Naomi Benne

A liposomal inverse vaccine protects mice from arthritis and experimental autoimmune encephalomyelitis

Naomi Benne, Daniel Sáenz Fernández, Deja Porenta, Laura Bolkaerts et autres

Chronic autoinflammatory diseases such as rheumatoid arthritis and multiple sclerosis lack curative therapies, and current immunosuppressive treatments often cause systemic off-target effects. To address this unmet need, we previously developed an “inverse vaccine” based on anionic phosphatidylglycerol liposomes carrying a disease-specific peptide …

nl, be (code pays fourni par la source)

Induction of SpCas9-Directed Immune Responses Using Lipid Nanoparticles and Identification of SpCas9-Derived T Cell Epitopes in C57BL/6 Mice

Deja Porenta, Naomi Benne, Alice Sijts, Femke Broere et autres

A major goal of clinically oriented CRISPR–Cas9-based applications is safe and effective in vivo gene editing (knockout or correction) with precise targeting. Substantial efforts have been devoted to the preclinical development of novel drug delivery platforms that enable efficient, targeted delivery. However, …

nl, de (code pays fourni par la source)

Detailed Splenic Single-Cell Biodistribution of Phosphatidylglycerol-Containing Liposomes

Deja Porenta, Estefanía Lozano-Andrés, Enrico Mastrobattista, Femke Broere et autres

Antigen-specific tolerance induction is a promising therapeutic strategy for autoimmune and chronic inflammatory diseases. This can be achieved by targeted activation of regulatory T and B cells via antigen-presenting cells (APCs) in a tolerogenic context. Anionic antigen-carrying liposomes have shown potential; however, …

nl, us (code pays fourni par la source)

Detailed splenic single-cell biodistribution of phosphatidylglycerol-containing liposomes

Deja Porenta, Estefanía Lozano−Andrés, Enrico Mastrobattista, Femke Broere et autres

Abstract Antigen-specific tolerance induction is a promising therapeutic strategy for autoimmune and chronic inflammatory diseases. This can be achieved by targeted activation of regulatory T and B cells via antigen-presenting cells (APCs) in a tolerogenic context. Anionic antigen-carrying liposomes have shown potential, …

nl (code pays fourni par la source)

Direct immunomodulatory effects of DSPC:DSPG:CHOL liposomes on murine dendritic cells

Naomi Benne, Enrico Mastrobattista, Femke Broere

Autoimmune diseases affect around 7-9% of the population world-wide and there is no cure for these diseases. Delivering disease-causing self-antigens in a tolerance-enhancing manner can halt the inappropriate immune response that characterizes autoimmunity. This can be achieved by delivery systems such as …

nl (code pays fourni par la source)

POS0743 HARNESSING TOLEROGENIC DENDRITIC CELLS FOR ANTIGEN-SPECIFIC IMMUNE MODULATION IN RHEUMATOID ARTHRITIS: FINDINGS FROM THE TOLERANT TRIAL

Arie Jan Stoppelenburg, Naomi Benne, Gerty Schreibelt, Laureen Lammers et autres

Background: Current treatments for rheumatoid arthritis primarily rely on immunosuppression, which carries significant long-term risks and fails to address the underlying immune dysregulation. Novel therapeutic strategies aim to restore self-tolerance by inducing antigen-specific regulatory T cells. We here report the initial findings …

nl (code pays fourni par la source)

PMAP-37: A versatile cathelicidin for neutralizing bacteria and viruses

Fatemeh Pashaie, Naomi Benne, Philippa I P Holzapfel, Tineke Veenendaal et autres

Antimicrobial peptides (AMPs), such as cathelicidins, show dual functionality by directly combating pathogens and indirectly eliminating them through stimulation of the immune system, generating interest in their therapeutic potential. Pigs have a large set of 11 cathelicidins, of which PMAP-37 is relatively …

nl (code pays fourni par la source)

Sequential STING and CD40 agonism drives massive expansion of tumor-specific T cells in liposomal peptide vaccines

Dmitrij Ostroumov, Naomi Benne, Fernando Lozano Vigario, Oscar Escalona-Rayo et autres

The clinical use of cancer vaccines is hampered by the low magnitude of induced T-cell responses and the need for repetitive antigen stimulation. Here, we demonstrate that liposomal formulations with incorporated STING agonists are optimally suited to deliver peptide antigens to dendritic …

de, nl (code pays fourni par la source)

Comparative analysis of lipid Nanoparticle-Mediated delivery of CRISPR-Cas9 RNP versus mRNA/sgRNA for gene editing in vitro and in vivo

Johanna Walther, Deja Porenta, Danny Wilbie, Cornelis Seinen et autres

The discovery that the bacterial defense mechanism, CRISPR-Cas9, can be reprogrammed as a gene editing tool has revolutionized the field of gene editing. CRISPR-Cas9 can introduce a double-strand break at a specific targeted site within the genome. Subsequent intracellular repair mechanisms repair …

nl, de (code pays fourni par la source)

Autoantigen‐Dexamethasone Conjugate‐Loaded Liposomes Halt Arthritis Development in Mice

Naomi Benne, Daniëlle ter Braake, Deja Porenta, Chun Yin Jerry Lau et autres

There is no curative treatment for chronic auto-inflammatory diseases including rheumatoid arthritis, and current treatments can induce off-target side effects due to systemic immune suppression. This work has previously shown that dexamethasone-pulsed tolerogenic dendritic cells loaded with the arthritis-specific antigen human proteoglycan …

nl, jp (code pays fourni par la source)

Supplementary Tables 1 and 2 and Supplementary Figures 1 through 7 from Efficient Eradication of Established Tumors in Mice with Cationic Liposome-Based Synthetic Long-Peptide Vaccines

Eleni Maria Varypataki, Naomi Benne, Joke A. Bouwstra, Wim Jiskoot et autres

Supplementary Table 1: Loading efficiencies of OVA24 and OVA17 in liposomes Supplementary fig.1: Loading of OVA17 and OVA24 in liposomes as function of adjustable initial peptide concentration Supplementary Table 2:Physicochemical properties of liposomes Supplementary fig.2:In vitro DC activation (A) and subsequent CD8+ …

Data from Efficient Eradication of Established Tumors in Mice with Cationic Liposome-Based Synthetic Long-Peptide Vaccines

Eleni Maria Varypataki, Naomi Benne, Joke A. Bouwstra, Wim Jiskoot et autres

Abstract Therapeutic vaccination with synthetic long peptides (SLP) can be clinically effective against HPV-induced premalignant lesions; however, their efficiency in established malignant lesions leaves room for improvement. Here, we report the high therapeutic potency of cationic liposomes loaded with well-defined tumor-specific SLPs …

nl (code pays fourni par la source)