Zhong‐Ping Yao

Distinct Mechanisms for Inhibition of SARS-CoV-2 Main Protease: Dimerization Promoted by Peptidomimetic Inhibitors and Disrupted by Ebselen

Chengxi Liu, Qinyu Jia, Chang Zhao, Zhong‐Ping Yao

The SARS-CoV-2 main protease (Mpro) is a key target for antiviral drugs. Given its conserved sequence across coronaviruses and essential role in viral replication, numerous inhibitors have been developed to target its active site. Mpro exists in equilibrium between the active dimer …

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Distinct Mechanisms for Inhibition of SARS-CoV-2 Main Protease: Dimerization Promoted by Peptidomimetic Inhibitors and Disrupted by Ebselen

Chengxi Liu, Qinyu Jia, Chang Zhao, Zhong‐Ping Yao

The SARS-CoV-2 main protease (Mpro) is a key target for antiviral drugs. Given its conserved sequence across coronaviruses and essential role in viral replication, numerous inhibitors have been developed to target its active site. Mpro exists in equilibrium between the active dimer …

hk, cn (code pays fourni par la source)

Tenfold Expansion Submicrometer MALDI Mass Spectrometry Imaging of Tissues and Cultured Cells

Chengyi Xie, Jianing Wang, Lei Guo, Xin Diao et autres

Achieving submicrometer lateral resolution in matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is essential for molecular characterization at the single-cell and subcellular levels but is fundamentally limited by optical focusing and matrix crystallization. Here, we present tenfold expansion mass spectrometry imaging (10X …

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Tenfold Expansion Submicrometer MALDI Mass Spectrometry Imaging of Tissues and Cultured Cells

Chengyi Xie, Jianing Wang, Lei Guo, Xin Diao et autres

ABSTRACT Achieving submicrometer lateral resolution in matrix‐assisted laser desorption/ionization mass spectrometry imaging (MALDI‐MSI) is essential for molecular characterization at the single‐cell and subcellular levels but is fundamentally limited by optical focusing and matrix crystallization. Here, we present tenfold expansion mass spectrometry imaging …

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Resuscitation and survival time in terminal cancer patients at the end of life: A retrospective observational study in a Chinese hospital

Zhong‐Ping Yao, Hongxia Tan, Yue-He Tu, Shaowen Wang et autres

End-of-life care decisions for terminal cancer patients remain a clinical and ethical challenge, particularly regarding the use of aggressive interventions. This retrospective observational study aimed to evaluate the impact of different treatment schemes on survival time in terminal cancer patients. A total …

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SARS-CoV-2 Main Protease Inhibition Through Dimerization Promotion by Peptidomimetic Inhibitors and Disruption by Ebselen

Chengxi Liu, Qinyu Jia, Chang Zhao, Zhong‐Ping Yao

Abstract The SARS-CoV-2 main protease (M pro ) is a key antiviral target that equilibrates between an active dimer and inactive monomer, making the dimerization interface a promising therapeutic vulnerability. This study investigates M pro monomer-dimer equilibrium and conformational changes induced by …

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Development of an integrated searching system for rapid and accurate classification of edible oils by matrix-assisted laser desorption/ionization mass spectrometry

Michael Ho-Yin, Suying Li, Zhen-Yan Li, Ji-Na Wu et autres

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The Interdomain Loop Modulates Conformational Dynamics for the Antibiotic-resistant Activity of TEM-type Extended-spectrum β-lactamases

Tsz-Fung Wong, Pui‐Kin So, Wai-Po Kong, Zhong‐Ping Yao

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Uncovering cargo clients and accessory factors of AP-1 and AP-4 through vesicle proteomics

Ziqing Peng, Jingran Fan, Yang Liu, Qinyu Jia et autres

-Golgi network (TGN) is a crucial sorting station in the secretory pathway, where adaptor protein (AP) complexes ensure selective cargo packaging into transport vesicles. However, the complete repertoire of cargoes and regulators associated with individual AP complexes remains poorly defined. Intriguingly, AP-4-mediated …

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Association of pan-immune-inflammation value and atherogenic index of plasma with chronic coronary syndrome in non-alcoholic fatty liver disease patients

Bing Yu, Jianqi Zhao, Wenjing Zhang, Leigang Wang et autres

Background: Non-alcoholic fatty liver disease (NAFLD) is linked to a higher risk of cardiovascular disease, particularly chronic coronary syndrome (CCS). However, reliable biomarkers for early CCS risk stratification in NAFLD patients remain lacking. This study aims to assess the pan-immune-inflammation value (PIV) …

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Enhanced degradation of tetracycline by membrane-confined catalytic reaction within Co2CO3(OH)2/polyvinylidene fluoride

Jiankang Wang, Yajing Wang, Yuan Dong Peng, Zhong‐Ping Yao et autres

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Inhibitor-Dependent Tolerance of New Delhi Metallo-β-Lactamase Driven by Single Mutation-Induced Conformational Changes

Liwen Huang, Tsz-Fung Wong, Qipeng Cheng, Pui‐Kin So et autres

The emergence of metallo-β-lactamases as formidable adversaries in the antimicrobial resistance crisis stems from their unparalleled capacity to hydrolyze β-lactam antibiotics. This study deciphers the evolutionary strategy of New Delhi metallo-β-lactamase (NDM) variants through studies of conformational dynamics. We employ hydrogen/deuterium exchange …

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