Brendan T. Deveney

A droplet microfluidics-based platform for generating target-specific, natively-paired immune libraries and identifying potent and developable antibodies

Anusuya Ramasubramanian, Brendan T. Deveney, Sarah A. Clark, Paula Boutin et autres

The human antibody repertoire is a promising source for therapeutic-grade antibodies. Yet current methods for strategically mining these B cell repertoires are stymied by throughput and chain pairing considerations. This study presents advancements in fluidics and molecular biology that enable the multi-step …

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Fluorophilic boronic acid copolymer surfactant for stabilization of complex emulsion droplets with fluorinated oil

Wu Zhang, Brendan T. Deveney, Jörg G. Werner, Stefano Aime et autres

A fluorosurfactant is synthesized by the copolymerization of fluoroacrylate and boronic acid acrylamide monomers to stabilize fluorinated oil droplets. The copolymer surfactant couples with diols or polyols in adjacent aqueous phase to form an ultrathin elastic interfacial film to rigidify the interface, …

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A biocompatible surfactant film for stable microfluidic droplets

Brendan T. Deveney, John A. Heyman, Raoul G Rosenthal, David A. Weitz et autres

Droplets serve as practical compartments for the analysis of individual biological species like nucleic acids and single cells due to the small size and ease of production of droplets. However, coalescence among droplets is a persistent challenge that often precludes the application …

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Nano-confinement induced nucleation of ice-VII at room temperature

Jonggeun Hwang, Dongha Shin, Brendan T. Deveney, Manhee Lee et autres

The hydrogen bond (HB) network of water under confinement has been predicted to have distinct structures from that of bulk water. However, direct measurement of the structure has not been achieved. Here, we present experimental evidence of confinement-induced ice formation in water. …

Tuning porosity of macroporous hydrogels enables rapid rates of stress relaxation and promotes cell expansion and migration

Bryan A. Nerger, Brendan T. Deveney, Junzhe Lou, Blake F. Hanan et autres

Extracellular matrix (ECM) viscoelasticity broadly regulates cell behavior. While hydrogels can approximate the viscoelasticity of native ECM, it remains challenging to recapitulate the rapid stress relaxation observed in many tissues without limiting the mechanical stability of the hydrogel. Here, we develop macroporous …

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Toward the quantification of α-synuclein aggregates with digital seed amplification assays

Tal Gilboa, Zoe Swank, Rohan Thakur, Russell A. Gould et autres

The quantification and characterization of aggregated α-synuclein in clinical samples offer immense potential toward diagnosing, treating, and better understanding neurodegenerative synucleinopathies. Here, we developed digital seed amplification assays to detect single α-synuclein aggregates by partitioning the reaction into microcompartments. Using pre-formed α-synuclein …

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Dynamic Microcapsules from Complex Emulsion Drops

J. Werner, Saraf Nawar, Brendan T. Deveney, David A. Weitz

Triggered gelation of microfluidic polymer droplets to identify and isolate viable antigen-speific immune cells

Brendan T. Deveney, Julie Brouchon, John A. Heyman, Yuan Yuan et autres

Dynamic Microcapsules with Rapid and Reversible Permeability Switching

Jörg G. Werner, Brendan T. Deveney, Saraf Nawar

Abstract Dynamic microcapsules are reported that exhibit shell membranes with fast and reversible changes in permeability in response to external stimuli. A hydrophobic anhydride monomer is employed in the thiol–ene polymerization as a disguised precursor for the acid‐containing shells; this enables the …

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Molecular imprinting using monomers with solid-state polymerization

Dmitry Pestov, Natalia Levit, V. Maniscalco, Brendan T. Deveney et autres

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