Hideharu Mikami

Image-scanning light-sheet microscopy for high-speed volumetric imaging of complex biological dynamics

Yusuke Tomina, Ayumu Ishijima, Yu Toyoshima, Hikaru Shishido et autres

Abstract Volumetric fluorescence microscopy is a powerful method for studying complex biological systems because it enables comprehensive observation of structural and physiological dynamics. In particular, light-sheet microscopy (LSM) is a leading option for real-time volumetric fluorescence imaging as it combines high imaging …

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Compact, widely tunable ultrashort burst pulse generator using four mirrors

Keitaro Shimada, Ayumu Ishijima, Etsuko Kobayashi, Hideharu Mikami et autres

Ultrashort burst laser pulses serve as powerful tools for precise laser processing, broadband ultrafast spectroscopy, and high-speed laser-scanning microscopy. However, the performance of conventional burst pulse generators is limited by constraints in the pulse time interval variability, pulse energy variability, pulse number …

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High-throughput FLIM flow cytometry with a continuous-wave laser

Hiroshi Kanno, Kotaro Hiramatsu, Hideharu Mikami, Atsushi Nakayashiki et autres

Compact, widely tunable ultrashort burst pulse generator using four mirrors

Keitaro Shimada, Ayumu Ishijima, Etsuko Kobayashi, Hideharu Mikami et autres

Ultrashort burst laser pulses serve as powerful tools for precise laser processing, broadband ultrafast spectroscopy, and high-speed laser-scanning microscopy. However, the performance of conventional burst pulse generators is limited by constraints in the pulse time interval variability, pulse energy variability, pulse number …

Publisher Correction: High-throughput fluorescence lifetime imaging flow cytometry

Hiroshi Kanno, Kotaro Hiramatsu, Hideharu Mikami, Atsushi Nakayashiki et autres

The original version of this Article contained an error in Fig. 1b, in which key dashed lines were missing in the phase delay plot, and an error in Fig. 1c, where the symbol π was incorrectly denoted. The correct version of Fig. …

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High-throughput fluorescence lifetime imaging flow cytometry

Hiroshi Kanno, Kotaro Hiramatsu, Hideharu Mikami, Atsushi Nakayashiki et autres

Flow cytometry is a vital tool in biomedical research and laboratory medicine. However, its accuracy is often compromised by undesired fluctuations in fluorescence intensity. While fluorescence lifetime imaging microscopy (FLIM) bypasses this challenge as fluorescence lifetime remains unaffected by such fluctuations, the …

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Large-volume focus control at 10 MHz refresh rate via fast line-scanning amplitude-encoded scattering-assisted holography

Atsushi Shibukawa, Ryota Higuchi, Gookho Song, Hideharu Mikami et autres

The capability of focus control has been central to optical technologies that require both high temporal and spatial resolutions. However, existing varifocal lens schemes are commonly limited to the response time on the microsecond timescale and share the fundamental trade-off between the …

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High-speed fluorescence microscopy for next-generation life science

Hideharu Mikami

Fluorescence microscopy is an essential technique in biomedical research because it can reveal morphological details of complex organisms. Over the past few decades, the spatial resolution of fluorescence microscopy has dramatically improved with super-resolution techniques, contributing significantly to the evolution of life …

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Large-volume focus control at 10 MHz refresh rate via fast line-scanning amplitude-encoded scattering-assisted holography

Atsushi Shibukawa, Higuchi Ryota, Gookho Song, Hideharu Mikami et autres

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Large-volume focus control at 10 MHz refresh rate via fast line-scanning amplitude-encoded scattering-assisted holography

Atsushi Shibukawa, Ryota Higuchi, Gookho Song, Hideharu Mikami et autres

The capability of focus control has been central to optical technologies that require both high temporal and spatial resolutions. However, existing varifocal lens schemes are commonly limited to the response time on the microsecond timescale and share the fundamental trade-off between the …

Virtual-freezing fluorescence imaging flow cytometry with 5-aminolevulinic acid stimulation and antibody labeling for detecting all forms of circulating tumor cells

Hiroki Matsumura, Larina Tzu‐Wei Shen, Akihiro Isozaki, Hideharu Mikami et autres

Circulating tumor cells (CTCs) are precursors to cancer metastasis. In blood circulation, they take various forms such as single CTCs, CTC clusters, and CTC-leukocyte clusters, all of which have unique characteristics in terms of physiological function and have been a subject of …

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High-Speed Single-Pixel Imaging for Biomedical Applications

Hideharu Mikami

We demonstrate high-speed single-pixel imaging is enabled by multiplexing techniques such as frequency-division multiplexing and time-division multiplexing. Specifically, we present frequency-time-division-multiplexed single-pixel imaging technique for high-speed fluorescence imaging of biological cells.

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