Silke Hobbie

Pharmacological and pre-clinical safety profile of rSIV.F/HN, a hybrid lentiviral vector for cystic fibrosis gene therapy

Alena Moiseenko, Anthony Sinadinos, Ana Sergijenko, Kyriel M. Pineault et autres

Rationale and objective Cystic fibrosis (CF) is caused by mutations in the CF transmembrane conductance regulator (CFTR) gene. CFTR modulators offer significant improvements, but ∼10% of patients remain nonresponsive or are intolerant. This study provides an analysis of rSIV.F/HN, a lentiviral vector …

de, gb, us (code pays fourni par la source)

Challenges and Opportunities for Commercializing Technologies in the Pulmonary Arena: An Official American Thoracic Society Report

Milica Vukmirovic, Kambez Hajipouran Benam, Jason J. Rose, Scott M. TURNER et autres

"Translational medicine" has been a buzzword for over two decades. The concept was intended to be lofty, to reflect a new "bench-to-bedside" approach to basic and clinical research that would bridge fields, close gaps, accelerate innovation, and shorten the time and effort …

A selective CCR2 antagonist showed monocytes migration-independent analgesia in rat models of inflammatory pain

Laura Corradini, Silke Hobbie, Sara Frattini, Birgit Stierstorfer et autres

de, it (code pays fourni par la source)

Late asthmatic response is modulated by TRPA1 antagonists in ovalbumin-induced bronchoconstriction in anaesthetized guinea pigs

Thierry Bouyssou, Zoe Noakes, Silke Hobbie, Martin Fleck et autres

In animal models of asthma, ovalbumin (OVA) aerosol results in bronchoconstriction characterized by a histamine-related early asthmatic response (EAR) followed by a neuropeptide-related late asthmatic response (LAR) which can be modulated by transient receptor potential (TRP) channel A1 antagonists (Thorax. 2012, 67, …

de (code pays fourni par la source)

High-Content Analysis of CCR2 Antagonists on Human Primary Monocytes

Simone Kredel, Michael Werner Wolff, Silke Hobbie, Michael Bieler et autres

The monocyte chemoattractant protein 1 (MCP-1)-driven activation of CC-type chemokine receptor 2 (CCR2) is one of the early key events to induce monocyte migration toward centers of inflammation. In this work, the authors analyzed MCP-1 internalization into primary human monocytes using partially …

de (code pays fourni par la source)

Pharmacological Profiling of Chemokine Receptor–Directed Compounds Using High-Content Screening

Dorothea Haasen, Susanne Merk, Peter Seither, Domnic H. Martyres et autres

High-content screening, typically defined as automated fluorescence microscopy combined with image analysis, is now well established as a means to study test compound effects in cellular disease-modeling systems. In this work, the authors establish several high-content screening assays in the 384-well format …

de, au, ch (code pays fourni par la source)

Gene silencing with STAT6 specific siRNAs blocks eotaxin release in IL‐4/TNFα stimulated human epithelial cells

Jörg F. Rippmann, Andreas Schnapp, Andreas Weith, Silke Hobbie

Small interfering RNAs have evolved as effective tools for the study of gene functions. Here, we demonstrate the use of different siRNAs for the specific knock down of the STAT6 transcription regulator and the complete silencing of the downstream signaling pathway. The …

de (code pays fourni par la source)

Phosphorylation-dependent proline isomerization catalyzed by Pin1 is essential for tumor cell survival and entry into mitosis.

Jörg F. Rippmann, Silke Hobbie, Christine Daiber, Bernd Guilliard et autres

Pin1, a member of the parvulin family of peptidyl-prolyl cis-trans isomerases (PPIases) has been implicated in the G2-M transition of the mammalian cell cycle. Pin1 interacts with a series of mitotic phosphoproteins, including Polo-like kinase-1, Cdc25C, and Cdc27, and is thought to …

de (code pays fourni par la source)

The haemolysin‐secreting ShlB protein of the outer membrane of Serratia marcescens : determination of surface‐exposed residues and formation of ion‐permeable pores by ShlB mutants in artificial lipid bilayer membranes

Ulrich Könninger, Silke Hobbie, Roland Benz, Volkmar Braun

The ShlB protein in the outer membrane of Serratia marcescens is the only protein known to be involved in secretion of the ShlA protein across the outer membrane. At the same time, ShlB converts ShlA into a haemolytic and a cytolytic toxin. …

de (code pays fourni par la source)

YopJ of Yersinia pseudotuberculosis is required for the inhibition of macrophage TNF‐α production and downregulation of the MAP kinases p38 and JNK

Lance E. Palmer, Silke Hobbie, Jorge E. Galán, James B. Bliska

Exposure of macrophages to lipopolysaccharide (LPS) leads to production of the pro-inflammatory cytokine, tumour necrosis factor alpha (TNF-alpha). Previous studies have suggested that pathogenic Yersinia spp. inhibit LPS-mediated production of TNF-alpha in macrophages, and that one of the Yop proteins secreted by …

us (code pays fourni par la source)

Involvement of mitogen-activated protein kinase pathways in the nuclear responses and cytokine production induced by Salmonella typhimurium in cultured intestinal epithelial cells

Silke Hobbie, Li-Mei Chen, Roger J. Davis, Jorge E. Galán

Central to the pathogenesis of Salmonella typhimurium is its ability to engage the host cell in a two-way biochemical interaction. As a consequence of this interaction, a dedicated protein secretion system, termed type III, is activated in these bacteria and directs the …

us (code pays fourni par la source)

Specific phosphatidylethanolamine dependence of Serratia marcescens cytotoxin activity

Ralf Hertle, Sandra Brutsche, Wolfram Groeger, Silke Hobbie et autres

The cytolytic and haemolytic activity of Serratia marcescens is determined by the ShlA protein, which is secreted across the outer membrane with the aid of the ShlB protein. In the absence of ShlB, inactive ShlA* remains in the periplasm of Escherichia col …

de (code pays fourni par la source)