Antonio R. Pombinho

Sub-Saharan African Prostate Cancer Patient-Derived Cell Lines and High-Throughput Drug Screening: Addressing Ancestry Underrepresentation in Oncobiology Research

Carla S. dos Santos, Ana C. Magalhães, Verônica Elizeu de Araújo Fernandes, Antonio R. Pombinho et autres

Background/Objectives: Prostate cancer (PC) exhibits marked disparities in incidence and mortality across ethnicities, with men of Sub-Saharan African (SSA) ancestry experiencing 1.7 and 2.0 times higher values, respectively, than European men (EUR). However, SSA preclinical models remain scarce (just one commercial cell …

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A high-throughput analysis of novel anti-fibrotics in human adult cardiac fibroblasts

Rita N. Gomes, Maria Cardona-Timoner, Elsa D. Silva, Antonio R. Pombinho et autres

Myocardial fibrosis, a hallmark of heart failure (HF), contributes to disease progression and mortality by impairing cardiac function. Despite the identification of potential anti-fibrotic molecules, there is a lack of effective pharmacological interventions for cardiac fibrosis due to poor translational relevance and …

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The spindle assembly checkpoint: Molecular mechanisms and kinase-targeted drug discovery

Inês Lima, Fernanda Borges, Antonio R. Pombinho, Daniel Chavarria

The spindle assembly checkpoint (SAC) is a surveillance mechanism required for the fidelity of chromosome segregation, ensuring that anaphase is not initiated until all chromosomes are properly attached to the mitotic spindle. In cancer cells, SAC inactivation leads to aneuploidy beyond the …

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Assay Development for High-Throughput Drug Screening Against Mycobacteria

Gabriel S. Oliveira, Clara M. Bento, Antonio R. Pombinho, Rita Santana dos Reis et autres

Mycobacterium abscessus (Mab) infections are challenging to treat due to high intrinsic drug resistance, comparable to multidrug-resistant tuberculosis. Treatments are extremely ineffective and based on a multi-drug regimen, resulting in low patient compliance. Consequently, the scientific community is urged to identify new …

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HMGA1 stimulates cancer stem-like features and sensitivity to monensin in gastric cancer

Diana Pádua, Paula Figueira, Antonio R. Pombinho, Inês Monteiro et autres

Gastric cancer represents a serious health problem worldwide, with insufficient molecular biomarkers and therapeutic options. Consequently, several efforts have been directed towards finding specific disease markers in order to develop new therapies capable of defeating gastric cancer. Attention has been pointed to …

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Monensin as potential drug for treatment of SLeX-positive tumors

Ana Filipa Costa, Emanuel Senra, Diana Campos, Isabel Faria‐Ramos et autres

Abstract Colorectal (CRC) and gastric (GC) cancers remain the top lethal cancers and targeted therapies in this setting are still very limited. Sialyl Lewis X (SLeX), a cancer-associated glycan highly expressed in both CRC and GC, plays a crucial role in cancer …

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High-Throughput Drug Screening Revealed That Ciclopirox Olamine Can Engender Gastric Cancer Stem-like Cells

Diana Pádua, Paula Figueira, Mariana Pinto, André Filipe Maia et autres

Cancer stem cells (CSCs) are relevant therapeutic targets for cancer treatment. Still, the molecular circuits behind CSC characteristics are not fully understood. The low number of CSCs can sometimes be an obstacle to carrying out assays that explore their properties. Thus, increasing …

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Data from Monensin Inhibits Canonical Wnt Signaling in Human Colorectal Cancer Cells and Suppresses Tumor Growth in Multiple Intestinal Neoplasia Mice

Lucie Tůmová, Antonio R. Pombinho, Martina Vojtěchová, Jitka Stančíková et autres

Abstract The Wnt signaling pathway is required during embryonic development and for the maintenance of homeostasis in adult tissues. However, aberrant activation of the pathway is implicated in a number of human disorders, including cancer of the gastrointestinal tract, breast, liver, melanoma, …

Supplementary Materials and Methods, Figures 1 - 9, Tables 1 - 5 from Monensin Inhibits Canonical Wnt Signaling in Human Colorectal Cancer Cells and Suppresses Tumor Growth in Multiple Intestinal Neoplasia Mice

Lucie Tůmová, Antonio R. Pombinho, Martina Vojtěchová, Jitka Stančíková et autres

PDF file - 515KB, Supplementary Materials and Methods. Supplementary Figure S1. Densitometric analysis of western blots shown in Figs. 2D (A), 3C (B) and 4D (C). Supplementary Figure S2. Monensin suppresses Wnt signaling activated by TALEN-induced disruption of the APC gene. Supplementary …

Supplementary Materials and Methods, Figures 1 - 9, Tables 1 - 5 from Monensin Inhibits Canonical Wnt Signaling in Human Colorectal Cancer Cells and Suppresses Tumor Growth in Multiple Intestinal Neoplasia Mice

Lucie Tůmová, Antonio R. Pombinho, Martina Vojtěchová, Jitka Stančíková et autres

PDF file - 515KB, Supplementary Materials and Methods. Supplementary Figure S1. Densitometric analysis of western blots shown in Figs. 2D (A), 3C (B) and 4D (C). Supplementary Figure S2. Monensin suppresses Wnt signaling activated by TALEN-induced disruption of the APC gene. Supplementary …

Data from Monensin Inhibits Canonical Wnt Signaling in Human Colorectal Cancer Cells and Suppresses Tumor Growth in Multiple Intestinal Neoplasia Mice

Lucie Tůmová, Antonio R. Pombinho, Martina Vojtěchová, Jitka Stančíková et autres

Abstract The Wnt signaling pathway is required during embryonic development and for the maintenance of homeostasis in adult tissues. However, aberrant activation of the pathway is implicated in a number of human disorders, including cancer of the gastrointestinal tract, breast, liver, melanoma, …

Mitotic DNA damage promotes chromokinesin-mediated missegregation of polar chromosomes in cancer cells

Marco Novais-Cruz, Antonio R. Pombinho, Mafalda Sousa, André Filipe Maia et autres

DNA damage response (DDR) during interphase involves active signaling and repair to ensure genomic stability. However, how mitotic cells respond to DNA damage remains poorly understood. Supported by correlative live-/fixed-cell microscopy, it was found that mitotic cells exposed to several cancer chemotherapy …

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