Femke Broere

A liposomal inverse vaccine protects mice from arthritis and experimental autoimmune encephalomyelitis

Naomi Benne, Daniel Sáenz Fernández, Deja Porenta, Laura Bolkaerts et autres

Chronic autoinflammatory diseases such as rheumatoid arthritis and multiple sclerosis lack curative therapies, and current immunosuppressive treatments often cause systemic off-target effects. To address this unmet need, we previously developed an “inverse vaccine” based on anionic phosphatidylglycerol liposomes carrying a disease-specific peptide …

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A 16-colour spectral flow cytometry panel to characterise T cell immunophenotypes in canine oral melanoma

Esther Hindriks, Estefanía Lozano−Andrés, Arno Roos, Maurice M.J.M. Zandvliet et autres

Abstract Advances in immunophenotyping of tumour-infiltrating lymphocytes (TILs) have improved our understanding of prognostic biomarkers and immune targets in human melanoma. However, whether the tumour-immune landscape in canine oral malignant melanoma (COMM) is concordant with that of human melanoma has not been …

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Collagen hydrolysates for dogs with osteoarthritis: Bringing a food myth from bowl to the bench and back

Niels Blees, Anatomie en fysiologie, Locomotion - Surgery (CA), CS_Locomotion et autres

Osteoarthritis (OA) is a common joint disease in dogs that involves long-term inflammation, breakdown of cartilage, and pain. Treatment mainly focuses on reducing symptoms through medication, changes in activity, and nutrition. Collagen hydrolysate (CH) is considered a potentially interesting nutritional supplement for …

Induction of SpCas9-Directed Immune Responses Using Lipid Nanoparticles and Identification of SpCas9-Derived T Cell Epitopes in C57BL/6 Mice

Deja Porenta, Naomi Benne, Alice Sijts, Femke Broere et autres

A major goal of clinically oriented CRISPR–Cas9-based applications is safe and effective in vivo gene editing (knockout or correction) with precise targeting. Substantial efforts have been devoted to the preclinical development of novel drug delivery platforms that enable efficient, targeted delivery. However, …

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A canine macrophage cell line that can be polarized provides a model for canine macrophage differentiation

Qingkang Lyu, Victor P. M. G. Rutten, Ildiko Van Rhijn, Willem van Eden et autres

Monocyte-like cell lines are valuable models for studying macrophage biology, yet a well-characterized canine macrophage model has been lacking. The canine histiocytic sarcoma-derived cell line 030D was assessed for its macrophage polarization potential by stimulation with IFN-γ + LPS (M1) or IL-4 …

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Host Defense Peptides in Porcine Immunity: Porcine Host Defense Peptides and SCFAs in Antimicrobial Defense

Fatemeh Pashaie, Infectious Diseases and Immunology - Immunology, Immunologie, Femke Broere et autres

Pig production provides nearly one-third of the world’s meat supply but faces major challenges from infectious diseases and the heavy use of antibiotics. Frequent antibiotic use contributes to antimicrobial resistance, making its reduction a priority for both animal and public health. One …

Detailed Splenic Single-Cell Biodistribution of Phosphatidylglycerol-Containing Liposomes

Deja Porenta, Estefanía Lozano-Andrés, Enrico Mastrobattista, Femke Broere et autres

Antigen-specific tolerance induction is a promising therapeutic strategy for autoimmune and chronic inflammatory diseases. This can be achieved by targeted activation of regulatory T and B cells via antigen-presenting cells (APCs) in a tolerogenic context. Anionic antigen-carrying liposomes have shown potential; however, …

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Evaluating VEGFR2 as a Target for Anti‐Tumour Therapy in Canine Melanoma

Esther Hindriks, Wilhelmina Bergmann, Aitor Martínez Ruiz, Maurice M.J.M. Zandvliet et autres

Vascular endothelial growth factor receptor 2 (VEGFR2) is a key target for anti-angiogenic oncotherapy, as inhibiting this receptor on tumour vasculature slows tumour development and enhances drug- and immune infiltration, improving therapy outcome. Although VEGFR2 is primarily expressed on endothelial cells (ECs), …

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Activation of chicken macrophages by isomalto/malto-polysaccharide (IMMP) is facilitated by toll-like receptor 4 (TLR4)

Adil Ijaz, Max Putman, Femke Broere, Victor P. M. G. Rutten et autres

Isomalto/malto-polysaccharide (IMMP) is a digestion resistant starch that has shown to increase SCFA production in the gut microbiota of human and mice to promote intestinal health. Like other prebiotics, it escapes enzymatic digestion in the small intestine and reaches the colon intact …

Detailed splenic single-cell biodistribution of phosphatidylglycerol-containing liposomes

Deja Porenta, Estefanía Lozano−Andrés, Enrico Mastrobattista, Femke Broere et autres

Abstract Antigen-specific tolerance induction is a promising therapeutic strategy for autoimmune and chronic inflammatory diseases. This can be achieved by targeted activation of regulatory T and B cells via antigen-presenting cells (APCs) in a tolerogenic context. Anionic antigen-carrying liposomes have shown potential, …

nl (code pays fourni par la source)

Activation of chicken macrophages by isomalto/malto-polysaccharide (IMMP) is facilitated by toll-like receptor 4 (TLR4)

Adil Ijaz, M.S. Putman, Femke Broere, Victor P. M. G. Rutten et autres

Isomalto/malto-polysaccharide (IMMP) is a digestion resistant starch that has shown to increase SCFA production in the gut microbiota of human and mice to promote intestinal health. Like other prebiotics, it escapes enzymatic digestion in the small intestine and reaches the colon intact …

nl, Afrique du Sud (code pays fourni par la source)

Direct immunomodulatory effects of DSPC:DSPG:CHOL liposomes on murine dendritic cells

Naomi Benne, Enrico Mastrobattista, Femke Broere

Autoimmune diseases affect around 7-9% of the population world-wide and there is no cure for these diseases. Delivering disease-causing self-antigens in a tolerance-enhancing manner can halt the inappropriate immune response that characterizes autoimmunity. This can be achieved by delivery systems such as …

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