Jasin Taelman

Mucosal Macrophages Govern Intestinal Regeneration in Response to Injury

Ilias Moraitis, Jasin Taelman, Borja Arozamena, Loris Mularoni et autres

BACKGROUND & AIMS: Radiation-induced enteritis develops in cancer patients treated with radiotherapy in the abdominal and pelvic cavity, a condition that impairs their quality of life. Radiation injury depletes proliferative intestinal stem cells; in response to this, the epithelium activates a regenerative …

es (code pays fourni par la source)

Enhanced small intestinal organoid-derived epithelial cell adhesion and growth in organ-on-a-chip devices

Federica Quacquarelli, Jasin Taelman, Jordi Guiu, Maria Antfolk

, small intestinal organoid-derived epithelial cells. Hence, a need to functionalize and coat the surface has arisen to render it more hydrophilic and improve its ability to support cell adhesion and growth. While previous research has demonstrated some successful results in culturing …

se, es (code pays fourni par la source)

Characterization of the human fetal gonad and reproductive tract by single-cell transcriptomics

Jasin Taelman, Sylwia Czukiewska, Ioannis Moustakas, Yolanda W. Chang et autres

During human fetal development, sex differentiation occurs not only in the gonads but also in the adjacent developing reproductive tract. However, while the cellular composition of male and female human fetal gonads is well described, that of the adjacent developing reproductive tract …

nl, be (code pays fourni par la source)

Characterization of the human fetal rete region by single cell transcriptional analysis of gonads and mesonephros/epididymis

Jasin Taelman, Sylwia Czukiewska, Ioannis Moustakas, Yolanda W. Chang et autres

SUMMARY During development of the male reproductive tract, the human rete functions as a bridging structure between the seminiferous tubes and the efferent ducts of the epididymis. However, despite its significant contribution to human sex-specific gonadogenesis and future male fertility, the rete …

nl, be (code pays fourni par la source)

Human Intestinal Organoids: Promise and Challenge

Jasin Taelman, Mònica Dı́az, Jordi Guiu

The study of human intestinal biology in healthy and diseased conditions has always been challenging. Primary obstacles have included limited tissue accessibility, inadequate in vitro maintenance and ethical constrains. The development of three-dimensional organoid cultures has transformed this entirely. Intestinal organoids are …

es (code pays fourni par la source)

Improving In Vitro Culture of Human Male Fetal Germ Cells

Myriam Martin‐Inaraja, Mónica Ferreira, Jasin Taelman, Cristina Eguizábal et autres

Male human fetal germ cells (hFGCs) give rise to spermatogonial stem cells (SSCs), which are the adult precursors of the male gametes. Human SSCs are a promising (autologous) source of cells for male fertility preservation; however, in contrast to mouse SSCs, we …

es, nl, be (code pays fourni par la source)

P–803 Novel culture conditions for the improvement of the in vitro expansion of human male fetal germ cells

Carmen Martín, Melisane Regina Lima Ferreira, Jasin Taelman, Cristina Eguizábal et autres

Abstract Study question Do different ECMs/substrates and growth media culture conditions improve in vitro male human primordial germ cell (hPGC) expansion? Summary answer We achieved in vitro expansion improvement of male hPGCs with specific growth factors such as LIF, EGF, FGF2 and …

es, nl (code pays fourni par la source)

Sex-Specific Isolation and Propagation of Human Premeiotic Fetal Germ Cells and Germ Cell-Like Cells

Swati Mishra, Jasin Taelman, Yolanda W. Chang, Annekatrien Boel et autres

The second trimester of human development is marked by asynchronous gonadal development hampering the isolation of homogenous populations of early and late fetal germ cells (FGCs). We evaluated the feasibility of using surface markers TNAP, PDPN, EPCAM and ITGA6 to isolate FGCs …

be, nl (code pays fourni par la source)

Activin A-derived human embryonic stem cells show increased competence to differentiate into primordial germ cell-like cells

Swati Mishra, Jasin Taelman, Mina Popovic, Laurentijn Tilleman et autres

Protocols for specifying human primordial germ cell-like cells (hPGCLCs) from human embryonic stem cells (hESCs) remain hindered by differences between hESC lines, their derivation methods, and maintenance culture conditions. This poses significant challenges for establishing reproducible in vitro models of human gametogenesis. …

be, nl (code pays fourni par la source)

Navigating pluripotency : resetting the primed state and exploring histone modification dynamics in human embryonic stem cells

Jasin Taelman

Untargeted histone profiling during naive conversion uncovers conserved modification markers between mouse and human

Laura De Clerck, Jasin Taelman, Mina Popovic, Sander Willems et autres

Recent progress has enabled the conversion of primed human embryonic stem cells (hESCs) to the naive state of pluripotency, resembling the well-characterized naive mouse ESCs (mESCs). However, a thorough histone epigenetic characterization of this conversion process is currently lacking, while its likeness …

be, nl (code pays fourni par la source)

Extended In Vitro Culture of Human Embryos Demonstrates the Complex Nature of Diagnosing Chromosomal Mosaicism From a Single Trophectoderm Biopsy

Mina Popovic, L Dhaenens, Jasin Taelman, Annelies Dheedene et autres

(Abstracted from Hum Reprod 2019;34(4):758–769) The development of preimplantation genetic testing (PGT-A) in conjunction with assisted reproductive technology has led to valuable analysis of the high frequency of chromosomal abnormalities observed in human embryos. While mosaic embryos may lead to healthy live …

nl, be (code pays fourni par la source)