of 6.0–7.0 after semen deposition. Here, …