Sanne Hillenius

A culture platform to reconstitute human foetal testes

Celine M. Roelse, Carmen L De Mooij, Brigite Cabrita, Cai-Feng Fei et autres

Human germ cells (hGCs) are specified early during development and migrate to the developing gonads. After sex determination, gonadogenesis and gametogenesis continue in a sex-specific manner, giving rise to testes in males and ovaries in females. In males, the relationship between hGCs …

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Chromosome-specific aberrations, rather than general aneuploidy, may drive mouse embryonic stem cell-derived teratoma metastasis

Joaquin Montilla‐Rojo, Sanne Hillenius, Thomas F. Eleveld, Daniela Salvatori et autres

INTRODUCTION: Pluripotent stem cells (PSC) are known to recurrently acquire genetic aberrations including chromosomal aneuploidy during long-term culture of which the consequences remain largely unknown. A recent study reported that xenografting of aneuploid mouse embryonic stem cells (mESC) gives rise to teratomas …

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Depletion of TP53 in Human Pluripotent Stem Cells Triggers Malignant‐Like Behavior

Joaquin Montilla‐Rojo, Thomas F. Eleveld, Marnix van Soest, Sanne Hillenius et autres

Human pluripotent stem cells (hPSCs) tend to acquire genetic aberrations upon culture in vitro. Common aberrations are mutations in the tumor suppressor TP53, suspected to confer a growth-advantage to the mutant cells. However, their full impact in the development of malignant features …

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Feeder-free culture of human pluripotent stem cells drives MDM4-mediated gain of chromosome 1q

Dylan Stavish, Christopher J. Price, Gabriele Gelezauskaite, Haneen Alsehli et autres

Culture-acquired variants in human pluripotent stem cells (hPSCs) hinder their applications in research and clinic. However, the mechanisms that underpin selection of variants remain unclear. Here, through analysis of comprehensive karyotyping datasets from over 23,000 hPSC cultures of more than 1,500 lines, …

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Characterization of the human fetal gonad and reproductive tract by single-cell transcriptomics

Jasin Taelman, Sylwia Czukiewska, Ioannis Moustakas, Yolanda W. Chang et autres

During human fetal development, sex differentiation occurs not only in the gonads but also in the adjacent developing reproductive tract. However, while the cellular composition of male and female human fetal gonads is well described, that of the adjacent developing reproductive tract …

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Cytogenetic resource enables mechanistic resolution of changing trends in human pluripotent stem cell aberrations linked to feeder-free culture

Dylan Stavish, Christopher J. Price, Gabriele Gelezauskaite, K Léonhard et autres

Summary Since the first derivation of human pluripotent stem cells (hPSCs), the number of culture conditions has steadily increased, making hPSC culture more facile. Nonetheless, there remains the persistent issue of culture-acquired genetic changes, hampering the reproducibility of hPSC research and jeopardising …

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Cell–cell interactions during the formation of primordial follicles in humans

Sylwia Czukiewska, Xueying Fan, Aat A. Mulder, Talia Van Der Helm et autres

Gametogenesis is a complex and sex-specific multistep process during which the gonadal somatic niche plays an essential regulatory role. One of the most crucial steps during human female gametogenesis is the formation of primordial follicles, the functional unit of the ovary that …

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Efficient and scalable generation of primordial germ cells in 2D culture using basement membrane extract overlay

Arend W. Overeem, Yolanda W. Chang, Ioannis Moustakas, Celine M. Roelse et autres

Current methods to generate human primordial germ cell-like cells (hPGCLCs) from human pluripotent stem cells (hPSCs) can be inefficient, and it is challenging to generate sufficient hPGCLCs to optimize in vitro gametogenesis. We present a differentiation method that uses diluted basement membrane …

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Safety Issues Related to Pluripotent Stem Cell-Based Therapies: Tumour Risk

Sanne Hillenius, Joaquin Montilla‐Rojo, Thomas F. Eleveld, Daniela Salvatori et autres

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Efficient and scalable generation of primordial germ cells in 2D culture using basement membrane extract overlay

Arend W. Overeem, Yolanda W. Chang, Ioannis Moustakas, Celine M. Roelsen et autres

SUMMARY Current human primordial germ cell like cells (hPGCLCs) differentiation methods from human pluripotent stem cells (hPSCs) are inefficient, and it is challenging to generate sufficient hPGCLCs to optimize in vitro gametogenesis. We present a new differentiation method that uses diluted basement …

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Characterization of the human fetal rete region by single cell transcriptional analysis of gonads and mesonephros/epididymis

Jasin Taelman, Sylwia Czukiewska, Ioannis Moustakas, Yolanda W. Chang et autres

SUMMARY During development of the male reproductive tract, the human rete functions as a bridging structure between the seminiferous tubes and the efferent ducts of the epididymis. However, despite its significant contribution to human sex-specific gonadogenesis and future male fertility, the rete …

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Efficient and Scalable Generation of Primordial Germ Cells in 2D Culture Using Basement Membrane Extract Overlay

Arend W. Overeem, Yolanda W. Chang, Ioannis Moustakas, Celine M. Roelsen et autres

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