Kohki Okabe

Phase Separation Driven by Dynamic Interactions in the N-Terminal Intrinsically Disordered Region of the DEAD-Box RNA Helicase DDX3X

Yuki Toyama, Shinichiro Inakami, Masaharu Takarada, Kohki Okabe et autres

The formation of membraneless organelles via liquid-liquid phase separation (LLPS) of proteins and RNAs has emerged as a central mechanism of cellular compartmentalization to finely regulate biological processes. DDX3X, a member of the DEAD-box RNA helicase family, is one of the global …

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Membrane Dynamics-Mediated Heat Retention Dictates Economical Intracellular Energy Flow

Akira Murakami, T Sato, Haruya Suzuki, Takashi Funatsu et autres

Abstract Intracellular thermometry has revealed temperature gradients within living cells, indicating the presence of localized energy flow. Moreover, increasing evidence suggests that cells can autonomously regulate heat production and use this heat to support cellular functions. Although heat is generally assumed to …

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Seedling Quality and Temperature Responses of Sparsely Sown Rice in Nursery Boxes in the Tohoku Region

Maya Matsunami, Tomoya KUMACHI, Kohki Okabe, Atsushi Sakata et autres

稲作における営農面積の拡大や温暖化環境に対応した省力化技術に資するため，近年，機械化が実用可能になった疎播苗の特性とその温度応答について東北地域の主要銘柄品種を用いて調査した．試験1では岩手大学において「あきたこまち」を3月下旬から6月中旬にかけて5回に分けて播種を繰り返し，疎播（乾籾60 g 箱—1），標準（同120 g箱—1），密播（同240 g箱—1）の3条件で育苗した稚苗および中苗の苗質を調査した．疎播苗は積算地温の上昇に伴う葉齢進展や地上部および根乾物重の増加が標準や密播よりも大きかった．また，疎播苗は草丈あたりの乾物重が大きく，他の播種密度よりも高い苗充実度を示した．試験2の東北地域の主要銘柄品種における疎播苗 （同65～80 g箱—1） と慣行苗 （同120～160 g箱—1） の比較においても，疎播苗は栽培地や品種に関わらず，積算気温が400～700℃日の範囲において慣行苗よりも高い乾物重と苗充実度を示した．いずれの試験においても疎播苗は温度1℃あたりの乾物生産量が他の播種密度よりも大きく，このことが幅広い温度域における優れた乾物生産能や苗充実度に寄与していた．したがって，春の異常昇温や大規模経営体での移植作業の遅れに伴う育苗期間の延長など，育苗時の温度環境が上昇した場合でも疎播苗は優れた苗質を維持できることから，移植時期が長期となる大規模経営体や温暖化環境に適応した技術であると考えられる．

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Fluorescent Thermometers Based on Carbon Quantum Dots with Various Detection Modes for Intracellular Temperature Measurement

Yukiho Shimazaki, Shunsuke Chuma, Kota Shiraya, Yurina Nakane et autres

We report that carbon quantum dots (CQDs) synthesized via a hydrothermal process using anthraquinone derivatives and l-cysteine provide versatile detection modes, making them suitable for various experimental setups. By modification of the precursor structures, these CQDs can function as different types of …

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High-throughput FLIM flow cytometry with a continuous-wave laser

Hiroshi Kanno, Kotaro Hiramatsu, Hideharu Mikami, Atsushi Nakayashiki et autres

Fluorescence properties of 4-N,N-dimethylaminosulfonyl-2,1,3-benzoselenadiazole derivatives and their application in a fluorescent polymeric thermometer

Seiichi Uchiyama, Eiko Hamada, Kohki Okabe

• Fluorescence characteristics of six 2,1,3-benzoselenadiazoles are reported. • Their fluorescence characteristics are markedly dependent on the substituent group. • Solvent effects on fluorescence characteristics of three derivatives are observed. • A fluorescent polymeric thermometer is proposed as an application of the …

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BPS2025 - Elucidation of neuronal differentiation mechanisms by thermal signaling through control of intracellular local temperature

Shunsuke Chuma, Kohki Okabe, Yoshie Harada

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BPS2025 - High-speed intracellular temperature mapping reveals the existence of non-conductive dissipation of energy by heating

Masaharu Takarada, Takashi Funatsu, Kohki Okabe

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Publisher Correction: High-throughput fluorescence lifetime imaging flow cytometry

Hiroshi Kanno, Kotaro Hiramatsu, Hideharu Mikami, Atsushi Nakayashiki et autres

The original version of this Article contained an error in Fig. 1b, in which key dashed lines were missing in the phase delay plot, and an error in Fig. 1c, where the symbol π was incorrectly denoted. The correct version of Fig. …

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High-throughput fluorescence lifetime imaging flow cytometry

Hiroshi Kanno, Kotaro Hiramatsu, Hideharu Mikami, Atsushi Nakayashiki et autres

Flow cytometry is a vital tool in biomedical research and laboratory medicine. However, its accuracy is often compromised by undesired fluctuations in fluorescence intensity. While fluorescence lifetime imaging microscopy (FLIM) bypasses this challenge as fluorescence lifetime remains unaffected by such fluctuations, the …

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Non-diffusive slow heat dissipation induces high local temperature in living cells

Masaharu Takarada, Ryo Shirakashi, Masahiro Takinoue, Motohiko Ishida et autres

Abstract Recently, intracellular thermometry has revealed temperature variations within cells. Although the biological significance of intracellular temperature change is recognized, the physical principles of intracellular temperature change remain a mystery. Here, we investigate intracellular heat transfer through intracellular temperature mapping using a …

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Nanoscale dynamics and localization of single endogenous mRNAs in stress granules

Ko Sugawara, Shin‐nosuke Uno, Mako Kamiya, Akihiko Sakamoto et autres

Stress granules (SGs) are cytoplasmic messenger ribonucleoprotein granules transiently formed in stressed mammalian cells. Although SG components have been well characterized, detailed insights into the molecular behavior inside SGs remain unresolved. We investigated nanoscale dynamics and localization of endogenous mRNAs in SGs …

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